利用Folin-Ciocalteu比色法测定石榴花多酚含量条件的系统研究
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摘要
优化Folin-Ciocalteu比色法测定石榴花提取物中多酚含量的条件,并对该方法进行系统评价。以没食子酸为标准物质,确定测定波长后系统考察Folin-Ciocalteu试剂(FC试剂)浓度、Na2CO3试剂浓度、反应时间、反应温度等因素对测定结果的影响,并对该方法的稳定性、精密度、回收率等进行试验。研究表明,石榴花提取物多酚含量测定的适宜条件为:1 m L样品液、5 m L 40%FC试剂、4 m L 10%Na_2CO_3试剂、25~℃避光反应100 min,测定波长为750 nm,该方法的稳定性、精密度、回收率均较好。
The optimum conditions for the measurement of polyphenol in pomegranate flowers by Folin-Ciocalteu colorimetry were optimized,and the method was evaluated systematically. The effects of the concentration of FC reagent,concentration of Na2 CO3 reagent,reaction time and reaction temperature on the results were studied by using gallic acid as the reference material. The stability,precision and recovery rate of the method were tested. It showed that the optimum conditions for the measurement of polyphenols in pomegranate flower extracts were:1 m L sample solution,5 m L 40 % FC reagent,4 m L 10 % Na_2 CO_3 reagent,which were reacted at 25~℃ for 100 min,and the measurement wavelength was 750 nm. The stability,precision and recovery of the method are good.
The optimum conditions for the measurement of polyphenol in pomegranate flowers by Folin-Ciocalteu colorimetry were optimized,and the method was evaluated systematically. The effects of the concentration of FC reagent,concentration of Na2 CO3 reagent,reaction time and reaction temperature on the results were studied by using gallic acid as the reference material. The stability,precision and recovery rate of the method were tested. It showed that the optimum conditions for the measurement of polyphenols in pomegranate flower extracts were:1 m L sample solution,5 m L 40 % FC reagent,4 m L 10 % Na_2 CO_3 reagent,which were reacted at 25~℃ for 100 min,and the measurement wavelength was 750 nm. The stability,precision and recovery of the method are good.
引文
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[12]朱彩平,夏德水.Folin-Ciocalteu比色法测定石榴皮提取物中的多酚[J].光谱实验室,2012,29(3):1356-1360
[2]Lei Wang,Jeffrey Ho,Carlotta Glackin,et al.Specific Pomegranate Juice Components as Potential Inhibitors of Prostate Cancer Metastasis[J].Translational oncology,2012,5(5):344-355
[3]Hamid Reza Rahimi,Mohammad Arastoo,Seyed Nasser Ostad.A comprehensive review of puncia granatum(pomegranate)properties in toxicological,pharmacological,cellular and molecular biology researches[J].Iranian Journal of Pharmaceutical Research,2012,11(2):385-400
[4]Eliana Harue Endo,Tania Ueda-Nakamura,Celso Vataru Nakamura,et al.Activity of spray-dried microparticles containing pomegranate peel extract against Candida albicans[J].Molecules,2012,17:10094-10107
[5]Iqbal Ahmad.Bioactive compounds from Punica granatum,Curcuma longa and Zingiber officinale and their therapeutic potential[J].Drugs of the Future,2008,33(4):329-346
[6]Aide Zarfeshany,Sedigheh Asgary,Shaghayegh Haghjoo Javanmard.Potent health effects of pomegranate[J].Advanced Biomedical Research,2014,3:100-120
[7]张立华,董业成,张元湖.大孔吸附树脂纯化石榴花多酚的工艺[J].江苏农业科学,2015,43(10):359-362
[8]Bektas N,Ozturk N.Antioxidant activity of Punica granatum(Pomegranate)flowers[J].Toxicology Letters,2007,172(Supp.):62
[9]Huang T H W,Peng G,Kota B P,et al.Pomegranate flower improves cardiac lipid metabolism in a diabetic rat model:role of lowering circulating lipids[J].British Journal of Pharmacology,2005(145):767-774
[10]Albrecht Martin,Jiang Wenguo,Kumi-Diaka James,et al.Pomegranate extracts potently suppress proliferation,xenograft growth,and invasion of human prostate cancer cells[J].Journal of medicinal food,2004,7(3):274-283
[11]中华全国供销合作总社杭州茶叶研究院.GB/T 8313-2008茶叶中茶多酚和儿茶素类含量的检测方法[S].北京:标准出版社,2008
[12]朱彩平,夏德水.Folin-Ciocalteu比色法测定石榴皮提取物中的多酚[J].光谱实验室,2012,29(3):1356-1360