MR报告基因及其在肿瘤诊治中应用的研究进展
摘要
报告基因显像技术是分子影像学中重要的成像技术,其将报告基因与报告探针结合在一起,通过探针的聚集显像报告基因产物的活性水平,从而间接提供报告基因表达水平及驱动报告基因表达的内源性信号或转录因子水平的信息,有望成为一门从基础研究到临床应用的学科,在疾病的早期发现、精确定位、准确定量、基因治疗的效能评估等方面发挥重要作用,为临床提供更多有价值的信息。本文就MR报告基因分类及其在肿瘤诊治的应用等作一综述。
引文
[1]张龙江,祁吉.分子影像学报告基因显像的研究进展[J].中国医学影像技术,2005,21(11):1 772-1 775.
[2]杨燕,张冬.MR报告基因成像研究现状[J].中华放射学杂志,2014,48(2):171-174.
[3]Sengupta A,Quiaoit K,Thompson RT,et al.Biophysical features of Mag A expression in mammalian cells:implications for MRI contrast[J].Front Microbiol,2014,5:1-9.
[4]贺小娅,蔡金华,秦勇,等.磁共振报告基因FTH1慢病毒载体构建及其在人神经母细胞瘤细胞中的表达[J].第三军医大学学报,2014,36(23):2 338-2 342.
[5]Weissleder R,Simonova M,Bogdanova A,et al.MR imaging and scintigraphy of gene expression through melanin induction[J].Radiology,1997,204(2):425-429.
[6]Qin C,Cheng K,Chen K,et al.Tyrosinase as a multifunctional reporter gene for Photoacoustic/MRI/PET triple modality molecular imaging[J].Sci Rep,2013,(3):1 490-1 497.
[7]Alfke H,Stppler H,Nocken F,et al.In vitro MR imaging of regulated gene expression[J].Radiology,2003,228(2):488-492.
[8]Hundt W,Schink C,Steinbach SA,et al.Effect of antiangiogenic therapy on luciferase activity in a cytomegalovirus-or HSP70-promoter-transfected M21 tumor model[J].J Biomed Opt,2012,17(6):065001-1-06500-7.
[9]Zhang XY,Robledo BN,Harris SS,et al.A bacterial gene,MMS6,as a new reporter gene for magnetic resonance imaging of mammalian cells[J].Mol Imaging,2014,14:H2.
[10]Sun YJ,Kim HS,Park J,et al.MRI of breast tumor initiating cells using the extra domain-B of fibronectin targeting nanoparticles[J].Theranostics,2014,4(8):845-857.
[11]张业森,郭永坤,姚慧,等.转铁蛋白受体基因在大鼠脑内表达成像的研究[J].中华实验外科杂志,2013,30(12):2 605.
[12]王凯.转铁蛋白受体报告基因MR分子成像监测内皮抑素基因表达及治疗效果[D].哈尔滨:哈尔滨医科大学,2009.
[13]周金桥,孙剑瑞,宋来君,等.组织蛋白酶D酶原和转铁蛋白受体在人脑胶质瘤中的表达[J].中国神经精神疾病杂志,2011,37(5):311-313.
[14]胡慧慧,孟倩,张曼.结直肠癌患者血清中转甲状腺素蛋白的表达与肿瘤转移的相关性[J].标记免疫分析与临床,2013,20(6):378-380.
[15]张斌,杨卉,肖煜东,等.人转铁蛋白受体基因载体的构建与鉴定及在Hela细胞中的表达[J].中华消化病与影像杂志:电子版,2013,3(2):28-33.
[16]Ichikawa T,Hgemann D,Saeki Y,et al.MRI of transgene expression:correlation to therapeutic gene expression[J].Neoplasia,2002,4(6):523-530.
[17]江新青,陈亮,吴红珍,等.经腺病毒介导的胞嘧啶脱氨酶-脱氧胸苷激酶基因治疗裸鼠Bel7402肝癌的MR扩散加权成像特点初探[J].中华放射学杂志,2012,46(8):738-741.
[18]黄建华,王瑶,李崇辉,等.19F磁共振波谱分析CD基因在人结肠癌细胞的表达[J].波谱学杂志,2004,21(3):289-294.
[19]Forbes SC,Bish LT,Ye F,et al.Gene transfer of arginine kinase to skeletal muscle using adeno-associated virus[J].Gene Ther,2014,21(4):387-392.
[20]Walter G,Barton ER,Sweeney HL.Noninvasive measurement of gene expression in skeletal muscle[J].Proc Natl Acad Sci U S A,2000,97(10):5 151-5 155.
[21]Hundt W,Steinbach S,Burbelko M,et al.Induction of luciferase activity under the control of an hsp70 promoter using high-intensity focused ultrasound:combination of bioluminescence and MRI imaging in three different tumour models[J].Technol Cancer Res Treat,2011,10(2):197-210.
[22]Thomas C,Gustafsson JA.Targeting PES1 for restoring the ERα/ERβratio in breast cancer[J].J Clin Invest,2012,122(8):2 771-2 773.
[23]江絮萍,叶泓.PES1在卵巢癌中的表达及其对内皮生长因子的影响[J].重庆医学,2013,42(27):3 211-3 213.
[24]陈昆,刘金龙,杨超,等.人颅内星形细胞瘤PES1的表达及与患者生存的关系[J].中华显微外科杂志,2009,32(6):512-514.
[2]杨燕,张冬.MR报告基因成像研究现状[J].中华放射学杂志,2014,48(2):171-174.
[3]Sengupta A,Quiaoit K,Thompson RT,et al.Biophysical features of Mag A expression in mammalian cells:implications for MRI contrast[J].Front Microbiol,2014,5:1-9.
[4]贺小娅,蔡金华,秦勇,等.磁共振报告基因FTH1慢病毒载体构建及其在人神经母细胞瘤细胞中的表达[J].第三军医大学学报,2014,36(23):2 338-2 342.
[5]Weissleder R,Simonova M,Bogdanova A,et al.MR imaging and scintigraphy of gene expression through melanin induction[J].Radiology,1997,204(2):425-429.
[6]Qin C,Cheng K,Chen K,et al.Tyrosinase as a multifunctional reporter gene for Photoacoustic/MRI/PET triple modality molecular imaging[J].Sci Rep,2013,(3):1 490-1 497.
[7]Alfke H,Stppler H,Nocken F,et al.In vitro MR imaging of regulated gene expression[J].Radiology,2003,228(2):488-492.
[8]Hundt W,Schink C,Steinbach SA,et al.Effect of antiangiogenic therapy on luciferase activity in a cytomegalovirus-or HSP70-promoter-transfected M21 tumor model[J].J Biomed Opt,2012,17(6):065001-1-06500-7.
[9]Zhang XY,Robledo BN,Harris SS,et al.A bacterial gene,MMS6,as a new reporter gene for magnetic resonance imaging of mammalian cells[J].Mol Imaging,2014,14:H2.
[10]Sun YJ,Kim HS,Park J,et al.MRI of breast tumor initiating cells using the extra domain-B of fibronectin targeting nanoparticles[J].Theranostics,2014,4(8):845-857.
[11]张业森,郭永坤,姚慧,等.转铁蛋白受体基因在大鼠脑内表达成像的研究[J].中华实验外科杂志,2013,30(12):2 605.
[12]王凯.转铁蛋白受体报告基因MR分子成像监测内皮抑素基因表达及治疗效果[D].哈尔滨:哈尔滨医科大学,2009.
[13]周金桥,孙剑瑞,宋来君,等.组织蛋白酶D酶原和转铁蛋白受体在人脑胶质瘤中的表达[J].中国神经精神疾病杂志,2011,37(5):311-313.
[14]胡慧慧,孟倩,张曼.结直肠癌患者血清中转甲状腺素蛋白的表达与肿瘤转移的相关性[J].标记免疫分析与临床,2013,20(6):378-380.
[15]张斌,杨卉,肖煜东,等.人转铁蛋白受体基因载体的构建与鉴定及在Hela细胞中的表达[J].中华消化病与影像杂志:电子版,2013,3(2):28-33.
[16]Ichikawa T,Hgemann D,Saeki Y,et al.MRI of transgene expression:correlation to therapeutic gene expression[J].Neoplasia,2002,4(6):523-530.
[17]江新青,陈亮,吴红珍,等.经腺病毒介导的胞嘧啶脱氨酶-脱氧胸苷激酶基因治疗裸鼠Bel7402肝癌的MR扩散加权成像特点初探[J].中华放射学杂志,2012,46(8):738-741.
[18]黄建华,王瑶,李崇辉,等.19F磁共振波谱分析CD基因在人结肠癌细胞的表达[J].波谱学杂志,2004,21(3):289-294.
[19]Forbes SC,Bish LT,Ye F,et al.Gene transfer of arginine kinase to skeletal muscle using adeno-associated virus[J].Gene Ther,2014,21(4):387-392.
[20]Walter G,Barton ER,Sweeney HL.Noninvasive measurement of gene expression in skeletal muscle[J].Proc Natl Acad Sci U S A,2000,97(10):5 151-5 155.
[21]Hundt W,Steinbach S,Burbelko M,et al.Induction of luciferase activity under the control of an hsp70 promoter using high-intensity focused ultrasound:combination of bioluminescence and MRI imaging in three different tumour models[J].Technol Cancer Res Treat,2011,10(2):197-210.
[22]Thomas C,Gustafsson JA.Targeting PES1 for restoring the ERα/ERβratio in breast cancer[J].J Clin Invest,2012,122(8):2 771-2 773.
[23]江絮萍,叶泓.PES1在卵巢癌中的表达及其对内皮生长因子的影响[J].重庆医学,2013,42(27):3 211-3 213.
[24]陈昆,刘金龙,杨超,等.人颅内星形细胞瘤PES1的表达及与患者生存的关系[J].中华显微外科杂志,2009,32(6):512-514.