椰心叶甲对植物挥发化合物的触角电位与行为反应
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摘要
椰心叶甲[Brontispa longissima (Gestro)]聚集潜藏于寄主未展开的心叶中取食危害,药剂难于接触虫体成为该害虫防治的重要瓶颈。为了应用有刺激气味的植物挥发化合物,胁迫椰心叶甲逃出心叶而暴露,以触角电位技术和行为生测,筛选出对椰心叶甲有较强胁迫驱避作用的化合物。结果表明:9种植物挥发化合物中,椰心叶甲雌、雄成虫触角对肉桂醛电位反应最强,触角电位相对反应值分别达到了128.17±0.44和103.87±2.54;芳樟醇、α-蒎烯、薄荷脑电位反应较弱,触角电位相对反应值分别为33.28±1.18和15.95±0.91、31.90±0.69和16.86±0.33、32.90±1.60和14.55±0.51。除了松节油和1-庚醛,其余化合物刺激雌虫触角产生的电位相对反应值均显著大于雄虫。椰心叶甲雌、雄成虫触角电位相对反应值与肉桂醛的浓度呈正相关。行为生测发现,稀释5×104倍液以下的肉桂醛对椰心叶甲雌、雄成虫均有显著的驱避作用,且浓度越高,椰心叶甲定向反应率越高。在稀释1×102倍液时,驱避率分别达到了72.41%±3.16%和70.22%±3.22%,可见,肉桂醛对椰心叶甲成虫有较强刺激和胁迫作用。
Brontispa longissima(Gestro) congregates in the unfolded heart leaves of palm plants; therefore,it is difficult to control the pest because the pesticide cannot enter and contact the pest. In this paper,antennal potential technology and behavioral bioassays were used to screen compounds that exert strong stress on B. longissima,thereby forcing B. longissima to leave the heart leaves and expose them to the stimulating volatile plant compound odors. The results showed that the adult antennae had the strongest response to cinnamaldehyde among the 9 volatile compounds tested,with the relative reaction values of the electroantennogram reaching 128.17±0.44 and 103.87± 2.54. The lowest potential occurred for linalool,α-pinene,and menthol with relative reaction values of the electroantennogram reaching 33.28 ± 1.18 and 15.95 ± 0.91,31.90 ± 0.69 and 16.86 ± 0. 33,and 32. 90 ± 1. 60 and14.55±0.51,respectively. Except for turpentine and 1-heptenal,the relative reaction values of the electroantennogram produced by the rest of the compounds with the antennae of females were significantly greater than those of males. There was a positive correlation between the relative reaction values of the electroantennogram and the concentration of cinnamaldehyde for adult B. longissima. The behavioral bioassay showed that cinnamaldehyde that was diluted less than 5× 104 times significantly repelled adult B. longissima,and the higher concentration corresponded with a higher rate of the targeted response. When diluted 1×102 times,the repellent rate reached 72.41%±3.16% and 70.22%±3.22%. Thus,it can be seen that cinnamaldehyde had the strongest stimulatory and stress effects on adult B. longissima of the 9 compounds tested.
Brontispa longissima(Gestro) congregates in the unfolded heart leaves of palm plants; therefore,it is difficult to control the pest because the pesticide cannot enter and contact the pest. In this paper,antennal potential technology and behavioral bioassays were used to screen compounds that exert strong stress on B. longissima,thereby forcing B. longissima to leave the heart leaves and expose them to the stimulating volatile plant compound odors. The results showed that the adult antennae had the strongest response to cinnamaldehyde among the 9 volatile compounds tested,with the relative reaction values of the electroantennogram reaching 128.17±0.44 and 103.87± 2.54. The lowest potential occurred for linalool,α-pinene,and menthol with relative reaction values of the electroantennogram reaching 33.28 ± 1.18 and 15.95 ± 0.91,31.90 ± 0.69 and 16.86 ± 0. 33,and 32. 90 ± 1. 60 and14.55±0.51,respectively. Except for turpentine and 1-heptenal,the relative reaction values of the electroantennogram produced by the rest of the compounds with the antennae of females were significantly greater than those of males. There was a positive correlation between the relative reaction values of the electroantennogram and the concentration of cinnamaldehyde for adult B. longissima. The behavioral bioassay showed that cinnamaldehyde that was diluted less than 5× 104 times significantly repelled adult B. longissima,and the higher concentration corresponded with a higher rate of the targeted response. When diluted 1×102 times,the repellent rate reached 72.41%±3.16% and 70.22%±3.22%. Thus,it can be seen that cinnamaldehyde had the strongest stimulatory and stress effects on adult B. longissima of the 9 compounds tested.
引文
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[2]黄法余,梁广勤,梁琼超,等.椰心叶甲的检疫及防除[J].植物检疫,2000,14(3):158-160.
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[15]赵冬香,卢芙萍,莫圣书,等.几种植物挥发物对椰心叶甲成虫的触角电生理活性[J].热带作物学报,2006,27(3):66-69.
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[17]张振,迟德富,宇佳,等.青杨脊虎天牛对13种植物挥发物的电生理及行为反应[J].林业科学,2010,46(10):69-75.
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[19]SHEPHERD W P,HUBER D P W,SEYBOLD S J. Antennal responses of the western pine beetle,Dendroctonus brevicomis(Coleoptera:Curculionidae),to stem volatiles of its primary host,Pinus ponderosa,and nine sympatric nonhost angiospermsand conifers[J]. Chemoecology,2008,17(4):209-221.
[20]DELORME J D,PAYNE T L. Antennal olfactory responses of black turpentine beetle,Dendroctonus terebrans(Olivier),tobark beetle pheromones and host terpenes[J]. Journal of Chemical Ecology,1990,16(4):1 321-1 329.
[21]张嫣,王星霁,张玲玲,等.红缘天牛成虫对葡萄挥发物的EAG和行为反应[J].天津师范大学学报(自然科学版),2017,37(2):30-35.
[22]王梦馨,李辉仙,武文竹,等.假眼小绿叶蝉对茶梢挥发物的行为反应[J].应用昆虫学报,2016,53(3):507-515.
[23]马国峰.椰心叶甲对寄主植物嗅觉反应的研究[D].广州:华南农业大学,2011:1-2.
[24]樊慧,金幼菊,李继泉,等.引诱植食性昆虫的植物挥发性信息化合物的研究进展[J].北京林业大学学报,2004,26(3):76-81.
[2]黄法余,梁广勤,梁琼超,等.椰心叶甲的检疫及防除[J].植物检疫,2000,14(3):158-160.
[3]HASSAN E. Problems of applied entomology in Papua New Guinea[J]. Anzeigefur Schadlingskunde and Pflanzenschutz,1972,45(9):129-134.
[4]STAPLEY J H. Insect pests of coconuts in the Pacific region[J]. Outlook on Agriculture,1973,5(5):211-217.
[5]陈义群,黎仕波,黄宏辉,等.越南、瑙鲁、马尔代夫等国发生椰心叶甲[J].植物检疫,2004,18(1):30.
[6]余道坚,陈志粦.澳大利亚椰心叶甲的发生与检疫控制[J].世界农业,2002,276(4):36-37.
[7]陈义群,黄宏辉,林明光,等.椰心叶甲在国外的发生与防治[J].植物检疫,2004,18(4):250-253.
[8]张志祥,程东美,江定心,等.椰心叶甲的传播、危害及防治方法[J].昆虫知识,2004,41(6):522-526.
[9]周荣,曾玲,梁广文,等.椰心叶甲实验种群的生物学特性观察[J].昆虫知识,2004,41(4):336-339.
[10]吕宝乾,金启安,温海波,等.入侵害虫椰心叶甲的研究进展[J].应用昆虫学报,2012,49(6):1 708-1 715.
[11]ERLER F,ULUG I,YALCINKAYA B. Repellent activity of five essential oils against Culex pipiens[J]. Fitoterapi,2006,77(7/8):491-494.
[12]LEAL W S. Application of Gc-EAD to the determination of mosquito repellents derived from a plant,Cymbopogon citratus[J]. Journal of Asia Pacific Entomology,1998,1(1):217-221.
[13]TEULON D A J,PENMAN D R,RAMAKERS P M J. Volatiles chemicals for thrips(Thysanoptera:Thripidae)hostfindingand applications for thrips pest management[J]. Journal of Economic Entomology,1993,86(5):1 405-1 415.
[14]程红.青杨脊虎天牛触角感器类型及其对植物挥发物的反应[D].哈尔滨:东北林业大学,2006:12-16.
[15]赵冬香,卢芙萍,莫圣书,等.几种植物挥发物对椰心叶甲成虫的触角电生理活性[J].热带作物学报,2006,27(3):66-69.
[16]邢雪.榆紫叶甲触角感器及其功能化合物的筛选研究[D].长春:东北师范大学,2016:17-27.
[17]张振,迟德富,宇佳,等.青杨脊虎天牛对13种植物挥发物的电生理及行为反应[J].林业科学,2010,46(10):69-75.
[18]李鹏.四种害虫对14种植物挥发物的EAG和行为反应[D].哈尔滨:东北林业大学,2012:8-28.
[19]SHEPHERD W P,HUBER D P W,SEYBOLD S J. Antennal responses of the western pine beetle,Dendroctonus brevicomis(Coleoptera:Curculionidae),to stem volatiles of its primary host,Pinus ponderosa,and nine sympatric nonhost angiospermsand conifers[J]. Chemoecology,2008,17(4):209-221.
[20]DELORME J D,PAYNE T L. Antennal olfactory responses of black turpentine beetle,Dendroctonus terebrans(Olivier),tobark beetle pheromones and host terpenes[J]. Journal of Chemical Ecology,1990,16(4):1 321-1 329.
[21]张嫣,王星霁,张玲玲,等.红缘天牛成虫对葡萄挥发物的EAG和行为反应[J].天津师范大学学报(自然科学版),2017,37(2):30-35.
[22]王梦馨,李辉仙,武文竹,等.假眼小绿叶蝉对茶梢挥发物的行为反应[J].应用昆虫学报,2016,53(3):507-515.
[23]马国峰.椰心叶甲对寄主植物嗅觉反应的研究[D].广州:华南农业大学,2011:1-2.
[24]樊慧,金幼菊,李继泉,等.引诱植食性昆虫的植物挥发性信息化合物的研究进展[J].北京林业大学学报,2004,26(3):76-81.