基于SSR标记的我国咖啡种质资源遗传多样性分析及指纹图谱构建
|
摘要
为分析我国咖啡种质遗传背景,提高咖啡资源保护与利用效率,利用31个SSR分子标记对我国主要的92份咖啡种质进行遗传多样性检测。结果表明, 92份咖啡种质遗传相似系数变幅为0.666～0.997,平均为0.85,其中小粒种咖啡种内平均遗传相似系数为0.923。在遗传多样性分析基础上,利用5个SSR标记构建了25个中粒种咖啡品系的DNA指纹图谱。研究表明,我国咖啡种质资源遗传基础非常狭窄,利用现有咖啡资源开展品种选育潜力有限,应加强对野生特异种质的引种与发掘利用。
In order to analyze the genetic basis of coffee germplasm in China and consequently improve the efficiency of the protection and utilization of the coffee germplasm resources, the genetic diversity of92 accessions of major coffee germplasm in China was detected by using 31 SSR molecular markers. The results showed that the genetic similarity coefficient of the 92 accessions of coffee germplasm varied from0.666 to 0.997, with an average of 0.85. The average genetic similarity coefficient of Arabica coffee was0.923. Based on the genetic diversity analysis, DNA fingerprinting of 25 Robusta coffee clones was conducted using by using 5 SSR markers. The results showed that genetic diversity basis of coffee germplasm resources in China was very low, limiting the potential of variety development by using the existing coffee germplasm resources. The introduction and exploration of specific wild coffee germplasm should be highly emphasized.
In order to analyze the genetic basis of coffee germplasm in China and consequently improve the efficiency of the protection and utilization of the coffee germplasm resources, the genetic diversity of92 accessions of major coffee germplasm in China was detected by using 31 SSR molecular markers. The results showed that the genetic similarity coefficient of the 92 accessions of coffee germplasm varied from0.666 to 0.997, with an average of 0.85. The average genetic similarity coefficient of Arabica coffee was0.923. Based on the genetic diversity analysis, DNA fingerprinting of 25 Robusta coffee clones was conducted using by using 5 SSR markers. The results showed that genetic diversity basis of coffee germplasm resources in China was very low, limiting the potential of variety development by using the existing coffee germplasm resources. The introduction and exploration of specific wild coffee germplasm should be highly emphasized.
引文
[1]Davis A P,Govaerts R,Bridson D M,et al.An annotated taxonomic conspectus of the genus Coffea(Rubiaceae)[J].Botanical Journal of the Linnean Society,2010,152(4):465-512.
[2]周华,张洪波,夏红云,等.咖啡种质资源遗传多样性研究[J].热带农业,2015(5):23-27.
[3]黄丽芳,董云萍,王晓阳,等.利用RAPD标记分析咖啡种质资源的遗传多样性[J].作物学报,2014,35(12):2 313-2 319.
[4]闫林,黄丽芳,王晓阳,等.一种利用ISSR指纹图谱鉴定咖啡种质资源的方法[Z].中国.201510556607.2.2018.
[5]Hoang Thi Hue,La Tuan Nghia,Hoang Tuyet Minh,et al.Evaluation of genetic diversity of local-colored rice landraces using SSR markers[J].International Letters of Natural Sciences,2018,67:24-34.
[6]Meng Yusha,Liu Qingchang.SSR fingerprinting of203 sweetpotato[Ipomoea batatas(L.)Lam.]varieties[J].Journal of Integrative Agriculture,2018,17(1):86-93.
[7]Masahiro Kishine,Katsuji Tsutsumi,Kazumi Kitta.A set of tetra-nucleotide core motif SSR markers for efficient identification of potato(Solanum tuberosum)cultivars[J].Breeding Science,2017,67(5):544-547.
[8]Anchal Bisen,Dhirendra Khare,Priya Nair,et al.SSR analysis of 38 genotypes of soybean[Glycine Max(L.)Merr.]genetic diversity in India[J].Physiol Mol Biol Plants,2015,21(1):109-115.
[9]Jhansi G,Surender M,Aparnna,et al.DNA finger printing of maize hybrids and development of molecular ID's using SSR markers their parental lines using simple sequence repeat markers[J].Research Journal of Agricultural Science,2016,6(1):43-47.
[10]Elisa S N,Edila V de R Von Pinho,Maria G G,et al.Development of microsatellite markers for identifying Brazilian Coffea arabica varieties[J].Genetics and Molecular Biology,2010,33(3):507-514.
[11]Poncet V,Dufour M,Hamon P,et al.Development of genomic microsatellite markers in coffea canephora and their transferability to other coffee species[J].Genome,2007,50(12):1 156-1 161.
[12]Hendre P S,Phanindranath R,Annapurna V,et al.Development of new genomic microsatellite markers from robusta coffee(Coffea canephora Pieere ex A.Froehner)showing broad cross-species transferability and utility in genetic studies[J].BMC Plant Biology,2008,8(1):51.
[13]Cubry P,Musoli P,Legnate H,et al.Diversity in coffee assessed with SSR markers:structure of the genus Coffea and perspectives for breeding[J].Genome,2008,51(1):50-63.
[14]Musoli P,Cubry P,Aluka P,et al.Genetic differentiation of wild and cultivated populations:diversity of Coffea canephora Pierre in Uganda.[J].Génome,2011,52(7):634.
[15]Poncet V,Hamon P,Minier J,et al.SSR cross-amplification and variation within coffe trees(Coffea spp.)[J].Genome,2004,47(6):1 071-1 081.
[16]Baruah A,Naik V,Hendre P S,et al.Isolation and characterization of nine microsatellite markers from Coffea ababica L.,showing wide cross-species amplifications[J].Molecular Ecology Notes,2003,3(4):647-650.
[17]Silvestrini M,Junqueira M G,Andréa C.Favarin,et al.Genetic diversity and structure of Ethiopian,Yemen and Brazilian Coffea arabicaL.accessions using microsatellites markers[J].Genetic Resources and Crop Evolution,2007,54(6):1 367-1 379.
[18]Bertrand B,Alpizar E,Lara L,et al.Performance of coffea Arabica F1 hybrids in agroforestry and full-sun cropping systems in comparison with American pure line cultivars[J].Euphytica,2011,181(2):147-158.
[19]Habtamu Gebreselassie,Gizachew Atinafu,Meseret Degefa,et al.Arabica coffee(Coffea Arabica L.)hybrid genotypes evaluation for growth characteristics and yield performance under southern Ethiopian growing condition[J].Acad.Res.J.Agri.Sci,2018,6(2):89-96.
[20]王晓阳,董云萍,黄丽芳,等.利用SSR分子标记快速鉴别中粒种与小粒种咖啡[J].热带作物学报,2014,35(4):622-627.
[2]周华,张洪波,夏红云,等.咖啡种质资源遗传多样性研究[J].热带农业,2015(5):23-27.
[3]黄丽芳,董云萍,王晓阳,等.利用RAPD标记分析咖啡种质资源的遗传多样性[J].作物学报,2014,35(12):2 313-2 319.
[4]闫林,黄丽芳,王晓阳,等.一种利用ISSR指纹图谱鉴定咖啡种质资源的方法[Z].中国.201510556607.2.2018.
[5]Hoang Thi Hue,La Tuan Nghia,Hoang Tuyet Minh,et al.Evaluation of genetic diversity of local-colored rice landraces using SSR markers[J].International Letters of Natural Sciences,2018,67:24-34.
[6]Meng Yusha,Liu Qingchang.SSR fingerprinting of203 sweetpotato[Ipomoea batatas(L.)Lam.]varieties[J].Journal of Integrative Agriculture,2018,17(1):86-93.
[7]Masahiro Kishine,Katsuji Tsutsumi,Kazumi Kitta.A set of tetra-nucleotide core motif SSR markers for efficient identification of potato(Solanum tuberosum)cultivars[J].Breeding Science,2017,67(5):544-547.
[8]Anchal Bisen,Dhirendra Khare,Priya Nair,et al.SSR analysis of 38 genotypes of soybean[Glycine Max(L.)Merr.]genetic diversity in India[J].Physiol Mol Biol Plants,2015,21(1):109-115.
[9]Jhansi G,Surender M,Aparnna,et al.DNA finger printing of maize hybrids and development of molecular ID's using SSR markers their parental lines using simple sequence repeat markers[J].Research Journal of Agricultural Science,2016,6(1):43-47.
[10]Elisa S N,Edila V de R Von Pinho,Maria G G,et al.Development of microsatellite markers for identifying Brazilian Coffea arabica varieties[J].Genetics and Molecular Biology,2010,33(3):507-514.
[11]Poncet V,Dufour M,Hamon P,et al.Development of genomic microsatellite markers in coffea canephora and their transferability to other coffee species[J].Genome,2007,50(12):1 156-1 161.
[12]Hendre P S,Phanindranath R,Annapurna V,et al.Development of new genomic microsatellite markers from robusta coffee(Coffea canephora Pieere ex A.Froehner)showing broad cross-species transferability and utility in genetic studies[J].BMC Plant Biology,2008,8(1):51.
[13]Cubry P,Musoli P,Legnate H,et al.Diversity in coffee assessed with SSR markers:structure of the genus Coffea and perspectives for breeding[J].Genome,2008,51(1):50-63.
[14]Musoli P,Cubry P,Aluka P,et al.Genetic differentiation of wild and cultivated populations:diversity of Coffea canephora Pierre in Uganda.[J].Génome,2011,52(7):634.
[15]Poncet V,Hamon P,Minier J,et al.SSR cross-amplification and variation within coffe trees(Coffea spp.)[J].Genome,2004,47(6):1 071-1 081.
[16]Baruah A,Naik V,Hendre P S,et al.Isolation and characterization of nine microsatellite markers from Coffea ababica L.,showing wide cross-species amplifications[J].Molecular Ecology Notes,2003,3(4):647-650.
[17]Silvestrini M,Junqueira M G,Andréa C.Favarin,et al.Genetic diversity and structure of Ethiopian,Yemen and Brazilian Coffea arabicaL.accessions using microsatellites markers[J].Genetic Resources and Crop Evolution,2007,54(6):1 367-1 379.
[18]Bertrand B,Alpizar E,Lara L,et al.Performance of coffea Arabica F1 hybrids in agroforestry and full-sun cropping systems in comparison with American pure line cultivars[J].Euphytica,2011,181(2):147-158.
[19]Habtamu Gebreselassie,Gizachew Atinafu,Meseret Degefa,et al.Arabica coffee(Coffea Arabica L.)hybrid genotypes evaluation for growth characteristics and yield performance under southern Ethiopian growing condition[J].Acad.Res.J.Agri.Sci,2018,6(2):89-96.
[20]王晓阳,董云萍,黄丽芳,等.利用SSR分子标记快速鉴别中粒种与小粒种咖啡[J].热带作物学报,2014,35(4):622-627.