苦瓜白粉病的生物防治及病程代谢物质含量变化
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摘要
利用枯草芽孢杆菌BLG010对苦瓜白粉病进行防治试验,同时为了探明苦瓜白粉病的病程相关物质,利用气相色谱-质谱联用技术分析白粉病菌接种处理、白粉病菌+BLG010接种处理和蒸馏水喷施处理后8 d和15 d时苦瓜叶片的代谢物质差异。试验结果表明,BLG010对苦瓜白粉病的防治效果高达89.49%;苦瓜感染白粉病菌后,D-葡萄糖、延胡索酸和肌醇半乳糖苷含量在8 d和15 d均上调表达,而甘氨酸、甘油酸、L-谷氨酸和咖啡酸则表现为下调。这7种代谢物质的含量变化与苦瓜白粉病病程相关,施用BLG010可以降低苦瓜白粉病病情指数,避免这7种代谢物质含量的异常变化。
This paper conducted experiment on controlling bitter gourd powdery mildew using Bacillus subtilis(BLG010).And in order to find out the pathogenic process-related substances,the paper analyzed the differences in metabolic substances of bitter gourd leaves 8 days and 15 days after pathogen inoculation treatment,pathogen+BLG010 inoculation treatment and distilled water spraying treatment by GC-MS methodology.The results showed that the control effect of BLG010 on bitter gourd powdery mildew was as high as 89.49%.After pathogen infection,the contents of D-glucose,fumaric acid and galactinol on 8 days and 15 days were all up-regulated.While,the contents of glycine,glyceric acid,L-glutamic acid and caffeic acid were down-regulated.Contents of these 7 metabolic substances were related to the pathogenic process of bitter gourd powdery mildew.Application of BLG010 can decrease the disease index,and avoid the abnormal changes in contents of these 7 metabolic substances.
This paper conducted experiment on controlling bitter gourd powdery mildew using Bacillus subtilis(BLG010).And in order to find out the pathogenic process-related substances,the paper analyzed the differences in metabolic substances of bitter gourd leaves 8 days and 15 days after pathogen inoculation treatment,pathogen+BLG010 inoculation treatment and distilled water spraying treatment by GC-MS methodology.The results showed that the control effect of BLG010 on bitter gourd powdery mildew was as high as 89.49%.After pathogen infection,the contents of D-glucose,fumaric acid and galactinol on 8 days and 15 days were all up-regulated.While,the contents of glycine,glyceric acid,L-glutamic acid and caffeic acid were down-regulated.Contents of these 7 metabolic substances were related to the pathogenic process of bitter gourd powdery mildew.Application of BLG010 can decrease the disease index,and avoid the abnormal changes in contents of these 7 metabolic substances.
引文
包伊凡,沈新春,汪芳.2018.咖啡酸及其主要衍生物的研究进展及开发前景.天然产物研究与开发,(10):1825-1833.
陈燕琼.2010.高温高湿条件下苦瓜白粉病的防治技术.长江蔬菜,(9):40-41.
邓小鹏.2010.超量积累植物龙葵(Solanum nigrum L.)对镉的吸收、积累及耐性机理研究[博士论文].南京:南京农业大学.
郭玉朋.2014.植物光呼吸途径研究进展.草业学报,23(4):322-329.
韩晓芳,彭柯力,吴海霞,宋珊珊,李亚辉,朱晔荣,白艳玲,王勇.2016.丝氨酸对光呼吸基因节律的影响机制初探.植物生理学报,52(9):1397-1405.
韩园园,庄艳,李春敏,赵永波,王忆,张新忠,韩振海.2012.苹果实生树枝干对轮纹病菌抗病性反应的差异.中国农业大学学报,17(5):69-75.
郜明泉,赵鹏飞,赵会杰,薛瑞丽,李华.2015.葡萄糖对干旱胁迫下小麦种子萌发及幼苗生长的调节作用.生命科学研究,19(1):59-61.
贺军花.2018.根皮苷和阿魏酸对苹果灰霉病抗性防御系统的影响[硕士论文].杨凌:西北农林科技大学.
胡玉林,左雪冬,步佳佳,王忠猛,谢江辉.2011.香蕉与枯萎病菌互作中3种酚类物质的含量变化研究.热带作物学报,32(12):2302-2306.
黄曦,许兰兰,黄荣韶,黄庶识.2010.枯草芽孢杆菌在抑制植物病原菌中的研究进展.生物技术通报,(1):24-29.
江胜德,郭兰,王邝佳,杨兰兰.2015.L-谷氨酸对白芨组培苗叶绿素含量·矿质元素吸收及生长的影响.安徽农业科学,43(20):143-145.
刘春,曹丽敏,周东升,彭晚霞.2013.干旱、盐分和温度胁迫诱导的植物代谢改变.生物技术通报,23(4):1-7.
刘斐,谭贻,王忆,吴婷,张新忠.2015.苹果腐烂病菌侵染过程中枝条相关病程物质含量的变化.中国农学通报,31(34):177-186.
刘美君.2016.线粒体交替氧化酶呼吸途径对植物叶片的光破坏防御作用及其调控机制[博士论文].泰安:山东农业大学.
刘晓蕾.2011.干旱下丹参酚酸类化合物积累机制及其抗旱性研究[硕士论文].杨凌:西北农林科技大学.
刘业霞,姜飞,张宁,王洪涛,艾希珍.2011.嫁接辣椒对青枯病的抗性及其与渗透调节物质的关系.园艺学报,38(5):903-910.
罗玉.2004.植物中的糖代谢及其相关酶.文山学院学报,17(2):155-159.
马莉,陈丽梅.2008.植物丝氨酸羟甲基转移酶基因研究进展.生物技术通报,(2):15-19.
苗慧莹,郭容芳,赵彦婷,汪俏梅.2012.芥子油苷代谢调控和葡萄糖信号转导研究进展.食品安全质量检测学报,3(5):367-372.
仇奕之,李宜珅,杨鹏军,张旭强,杨宁.2018.甘氨酸对不同胁迫条件下高山离子芥试管苗的保护作用.兰州大学学报:自然科学版,54(2):200-207.
孙弘,刘合刚,崔谨谨,刘小京,杨丽琳,徐进.2010.外源甘氨酸对镉胁迫下苜蓿幼苗生长和氧化损伤的影响.中国生态农业学报,18(5):1022-1025.
唐永萍,石亚莉,贺军花,马利菁,周会玲.2017.不同灰霉病抗性苹果果实中酚类物质代谢特征.西北植物学报,(3):510-516.
田丽波,商桑,杨衍,司龙亭.2015.苦瓜对白粉病的抗性与相关生理生化指标的关系.西北农业学报,24(9):166-173.
魏林,梁志怀,张屹,陈玉荣,胡雅辉.2016.苦瓜白粉病为害特点及综合防治.长江蔬菜,(7):51-52.
吴越.2014.柑橘黄龙病菌侵染对甜橙叶片糖代谢的影响研究[硕士论文].重庆:西南大学.
夏斐,范佩,张明菊,夏启中.2015.次生细胞壁在植物抗病中的作用研究进展.湖北农业科学,54(23):5797-5803.
徐保红,杨洁.2008.甘氨酸甜菜碱与植物抗胁迫能力.新疆大学学报:自然科学版,25(3):349-352.
杨佳丽.2017.L-谷氨酸对果实抗性的诱导作用及其相关机理研究[博士论文].杭州:浙江大学.
杨志晓,薛刚,丁燕芳,张小全,刘红峰,张杰,王轶,任学良,杨铁钊.2015.烟草赤星病胁迫对不同抗性品种光合特性和渗透调节的影响.中国烟草学报,(3):82-87.
张海娥,乐文全,徐金涛,刘金利,冉辛拓,李永红.2014.梨黑星病侵染过程中叶片相关病程物质含量的变化.河北农业科学,18(2):46-51.
朱艳蕾,米丽吾叶提·阿达力.2018.渗透胁迫条件下内生细菌对银砂槐种子萌发和主要碳水化合物含量的影响.植物资源与环境学报,27(3):18-24.
邹英宁,吴强盛,李艳,黄咏明.2014.丛枝菌根真菌对枳根系形态和蔗糖、葡萄糖含量的影响.应用生态学报,25(4):1125-1129.
Beneduzi A,Ambrosini A,Passaglia L M P.2012.Plant growthpromoting rhizobacteria(PGPR):their potential as antagonists and biocontrol agents.Genetics and Molecular Biology,35:1044-1051.
Chandrasekaran M,Chun S C.2016.Induction of defence-related enzymes in tomato(Solanum lycopersicum)plants treated with Bacillus subtilis CBR05 against Xanthomonas campestris pv.vesicatoria.Biocontrol Science and Technology,26(10):1-21.
Chen X H,Scholz R,Borriss M,Junge H,M?gel G,Kunz S,Borriss R.2009.Difficidin and bacilysin produced by plantassociated Bacillus amyloliquefaciens are efficient in controlling fire blight disease.Journal of Biotechnology,140(1):38-44.
Elanchezhiyan K,Keerthana U,Nagendran K S,Prabhukarthikeyan R,Prabakar K,Raguchander T,Karthikeyan G.2018.Multifaceted benefits of Bacillus amyloliquefaciens strain FBZ24in the management of wilt disease in tomato caused by Fusarium oxysporum f.sp.lycopersici.Physiological and Molecular Plant Pathology,103:92-101.
Eloh K,Sasanelli N,Maxia A,Caboni P.2016.Untargeted metabolomics of tomato plants after root-knot nematode infestation.Journal of Agricultural and Food Chemistry,64(29):5963-5968.
Santos C V,Falc?o I P,Pinto G C,Oliveira H,Loureiro J.2002.Nutrient responses and glutamate and proline metabolism in sunflower plants and calli under Na2SO4 stress.Journal of Plant Nutrition and Soil Science,165(3):366-372.
Shrestha B K,Karki H S,Groth D E,Jungkhun N,Ham J H.2016.Biological control activities of rice-associated Bacillus sp.strains against sheath blight and bacterial panicle blight of rice.PLoSOne,11(1):e0146764.
Xu S J,Park D H,Kim J Y,Kim B S.2016.Biological control of gray mold and growth promotion of tomato using Bacillus spp.isolated from soil.Tropical Plant Pathology,41(3):1-8.
Yang J,Sun C,Fu D,Yu T.2017.Test for L-glutamate inhibition of growth of Alternaria alternata by inducing resistance in tomato fruit.Food Chemistry,230:145-153.
Zushi K,Matsuzoe N.2015.Metabolic profile of organoleptic and health-promoting qualities in two tomato cultivars subjected to salt stress and their interactions using correlation network analysis.Scientia Horticulturae,184:8-17.
陈燕琼.2010.高温高湿条件下苦瓜白粉病的防治技术.长江蔬菜,(9):40-41.
邓小鹏.2010.超量积累植物龙葵(Solanum nigrum L.)对镉的吸收、积累及耐性机理研究[博士论文].南京:南京农业大学.
郭玉朋.2014.植物光呼吸途径研究进展.草业学报,23(4):322-329.
韩晓芳,彭柯力,吴海霞,宋珊珊,李亚辉,朱晔荣,白艳玲,王勇.2016.丝氨酸对光呼吸基因节律的影响机制初探.植物生理学报,52(9):1397-1405.
韩园园,庄艳,李春敏,赵永波,王忆,张新忠,韩振海.2012.苹果实生树枝干对轮纹病菌抗病性反应的差异.中国农业大学学报,17(5):69-75.
郜明泉,赵鹏飞,赵会杰,薛瑞丽,李华.2015.葡萄糖对干旱胁迫下小麦种子萌发及幼苗生长的调节作用.生命科学研究,19(1):59-61.
贺军花.2018.根皮苷和阿魏酸对苹果灰霉病抗性防御系统的影响[硕士论文].杨凌:西北农林科技大学.
胡玉林,左雪冬,步佳佳,王忠猛,谢江辉.2011.香蕉与枯萎病菌互作中3种酚类物质的含量变化研究.热带作物学报,32(12):2302-2306.
黄曦,许兰兰,黄荣韶,黄庶识.2010.枯草芽孢杆菌在抑制植物病原菌中的研究进展.生物技术通报,(1):24-29.
江胜德,郭兰,王邝佳,杨兰兰.2015.L-谷氨酸对白芨组培苗叶绿素含量·矿质元素吸收及生长的影响.安徽农业科学,43(20):143-145.
刘春,曹丽敏,周东升,彭晚霞.2013.干旱、盐分和温度胁迫诱导的植物代谢改变.生物技术通报,23(4):1-7.
刘斐,谭贻,王忆,吴婷,张新忠.2015.苹果腐烂病菌侵染过程中枝条相关病程物质含量的变化.中国农学通报,31(34):177-186.
刘美君.2016.线粒体交替氧化酶呼吸途径对植物叶片的光破坏防御作用及其调控机制[博士论文].泰安:山东农业大学.
刘晓蕾.2011.干旱下丹参酚酸类化合物积累机制及其抗旱性研究[硕士论文].杨凌:西北农林科技大学.
刘业霞,姜飞,张宁,王洪涛,艾希珍.2011.嫁接辣椒对青枯病的抗性及其与渗透调节物质的关系.园艺学报,38(5):903-910.
罗玉.2004.植物中的糖代谢及其相关酶.文山学院学报,17(2):155-159.
马莉,陈丽梅.2008.植物丝氨酸羟甲基转移酶基因研究进展.生物技术通报,(2):15-19.
苗慧莹,郭容芳,赵彦婷,汪俏梅.2012.芥子油苷代谢调控和葡萄糖信号转导研究进展.食品安全质量检测学报,3(5):367-372.
仇奕之,李宜珅,杨鹏军,张旭强,杨宁.2018.甘氨酸对不同胁迫条件下高山离子芥试管苗的保护作用.兰州大学学报:自然科学版,54(2):200-207.
孙弘,刘合刚,崔谨谨,刘小京,杨丽琳,徐进.2010.外源甘氨酸对镉胁迫下苜蓿幼苗生长和氧化损伤的影响.中国生态农业学报,18(5):1022-1025.
唐永萍,石亚莉,贺军花,马利菁,周会玲.2017.不同灰霉病抗性苹果果实中酚类物质代谢特征.西北植物学报,(3):510-516.
田丽波,商桑,杨衍,司龙亭.2015.苦瓜对白粉病的抗性与相关生理生化指标的关系.西北农业学报,24(9):166-173.
魏林,梁志怀,张屹,陈玉荣,胡雅辉.2016.苦瓜白粉病为害特点及综合防治.长江蔬菜,(7):51-52.
吴越.2014.柑橘黄龙病菌侵染对甜橙叶片糖代谢的影响研究[硕士论文].重庆:西南大学.
夏斐,范佩,张明菊,夏启中.2015.次生细胞壁在植物抗病中的作用研究进展.湖北农业科学,54(23):5797-5803.
徐保红,杨洁.2008.甘氨酸甜菜碱与植物抗胁迫能力.新疆大学学报:自然科学版,25(3):349-352.
杨佳丽.2017.L-谷氨酸对果实抗性的诱导作用及其相关机理研究[博士论文].杭州:浙江大学.
杨志晓,薛刚,丁燕芳,张小全,刘红峰,张杰,王轶,任学良,杨铁钊.2015.烟草赤星病胁迫对不同抗性品种光合特性和渗透调节的影响.中国烟草学报,(3):82-87.
张海娥,乐文全,徐金涛,刘金利,冉辛拓,李永红.2014.梨黑星病侵染过程中叶片相关病程物质含量的变化.河北农业科学,18(2):46-51.
朱艳蕾,米丽吾叶提·阿达力.2018.渗透胁迫条件下内生细菌对银砂槐种子萌发和主要碳水化合物含量的影响.植物资源与环境学报,27(3):18-24.
邹英宁,吴强盛,李艳,黄咏明.2014.丛枝菌根真菌对枳根系形态和蔗糖、葡萄糖含量的影响.应用生态学报,25(4):1125-1129.
Beneduzi A,Ambrosini A,Passaglia L M P.2012.Plant growthpromoting rhizobacteria(PGPR):their potential as antagonists and biocontrol agents.Genetics and Molecular Biology,35:1044-1051.
Chandrasekaran M,Chun S C.2016.Induction of defence-related enzymes in tomato(Solanum lycopersicum)plants treated with Bacillus subtilis CBR05 against Xanthomonas campestris pv.vesicatoria.Biocontrol Science and Technology,26(10):1-21.
Chen X H,Scholz R,Borriss M,Junge H,M?gel G,Kunz S,Borriss R.2009.Difficidin and bacilysin produced by plantassociated Bacillus amyloliquefaciens are efficient in controlling fire blight disease.Journal of Biotechnology,140(1):38-44.
Elanchezhiyan K,Keerthana U,Nagendran K S,Prabhukarthikeyan R,Prabakar K,Raguchander T,Karthikeyan G.2018.Multifaceted benefits of Bacillus amyloliquefaciens strain FBZ24in the management of wilt disease in tomato caused by Fusarium oxysporum f.sp.lycopersici.Physiological and Molecular Plant Pathology,103:92-101.
Eloh K,Sasanelli N,Maxia A,Caboni P.2016.Untargeted metabolomics of tomato plants after root-knot nematode infestation.Journal of Agricultural and Food Chemistry,64(29):5963-5968.
Santos C V,Falc?o I P,Pinto G C,Oliveira H,Loureiro J.2002.Nutrient responses and glutamate and proline metabolism in sunflower plants and calli under Na2SO4 stress.Journal of Plant Nutrition and Soil Science,165(3):366-372.
Shrestha B K,Karki H S,Groth D E,Jungkhun N,Ham J H.2016.Biological control activities of rice-associated Bacillus sp.strains against sheath blight and bacterial panicle blight of rice.PLoSOne,11(1):e0146764.
Xu S J,Park D H,Kim J Y,Kim B S.2016.Biological control of gray mold and growth promotion of tomato using Bacillus spp.isolated from soil.Tropical Plant Pathology,41(3):1-8.
Yang J,Sun C,Fu D,Yu T.2017.Test for L-glutamate inhibition of growth of Alternaria alternata by inducing resistance in tomato fruit.Food Chemistry,230:145-153.
Zushi K,Matsuzoe N.2015.Metabolic profile of organoleptic and health-promoting qualities in two tomato cultivars subjected to salt stress and their interactions using correlation network analysis.Scientia Horticulturae,184:8-17.