种子老化对农牧老芒麦生理生化特性的影响
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摘要
为探究农牧老芒麦种子的老化规律,采用高温高湿(40℃,RH95%)人工老化法,研究不同老化时间(0 d、1 d、2 d、3d、4 d、5 d、6 d)对农牧老芒麦种子生理生化特性的影响。结果表明:农牧老芒麦种子发芽势、发芽率、发芽指数、活力指数、抗氧化酶活性和可溶性蛋白含量均随老化时间的延长而下降;而电导率、丙二醛含量、脯氨酸和可溶性糖逐渐升高。相关分析表明,种子活力指数与氧化物歧化酶、过氧化氢酶、过氧化物酶活性、可溶性蛋白含量呈极显著正相关;与丙二醛含量、脯氨酸含量、可溶性糖含量呈极显著负相关;与电导率呈显著负相关。因此抗氧化酶活性、丙二醛含量、脯氨酸含量、可溶性糖含量、可溶性蛋白含量可以反应农牧老芒麦的种子的老化程度,而电导率可用来反应农牧老芒麦种子老化的敏感指标。本研究结果将为农牧老芒麦种质资源的保护提供基础理论依据。
The objective of this study was to investigate the aging regulation of Elymus sibiricus seeds. The effects of different seed aging time( 0 d,1 d,2 d,3 d,4 d,5 d,6 d) on the physiological and biochemical characteristics of Elymus sibiricus by high temperature and humidity( 40 ℃,RH95%) artificial aging method. The results showed that the germination potential,germination rate,germination index,vigor index,antioxidant enzyme activity and the content of soluble protein were decreased gradually along with the aging time. Conductivity and the content ofmalondialdehyde,Proline,soluble sugar gradually increased. The correlation analysis showed that the seed vigor index was positively correlated with superoxide,peroxidase,catalase and the content of soluble protein,had negative correlation with malondialdehyde and the content of proline and soluble sugar,and negatively correlated with conductivity. Therefore,thantioxidant enzyme activity and the content of malondialdehyde,proline,soluble sugar and protein can characterize the aging of the seeds of Elymus sibiricus,While the conductivity can be used to reflect the sensitive indicators of aging of seeds of Elymus sibiricus. The results of this study will provide the basic theoretical basis for the protection of Elymus sibiricus germplasm resources.
The objective of this study was to investigate the aging regulation of Elymus sibiricus seeds. The effects of different seed aging time( 0 d,1 d,2 d,3 d,4 d,5 d,6 d) on the physiological and biochemical characteristics of Elymus sibiricus by high temperature and humidity( 40 ℃,RH95%) artificial aging method. The results showed that the germination potential,germination rate,germination index,vigor index,antioxidant enzyme activity and the content of soluble protein were decreased gradually along with the aging time. Conductivity and the content ofmalondialdehyde,Proline,soluble sugar gradually increased. The correlation analysis showed that the seed vigor index was positively correlated with superoxide,peroxidase,catalase and the content of soluble protein,had negative correlation with malondialdehyde and the content of proline and soluble sugar,and negatively correlated with conductivity. Therefore,thantioxidant enzyme activity and the content of malondialdehyde,proline,soluble sugar and protein can characterize the aging of the seeds of Elymus sibiricus,While the conductivity can be used to reflect the sensitive indicators of aging of seeds of Elymus sibiricus. The results of this study will provide the basic theoretical basis for the protection of Elymus sibiricus germplasm resources.
引文
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[11]孔令琪,王俊峰,刘国庆,等.苜蓿硬实种子对人工老化时间处理的生理响应[J].草地学报,2011,19(1):81-85.
[12]田茜,段乃彬,李群,等.玉米种子人工老化过程中超弱发光与生理代谢的关系[J].山东农业科学,2016,48(8):30-33.
[13]刘毓侠,王铁固.种子活力研究进展[J].玉米科学,2012,20(4):90-94.
[14]张众,王比德,吴渠来,等.农牧老芒麦的选育推广及栽培利用[J].中国草地,1995(4):29-32.
[15]Delouehe J C,Baskin C C.Accelerated aging techniques for pre-dicting the relative storability of seeds lot[J].Seed Sci Technol,1973,l(2):427-452.
[16]姚侠妹,张瑞娥,偶春,等.人工老化处理对桔梗种子生理生化特性的影响[J].西北农林科技大学学报:自然科学版,2015,43(02):203-209+216.
[17]韩建国.牧草种子学(2版)[M].北京:中国农业大学出版社,2011:123-127.
[18]杜锦,曹高燚,杨勇,等.人工老化对不同品种小麦种子生理生化特性的影响[J].天津农业科学,2016,22(1):32-36.
[19]王学奎.植物生理生化实验原理和技术(3版)[M].北京:高等教育出版社,2006:167-280.
[20]李合生.植物生理生化实验原理和技术(1版)[M].北京:高等教育出版社,2006:164-260.
[21]张治安.植物生理学实验指导(1版)[M].北京:中国农业科学技术出版社,2004:65-70.
[22]陈建勋,王晓峰.植物生理学实验指导(1版)[M].广州:华南理工大学出版社,2006:61-65.
[23]李品明,孙玉芳,杨丙贤,等.低温胁迫对黄连膜脂过氧化作用和抗氧化酶活性的影响[J].中国农学通报,2011,27(15):117-120.
[24]孔令琪.不同老化处理对燕麦种子生理、蛋白质及抗氧化基因的影响[D].北京:中国农业大学,2015.
[25]孔治有,刘叶菊,覃鹏.人工老化处理对小麦种子生理生化特性的影响[J].亚热带植物科学,2010,39(1):17-20.
[26]李淑梅,王付娟,董丽平,等.黑麦草老化处理后种子活力和生理生化特性的变化[J].黑龙江农业科学,2015,(1):133-135.
[27]颜启传.种子学(1版)[M].北京:中国农业出版社,2001:79-84.
[28]薄鹏飞,孙秀玲,孙同虎,等.Nacl胁迫对海滨木槿抗氧化系统和渗透调节的影响[J].西北植物学报,2008,28(1):113-118.
[29]禹晓梅,马文广,郑昀晔.烟草种子的老化规律研究[J].种子,2016,35(3):21-24.
[30]陈婧,李建平,李荣.人工老化处理对糯玉米种子生理生化特性的影响[J].西北农业学报,2016,25(6):857-862.
[31]Demirkaya M.Relationships between antioxidant enzymes and physiological variations occur during ageing of pepper seeds[J].Horticulture,Environment,and Biotechnology,2013,54(2):97-102.
[32]时丽冉,刘志华.干旱胁迫对苣荬菜抗氧化酶和渗透调节物质的影响[J].草地学报,2010,18(5):673-677.
[33]吴浩,周青平,颜红波,等.人工老化对两种披碱草属种子生理生化特性的影响[J].草业科学,2014,31(12):2251-2256.
[34]李春雷,马世骏,彭滨,等.人工老化对玉米种子活力指标、内含物质含量及生理指标的影响[J].吉林农业大学学报,2014,36(5):505-509+514.
[35]王明霞,易津,乌仁其木格.人工劣变处理对华北驼绒藜种子活力的影响[J].中国草地,2003,25(4):32-36.
[36]张苗苗,赵彦,云锦凤,等.老化处理对2种披碱草属牧草种子生理生化的影响[J].种子,2012,31(4):23-27.
[2]付艺峰,李鸿雁,黄帆,等.人工老化对老芒麦种子活力和生理生化变化的影响[J].植物遗传资源学报,2014,15(6):1360-1363.
[3]Raj D,Dahiya O S,Yadav A K,et al.Effect of natural ageing on biochemical changes in relation to seed viability in okra(Abelmoschus esculentus)[J].Indian Journal of Agricultural Sciences,2014,84(2):280-286.
[4]P.Kaewnaree.Effect of Accelerated Aging process on Seed Quality and Biochemical Changes in sweet Pepper(Capsicum annuum Linn.)Seeds[J].Biotechnology,2011,10(2):175-182.
[5]Pichardo-González J M,Ayala-Garay J,GonzálezHernández V A,et al.Fatty acids and physiological quality of tomatillo(Physalis philadelphica Lam.)seed during natural ageing[J].Chilean Journal of Agricultural Research,2014,74(4):391-396.
[6]Parkhey S,Naithani S C,Keshavkant S.ROS production and lipid catabolism in desiccating Shorea robusta seeds during aging[J].Plant Physiologyand Biochemistry,2012,57:261-267.
[7]王琼.人工老化家榆种子程序性死亡胞核形态学特征及信号转导途径初步研究[D].北京:北京林业大学,2012.
[8]刘霞,刘菲.蓝萼香茶菜种子人工老化过程中生理生化特性的研究[J].种子,2012,31(4):27-30.
[9]Song S Q,Fredlund K M,Moller I M.Changes in lowmolecular weight heat shock Protein 22 of mitochondria during high-temperature accelerated ageing of Beta vuigaris L.Seeds[J].Acta Phytophysiologica Sinica,2001,27(l):73-81.
[10]韩熠,周宇飞,高铭悦,等.人工老化处理对高粱种子萌发及生理生化特性的影响[J].种子,2015,34(5):9-12+16.
[11]孔令琪,王俊峰,刘国庆,等.苜蓿硬实种子对人工老化时间处理的生理响应[J].草地学报,2011,19(1):81-85.
[12]田茜,段乃彬,李群,等.玉米种子人工老化过程中超弱发光与生理代谢的关系[J].山东农业科学,2016,48(8):30-33.
[13]刘毓侠,王铁固.种子活力研究进展[J].玉米科学,2012,20(4):90-94.
[14]张众,王比德,吴渠来,等.农牧老芒麦的选育推广及栽培利用[J].中国草地,1995(4):29-32.
[15]Delouehe J C,Baskin C C.Accelerated aging techniques for pre-dicting the relative storability of seeds lot[J].Seed Sci Technol,1973,l(2):427-452.
[16]姚侠妹,张瑞娥,偶春,等.人工老化处理对桔梗种子生理生化特性的影响[J].西北农林科技大学学报:自然科学版,2015,43(02):203-209+216.
[17]韩建国.牧草种子学(2版)[M].北京:中国农业大学出版社,2011:123-127.
[18]杜锦,曹高燚,杨勇,等.人工老化对不同品种小麦种子生理生化特性的影响[J].天津农业科学,2016,22(1):32-36.
[19]王学奎.植物生理生化实验原理和技术(3版)[M].北京:高等教育出版社,2006:167-280.
[20]李合生.植物生理生化实验原理和技术(1版)[M].北京:高等教育出版社,2006:164-260.
[21]张治安.植物生理学实验指导(1版)[M].北京:中国农业科学技术出版社,2004:65-70.
[22]陈建勋,王晓峰.植物生理学实验指导(1版)[M].广州:华南理工大学出版社,2006:61-65.
[23]李品明,孙玉芳,杨丙贤,等.低温胁迫对黄连膜脂过氧化作用和抗氧化酶活性的影响[J].中国农学通报,2011,27(15):117-120.
[24]孔令琪.不同老化处理对燕麦种子生理、蛋白质及抗氧化基因的影响[D].北京:中国农业大学,2015.
[25]孔治有,刘叶菊,覃鹏.人工老化处理对小麦种子生理生化特性的影响[J].亚热带植物科学,2010,39(1):17-20.
[26]李淑梅,王付娟,董丽平,等.黑麦草老化处理后种子活力和生理生化特性的变化[J].黑龙江农业科学,2015,(1):133-135.
[27]颜启传.种子学(1版)[M].北京:中国农业出版社,2001:79-84.
[28]薄鹏飞,孙秀玲,孙同虎,等.Nacl胁迫对海滨木槿抗氧化系统和渗透调节的影响[J].西北植物学报,2008,28(1):113-118.
[29]禹晓梅,马文广,郑昀晔.烟草种子的老化规律研究[J].种子,2016,35(3):21-24.
[30]陈婧,李建平,李荣.人工老化处理对糯玉米种子生理生化特性的影响[J].西北农业学报,2016,25(6):857-862.
[31]Demirkaya M.Relationships between antioxidant enzymes and physiological variations occur during ageing of pepper seeds[J].Horticulture,Environment,and Biotechnology,2013,54(2):97-102.
[32]时丽冉,刘志华.干旱胁迫对苣荬菜抗氧化酶和渗透调节物质的影响[J].草地学报,2010,18(5):673-677.
[33]吴浩,周青平,颜红波,等.人工老化对两种披碱草属种子生理生化特性的影响[J].草业科学,2014,31(12):2251-2256.
[34]李春雷,马世骏,彭滨,等.人工老化对玉米种子活力指标、内含物质含量及生理指标的影响[J].吉林农业大学学报,2014,36(5):505-509+514.
[35]王明霞,易津,乌仁其木格.人工劣变处理对华北驼绒藜种子活力的影响[J].中国草地,2003,25(4):32-36.
[36]张苗苗,赵彦,云锦凤,等.老化处理对2种披碱草属牧草种子生理生化的影响[J].种子,2012,31(4):23-27.