壳聚糖酶的研究进展
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摘要
壳聚糖酶(EC3.2.1.132)是一种糖基水解酶,主要来源于细菌和真菌,以内切方式催化水解部分乙酰化壳聚糖中的β-1,4-氨基葡萄糖苷键,生成壳寡糖。由于壳聚糖酶可以用于制备具有独特生物活性的物质——壳寡糖,因而在医药、食品、化妆品等领域有广泛的应用,也引起越来越多的科研人员对该酶的关注。文章总结了壳聚糖酶在生化特性、遗传改良及其应用等方面的最新研究进展。
Chitosanase(EC 3.2.1.132)is a glycosyl hydrolase mainly derived from bacteria and fungi.It catalyzes the hydrolysis ofβ-1,4-aminoglycoside bonds in partially acetylated chitosan to form chitosan oligosaccharides.Chitosan oligosaccharides have been widely used in medicine,food,cosmetics and other fields because they can be used to prepare chitosan oligosaccharides with unique biological activities.This paper summarizes the latest research progress of chitosanase in biochemical characteristics,genetic improvement and its application.
Chitosanase(EC 3.2.1.132)is a glycosyl hydrolase mainly derived from bacteria and fungi.It catalyzes the hydrolysis ofβ-1,4-aminoglycoside bonds in partially acetylated chitosan to form chitosan oligosaccharides.Chitosan oligosaccharides have been widely used in medicine,food,cosmetics and other fields because they can be used to prepare chitosan oligosaccharides with unique biological activities.This paper summarizes the latest research progress of chitosanase in biochemical characteristics,genetic improvement and its application.
引文
[1]Kim S K,Rajapakse N.Enzymatic production and biological activities of chitosan oligosaccharides(COS):a review[J].arbohydrate Polymers,2005,62(4):357-368.
[2]SomashekarD, JosephR. Chitosanases-propertiesand applications:a review[J].Bioresource Technology,1996,55(1):35-45.
[3]Gao X A,Ju W T,Jung W J,et al.Purification and characterization of chitosanase from Bacillus cereus D-11[J].Carbohydrate Polymers,2007,72(3):513-520.
[4]Wang S L,Chao C H,Liang T W,et al.Purification and characterization of protease and chitinase from Bacillus cereus TKU006and conversion of marine wastes by these enzymes[J].Marine Biotechnology,2009,11(3):334-344.
[5]Zitouni M,Fortin M,Scheerle R K,et al.Biochemical and molecular characterization of a thermostable chitosanase produced by the strain Paenibacillus sp.1794newly isolated from compost[J].Applied Microbiology and Biotechnology,2013,97(13):5801-5813.
[6]Wang S L,Peng J H,Liang T W,et al.Purification and characterization of a chitosanase from Serratia marcescens TKU011[J].Carbohydrate Research,2008b,343(8):1316-1323.
[7]Liu Y L,Jiang S,Ke Z M,et al.Recombinant expression of a chitosanase and its application in chitosan oligosaccharide production[J].Carbohydrate Research,2009,344(6):815-819.
[8]Johnsen M G,Hansen O C,Stougaard P.Isolation,characterization and heterologous expression of a novel chitosanase from Janthino bacterium sp.strain 4239[J].Microbial Cell Factories,2010,9(1):1-5.
[9]Wang S L,Tseng W N,Liang T W.Biodegradation of shellfish wastes and production of chitosanases by a squid pen-assimilating bacterium,Acinetobacter calcoaceticus TKU024[J].Biodegradation,2011,22(5):939-948.
[10]Jiang X,Chen D,Chen L,et al.Purification,characterization and action mode of a chitosanase from Streptomyces roseolus induced by chitin[J].Carbohydrate Research,2012,355(7):40-44.
[11]Zhang H,Sang Q,Zhang W.Statistical optimization of chitosanase production by Aspergillus sp. QD-2 in submerged fermentation[J].Annals of Microbiology,2012,62(1):193-201.
[12]Wang J,Zhou W,Yuan H,et al.Characterization of a novel fungal chitosanase Csn2from Gongronella sp.JG[J].Carbohydrate Research,2008,343(15):2583-2588.
[13]Zhou W,Yuan H,Wang J,et al.Production,purification andcharacterizationofchitosanaseproducedby Gongronellasp.JG[J].Letters in Applied Microbiology,2008,46(1):49-54.
[14]Da Silva L C,Honorato T L,Franco T T,et al.Optimization of chitosanase production by Trichoderma koningii sp.under solid-state fermentation[J].Food and Bioprocess Technology,2012(5):1564-1572.
[15]Gupta V,Prasanna R,Srivastava A K,et al.Purification and characterization of a novel antifungal endo-type chitosanase from Anabaena fertilissima[J].Annals of Microbiology,2012,62(3):1089-1098.
[16]Hsu S K,Chung Y C,Chang C T,et al.Purification and characterization of two chitosanase isoforms from the sheaths of bamboo shoots[J].Journal of Agricultural and Food Chemistry,2012,60(2):649-657.
[17]Fukamizo T,Fleury A C N,Mitsutomi M,et al.Exo-β-Dglucosaminidase from Amycolatopsis orientalis:catalytic residues,sugar recognition specificity, kinetics, and synergism[J].Glycobiology,2006,16(11):1064-1072.
[18]Chen X M,Zhai C,Kang L,et al.High-level expression and characterization of a highly thermostable chitosanase from Aspergillus fumigatus in Pichia pastoris[J].Biotechnology Letters,2012,34(4):689-694.
[19]Marcotte E M,Monzingo A F,Ernst S R.X-ray structure of an anti-fungal chitosanase fromStreptomyces N174[J].Nature Structural Biology,1996,3(2):155-162.
[20]Saito J,Kita A,Higuchi Y,et al.Crystal structure of chitosanase from Bacillus circulans MH-K1at 1.6-AA resolution and its substrate recognition mechanism[J].Journal of Biological Chemistry,1999,274(43):30818-30825.
[21]Liu G L,Li Y,Zhou H X,et al.Over-expression of a bacterial chitosanase gene in Yarrowia lipolytica and chitosan hydrolysis by the recombinant chitosanase[J].Journal of Molecular Catalysis B:Enzymatic,2012,83(11):100-107.
[22]Liu H,Bao X.Characterization of a chitosanase from Fusarium solani and its expression in an industrial strain of Saccharomyces cerevisiae[J]. Acta Microbiologica Sinica,2009,49(12):1607-1612.
[23]Kilani-Feki O,Frikha F,Zouari I,et al. Heterologous expression and secretion of an antifungal Bacillus subtilis chitosanase(CSNV26)in Escherichia coli[J].Bioprocess and Biosystems Engineering,2013,36(7):1-8.
[24]Zhu X F,Tan H Q,Zhu C,et al.Cloning and overexpression of a new chitosanase gene fromPenicilliumsp.D-1[J].AMB Express,2012,2(1):1-8.
[25]Ming M,Kuroiwa T,Ichikawa S,et al.Production of chitosanoligosaccharidesbychitosanasedirectly immobilized on an agar gelcoated multidisk impeller[J].Biochemical Engineering Journal,2006,28(3):289-294.
[26]Suresh P V.Biodegradation of shrimp processing bio-waste and concomitant production of chitinase enzyme and N-acetyl-D-glucosamine by marine bacteria:production and process optimization[J].World Journal of Microbiology&Biotechnology,2012,28(10):2945-2962.
[27]单秋实.壳聚糖酶高产菌株的筛选及酶学性质的研究[D].长春:吉林大学,2015.
[28]Liang D C,Liu W G,Zuo A J,et al.Pre-deliver chitosanase to cells:a novel strategy to improve gene expression by endocellular degradation-induced vector unpacking[J].International Journal of Pharmaceutics,2006,314(1):63-71.
[29]Kouzai Y,Mochizuki S,Saito A,et al.Expression of a bacterial chitosanase in rice plants improves disease resistance tothericeblastfungusMagnaporthe oryzae[J].Plant Cell Reports,2012,31(4):629-636.
[30]Nidheesh T,Suresh P V.Recent developments in chitosanase research and its biotechnological applications:a review[J].Food Chemistry,2013,150:392-399.
[2]SomashekarD, JosephR. Chitosanases-propertiesand applications:a review[J].Bioresource Technology,1996,55(1):35-45.
[3]Gao X A,Ju W T,Jung W J,et al.Purification and characterization of chitosanase from Bacillus cereus D-11[J].Carbohydrate Polymers,2007,72(3):513-520.
[4]Wang S L,Chao C H,Liang T W,et al.Purification and characterization of protease and chitinase from Bacillus cereus TKU006and conversion of marine wastes by these enzymes[J].Marine Biotechnology,2009,11(3):334-344.
[5]Zitouni M,Fortin M,Scheerle R K,et al.Biochemical and molecular characterization of a thermostable chitosanase produced by the strain Paenibacillus sp.1794newly isolated from compost[J].Applied Microbiology and Biotechnology,2013,97(13):5801-5813.
[6]Wang S L,Peng J H,Liang T W,et al.Purification and characterization of a chitosanase from Serratia marcescens TKU011[J].Carbohydrate Research,2008b,343(8):1316-1323.
[7]Liu Y L,Jiang S,Ke Z M,et al.Recombinant expression of a chitosanase and its application in chitosan oligosaccharide production[J].Carbohydrate Research,2009,344(6):815-819.
[8]Johnsen M G,Hansen O C,Stougaard P.Isolation,characterization and heterologous expression of a novel chitosanase from Janthino bacterium sp.strain 4239[J].Microbial Cell Factories,2010,9(1):1-5.
[9]Wang S L,Tseng W N,Liang T W.Biodegradation of shellfish wastes and production of chitosanases by a squid pen-assimilating bacterium,Acinetobacter calcoaceticus TKU024[J].Biodegradation,2011,22(5):939-948.
[10]Jiang X,Chen D,Chen L,et al.Purification,characterization and action mode of a chitosanase from Streptomyces roseolus induced by chitin[J].Carbohydrate Research,2012,355(7):40-44.
[11]Zhang H,Sang Q,Zhang W.Statistical optimization of chitosanase production by Aspergillus sp. QD-2 in submerged fermentation[J].Annals of Microbiology,2012,62(1):193-201.
[12]Wang J,Zhou W,Yuan H,et al.Characterization of a novel fungal chitosanase Csn2from Gongronella sp.JG[J].Carbohydrate Research,2008,343(15):2583-2588.
[13]Zhou W,Yuan H,Wang J,et al.Production,purification andcharacterizationofchitosanaseproducedby Gongronellasp.JG[J].Letters in Applied Microbiology,2008,46(1):49-54.
[14]Da Silva L C,Honorato T L,Franco T T,et al.Optimization of chitosanase production by Trichoderma koningii sp.under solid-state fermentation[J].Food and Bioprocess Technology,2012(5):1564-1572.
[15]Gupta V,Prasanna R,Srivastava A K,et al.Purification and characterization of a novel antifungal endo-type chitosanase from Anabaena fertilissima[J].Annals of Microbiology,2012,62(3):1089-1098.
[16]Hsu S K,Chung Y C,Chang C T,et al.Purification and characterization of two chitosanase isoforms from the sheaths of bamboo shoots[J].Journal of Agricultural and Food Chemistry,2012,60(2):649-657.
[17]Fukamizo T,Fleury A C N,Mitsutomi M,et al.Exo-β-Dglucosaminidase from Amycolatopsis orientalis:catalytic residues,sugar recognition specificity, kinetics, and synergism[J].Glycobiology,2006,16(11):1064-1072.
[18]Chen X M,Zhai C,Kang L,et al.High-level expression and characterization of a highly thermostable chitosanase from Aspergillus fumigatus in Pichia pastoris[J].Biotechnology Letters,2012,34(4):689-694.
[19]Marcotte E M,Monzingo A F,Ernst S R.X-ray structure of an anti-fungal chitosanase fromStreptomyces N174[J].Nature Structural Biology,1996,3(2):155-162.
[20]Saito J,Kita A,Higuchi Y,et al.Crystal structure of chitosanase from Bacillus circulans MH-K1at 1.6-AA resolution and its substrate recognition mechanism[J].Journal of Biological Chemistry,1999,274(43):30818-30825.
[21]Liu G L,Li Y,Zhou H X,et al.Over-expression of a bacterial chitosanase gene in Yarrowia lipolytica and chitosan hydrolysis by the recombinant chitosanase[J].Journal of Molecular Catalysis B:Enzymatic,2012,83(11):100-107.
[22]Liu H,Bao X.Characterization of a chitosanase from Fusarium solani and its expression in an industrial strain of Saccharomyces cerevisiae[J]. Acta Microbiologica Sinica,2009,49(12):1607-1612.
[23]Kilani-Feki O,Frikha F,Zouari I,et al. Heterologous expression and secretion of an antifungal Bacillus subtilis chitosanase(CSNV26)in Escherichia coli[J].Bioprocess and Biosystems Engineering,2013,36(7):1-8.
[24]Zhu X F,Tan H Q,Zhu C,et al.Cloning and overexpression of a new chitosanase gene fromPenicilliumsp.D-1[J].AMB Express,2012,2(1):1-8.
[25]Ming M,Kuroiwa T,Ichikawa S,et al.Production of chitosanoligosaccharidesbychitosanasedirectly immobilized on an agar gelcoated multidisk impeller[J].Biochemical Engineering Journal,2006,28(3):289-294.
[26]Suresh P V.Biodegradation of shrimp processing bio-waste and concomitant production of chitinase enzyme and N-acetyl-D-glucosamine by marine bacteria:production and process optimization[J].World Journal of Microbiology&Biotechnology,2012,28(10):2945-2962.
[27]单秋实.壳聚糖酶高产菌株的筛选及酶学性质的研究[D].长春:吉林大学,2015.
[28]Liang D C,Liu W G,Zuo A J,et al.Pre-deliver chitosanase to cells:a novel strategy to improve gene expression by endocellular degradation-induced vector unpacking[J].International Journal of Pharmaceutics,2006,314(1):63-71.
[29]Kouzai Y,Mochizuki S,Saito A,et al.Expression of a bacterial chitosanase in rice plants improves disease resistance tothericeblastfungusMagnaporthe oryzae[J].Plant Cell Reports,2012,31(4):629-636.
[30]Nidheesh T,Suresh P V.Recent developments in chitosanase research and its biotechnological applications:a review[J].Food Chemistry,2013,150:392-399.