淘米水投加对花生渣厌氧发酵产酸过程的影响
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摘要
在发酵温度为35℃和原料干物质(TS计)质量固定的条件下,研究了淘米水的投加对花生渣厌氧发酵过程中有机质溶出的影响。试验设置淘米水与花生渣干物质比(TS)分别为1∶0(1#),1∶1(2#),1∶1.5(3#),1∶2(4#),1∶3(5#)和0∶1(6#)共6个处理。试验结果表明:淘米水的投加不能加速花生渣内蛋白质的溶解,且随着TS的升高,蛋白质溶出率及降解率降低;淘米水的投加明显提高了花生渣内碳水化合物的降解速率、挥发酸(VFAs)的产量及酸化率。当TS为1∶2时,4#试验组碳水化合物的降解速率、VFAs浓度及产率最高,分别为0.010/d,14 497.460 mg/L和0.54 g/g(VS),酸化率高达83.4%,产酸量为1#试验组与6#试验组总产量的1.36倍,提高了36.0%。淘米水与花生渣联合发酵以产偶数酸发酵为主导,占其总酸含量的90.0%,且不能通过调节TS配比的方式改变其发酵产酸类型。
On the basis of temperature of 35 ℃ and fixed total solid(TS) of substrates, the effects of dosing rice wash on hydrolysis and acidification of peanut residue were investigated. The experiment set up 6 treatments for rice water and peanut residue, the total solid(TS) ratios were set as 1∶0(1#), 1∶1(2#), 1∶1.5(3#), 1∶2(4#), 1∶3(5#) and 0∶1(6#), respectively. The results indicated that the rice wash can not accelerate the dissolution of protein in peanut residue, with the increase of TS ratio, protein dissolution and degradation rate were lower, but rice wash helped to improve the degradation of carbohydrates rate and acidification in peanut residue. The acidification effect was best when the mixing ratio of rice wash to peanut residue was 1 ∶2, with the degradation of carbohydrates rate reached 0.010/d, volatile fatty acids(VFAs) content of 14 497.460 mg/L and the yield was 0.54 g/g.VS, and acidification rate is as high as 83.4%, its produce acid quantity for a single material 1# and 6# 1.36 times of production, increased by 36.0%; The combined fermentation of rice wash and peanut residue is dominated by even acid fermentation, which accounts for up to90.0% of total acid content, and cannot affect the fermentation production acid type by regulating the ratio of TS.
On the basis of temperature of 35 ℃ and fixed total solid(TS) of substrates, the effects of dosing rice wash on hydrolysis and acidification of peanut residue were investigated. The experiment set up 6 treatments for rice water and peanut residue, the total solid(TS) ratios were set as 1∶0(1#), 1∶1(2#), 1∶1.5(3#), 1∶2(4#), 1∶3(5#) and 0∶1(6#), respectively. The results indicated that the rice wash can not accelerate the dissolution of protein in peanut residue, with the increase of TS ratio, protein dissolution and degradation rate were lower, but rice wash helped to improve the degradation of carbohydrates rate and acidification in peanut residue. The acidification effect was best when the mixing ratio of rice wash to peanut residue was 1 ∶2, with the degradation of carbohydrates rate reached 0.010/d, volatile fatty acids(VFAs) content of 14 497.460 mg/L and the yield was 0.54 g/g.VS, and acidification rate is as high as 83.4%, its produce acid quantity for a single material 1# and 6# 1.36 times of production, increased by 36.0%; The combined fermentation of rice wash and peanut residue is dominated by even acid fermentation, which accounts for up to90.0% of total acid content, and cannot affect the fermentation production acid type by regulating the ratio of TS.
引文
[1]张凤清,张海悦,陆鸣,等.花生渣的综合利用[J].食品科技,2004(1):89-90,97.
[2]周颖,张克强,刘丽丽,等.木质纤维素类农林加工废弃物厌氧发酵产甲烷潜力研究[J].可再生能源,2015,33(4):586-591.
[3]郭志伟,李勇,倪海亮.投配率对餐厨垃圾与污泥二级高温厌氧发酵产甲烷的影响[J].可再生能源,2015,33(2):314-319.
[4]熊丹丹.废弃碳源定向产酸过程调控与合成PHA的应用研究[D].哈尔滨:哈尔滨工业大学,2016.
[5]刘和,刘晓玲,邱坚,等.C/N对污泥厌氧发酵产酸类型及代谢途径的影响[J].环境科学学报,2010,30(2):340-346.
[6]郭晓慧.餐厨垃圾厌氧消化产甲烷工艺特性及其微生物学机理研究[D].杭州:浙江大学,2014.
[7]戈雷,葛大兵.城市家庭生活污水水量调查与水质分析[J].环境科学与管理,2010,35(2):16-17.
[8]国家环境保护总局.水和废水监测分析方法(第四版)(1)[M].北京:中国环境科学出版社,2002.
[9] Marion M Branford.A rapid and sensitive method for the quantitation of microgram quantities of protein dye binding[J].Analytical Biochemistry,1976,72(1/2):248-254.
[10] Dubois M,G K H J.Colorimetric method for determination of sugars and related substances[J].Analytical Biochemistry,1956,28(3):350-356.
[11]张玉静.餐厨垃圾厌氧水解产挥发性脂肪酸技术研究[D].北京:清华大学,2013.
[12] H Yuan,Y Chen,H Zhang,et al.Improved bio-production of short-chain fatty acids(SCFAs)from excess sludge Under alkaline conditions[J].Environmental Science&Technology,2006,40(6):2025-2029.
[13]赵丹,任南琪,王爱杰,等.PH、ORP制约的产酸相发酵类型及顶级群落[J].重庆环境科学,2003,25(2):33-35,38
[14]任南琪,赵丹,李建政,等.厌氧生物处理丙酸产生和积累的原因及控制对策[J].中国科学(B辑化学),2002,32(1):83-89.
[2]周颖,张克强,刘丽丽,等.木质纤维素类农林加工废弃物厌氧发酵产甲烷潜力研究[J].可再生能源,2015,33(4):586-591.
[3]郭志伟,李勇,倪海亮.投配率对餐厨垃圾与污泥二级高温厌氧发酵产甲烷的影响[J].可再生能源,2015,33(2):314-319.
[4]熊丹丹.废弃碳源定向产酸过程调控与合成PHA的应用研究[D].哈尔滨:哈尔滨工业大学,2016.
[5]刘和,刘晓玲,邱坚,等.C/N对污泥厌氧发酵产酸类型及代谢途径的影响[J].环境科学学报,2010,30(2):340-346.
[6]郭晓慧.餐厨垃圾厌氧消化产甲烷工艺特性及其微生物学机理研究[D].杭州:浙江大学,2014.
[7]戈雷,葛大兵.城市家庭生活污水水量调查与水质分析[J].环境科学与管理,2010,35(2):16-17.
[8]国家环境保护总局.水和废水监测分析方法(第四版)(1)[M].北京:中国环境科学出版社,2002.
[9] Marion M Branford.A rapid and sensitive method for the quantitation of microgram quantities of protein dye binding[J].Analytical Biochemistry,1976,72(1/2):248-254.
[10] Dubois M,G K H J.Colorimetric method for determination of sugars and related substances[J].Analytical Biochemistry,1956,28(3):350-356.
[11]张玉静.餐厨垃圾厌氧水解产挥发性脂肪酸技术研究[D].北京:清华大学,2013.
[12] H Yuan,Y Chen,H Zhang,et al.Improved bio-production of short-chain fatty acids(SCFAs)from excess sludge Under alkaline conditions[J].Environmental Science&Technology,2006,40(6):2025-2029.
[13]赵丹,任南琪,王爱杰,等.PH、ORP制约的产酸相发酵类型及顶级群落[J].重庆环境科学,2003,25(2):33-35,38
[14]任南琪,赵丹,李建政,等.厌氧生物处理丙酸产生和积累的原因及控制对策[J].中国科学(B辑化学),2002,32(1):83-89.