21份葡萄砧木品种资源耐盐性鉴定
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摘要
为了准确鉴定21份葡萄砧木品种资源的耐盐性。采用营养钵基部盐液浸泡法,设置100 mmol/L盐处理(以不加盐作为对照),以盐害指数为指标,通过聚类分析对21份葡萄砧木进行了耐盐性鉴定。结果表明:耐盐力强的‘Salt creek’和‘Dogridge’来自香槟尼葡萄,‘洛特’来自沙地葡萄,‘101-14’来自河岸葡萄作为母本的杂交组合,而耐盐力弱的是‘5BB’‘188-08’‘420A’‘140R’‘1103P’‘SO4’均来自冬葡萄作为母本的杂交组合。因此,耐盐砧木育种中,可选择耐盐性强的香槟尼、沙地葡萄和河岸葡萄作为母本进行杂交育种。
In order to accurately identify salt tolerance of twenty-one grape rootstock resources, twenty-one grape rootstocks were planted in pots whose base soaking in salt solution with the concentration gradient of 100 mmol/L NaCl.According to salt injury index, the salt tolerance of rootstocks were ascertained by cluster analysis.The results showed that ‘salt creek',‘Dogridge',Rupestris du Lot and ‘101-14' with strong salt tolerance all came from the cross combination of V.champini,V.rupetris,V.riparia as female parent, separately.However, ‘5 BB'‘188-08'‘420 A'‘140 R'‘1103 P' and ‘SO4' with weak salt tolerance all came from the cross combination of V.Berlandier as female parent.In the breeding of salt-tolerant rootstock, V.champini,V.rupetris and V.riparia with strong salt tolerance could be selected as female parent for cross breeding.
In order to accurately identify salt tolerance of twenty-one grape rootstock resources, twenty-one grape rootstocks were planted in pots whose base soaking in salt solution with the concentration gradient of 100 mmol/L NaCl.According to salt injury index, the salt tolerance of rootstocks were ascertained by cluster analysis.The results showed that ‘salt creek',‘Dogridge',Rupestris du Lot and ‘101-14' with strong salt tolerance all came from the cross combination of V.champini,V.rupetris,V.riparia as female parent, separately.However, ‘5 BB'‘188-08'‘420 A'‘140 R'‘1103 P' and ‘SO4' with weak salt tolerance all came from the cross combination of V.Berlandier as female parent.In the breeding of salt-tolerant rootstock, V.champini,V.rupetris and V.riparia with strong salt tolerance could be selected as female parent for cross breeding.
引文
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[3] 刘会超,贾文庆.盐胁迫对白三叶茎的POD、CAT的影响研究[J].吉林农业科学,2009,34(1):43-46. LIU H CH,JIA W Q.Studies on POD,CAT of white clover stems under salt stress[J].Journal of Jilin Agricultural Sciences,2009,34(1):43-46.
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[7] 袁军伟,郭紫娟,刘长江,等.营养钵基部浸泡盐液法鉴定葡萄砧木的耐盐性及其生理响应[J].西南大学学报(自然科学版),2015,37(7):44-51. YUAN J W,GUO Z J,LIU CH J,et al.Evaluating the salt tolerance of grape rootstocks by base soaking of the plants with salt solution[J].Journal of Southwest University(Natural Science Edition),2015,37(7):44-51.
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[9] GONG H J,DEIDRE B,PETER C,et al.Contrast in chloride exclusion between two grapevine genotypes and its variation in their hybrid progeny[J].Journal of Experimental Botany,2011,62(3):989-999.
[10] WALKER R R,BLACKMORE D H,CLNGELEFFER P R,et al.Rootstock effects on salt tolerance of irrigated field-grown grapevines(Vitis vinifera L.cv.sultana).I.ion concentration in leaves and juice[J].Australian Journal of Agricultural Research,2002,8(1):3-14.
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[12] DOWNTON W J S,LOVEYS B R.Abscissic acid content and osmotic relations of salt-stressed grape vine leaves[J].Functional Plant Biology,1981,8(5):443-452.
[13] HATAMI E,ASHARI M E,JAVADI T.Effect of salinity on some gas exchange characteristics of grape(Vitis vinifera) cultivars[J].International Journal of Agriculture and Biology,2010,12(2):1560-8530.
[14] 周万海.葡萄砧木耐盐性及砧-穗特性的研究[D].兰州:甘肃农业大学,2005. ZHOU W H.Studies on salt tolerance of grape rootstock and characteristics of rootstock-scion interaction[D].Lanzhou:Gansu Agricultural University,2005.
[15] ALPER D,NURAY M,MüFTüOGLU,H,et al.Determination of salt tolerance of some American grape rootstocks[J].Bangladesh Journal of Botany,2006,35(2):143-150.
[16] 付晴晴,孙鲁龙,翟衡,等.葡萄种间杂交砧木育种F1代植株耐盐性分析[J].植物学报,2017,52(6):733-743. FU Q Q,SUN L L,ZHAI H,et al.Salt tolerant evaluation of F1-generation hybrids in grape[J].Chinese Bulletin of Botany,2017,52(6):733-743.
[17] 陈继峰.葡萄砧木品种的研究现状与展望[J].果树科学,2000,17(2):138-146. CHEN J F.The status of research on grape rootstock varieties and its prospect[J].Journal of Fruit Science,2000,17(2):138-146.
[18] 袁军伟,马爱红,郭紫娟,等.葡萄砧木抗逆性鉴定方法探讨[J].中外葡萄与葡萄酒,2011(11):66-75. YUAN J W,MA A H,GUO Z J,et al.Study on identification method of grape stock resistance[J].Sino-Overseas Grapevine and Wine,2011(11):66-75.
[19] 刘崇怀.葡萄种质资源描述规范和数据标准[M].北京:中国农业出版社,2006. LIU CH H.Description Standard and Data Standard of Grape Germplasm Resources[M].Beijing:China Agriculture Press,2006.
[20] 李会云,郭修武.盐胁迫对葡萄砧木叶片保护酶活性和丙二醛含量的影响[J].果树学报,2008,25(2):240-243. LI H Y,GUO X W.Influence of NaCl on activities of protective enzymes and MDA content in grape rootstock leaves[J].Journal of Fruit Science,2008,25(2):240-243.
[21] BELEW D,ASTATKIE T,MOKASHI M N,et al.Effects of salinity and mycorrhizal inoculation(Glomus fasciculatum) on growth responses of grape rootstocks(Vitis spp.)[J].South African Journal for Enology and Viticulture,2010,31(2):82-88.
[22] SOUTHEY J M,JOOSTE J H.Physiological response of Vitis vinifera L.cv.chenin blanc grafted onto different rootstocks on a relatively saline soil[J].South African Journal for Enology and Viticulture,1992,13(1):10-22.
[23] THOMAS W A.Development of a repeatable,low-cost,high-through put and precise salt tolerance assay for grapevines[D].Davis(USA):University of California:2011.
[24] 樊秀彩,刘崇怀,潘兴,等.水培条件下葡萄砧木对氯化钠的耐性鉴定[J].果树学报,2004,21(2):128-129. FAN X C,LIU CH H,PAN X,et al.Evaluation of salt tolerance of grape rootstocks under hydroponic culture conditions[J].Journal of Fruit Science,2004,21(2):128-129.
[25] 秦玲,齐艳玲,秦子禹,等.葡萄耐盐生理生化特性研究进展[J].河北科技师范学院学报,2011,25(3):75-80. QIN L,QI Y L,QIN Z Y,et al.Advances on physiological and biochemical characteristics of grape under salt stress[J].Journal of Hebei Normal University of Science and Technology,2011,25(3):75-80.
[2] BAAUIS J,LEGAZ F,PRIMO M E.Effect of salinity on uptake and distribution of chloride and sodium in some citrus scion-rootstock combinations[J].Journal of Pomology and Horticultural Science,2015,65(6):715-724.
[3] 刘会超,贾文庆.盐胁迫对白三叶茎的POD、CAT的影响研究[J].吉林农业科学,2009,34(1):43-46. LIU H CH,JIA W Q.Studies on POD,CAT of white clover stems under salt stress[J].Journal of Jilin Agricultural Sciences,2009,34(1):43-46.
[4] 王勇,李玉玲,郭平峰,等.NaCl盐胁迫对无核白葡萄生长的影响[J].中外葡萄与葡萄酒,2014(1):36-40. WANG Y,LI Y L,GUO P F,et al.Effect of NaCl stress on growth of Thompson seedless grape[J].Sino-Overseas Grapevine & Wine,2014(1):36-40.
[5] 张亚冰,刘崇怀,孙海生,等.葡萄砧木耐盐性与丙二醛和脯氨酸关系的研究[J].西北植物学报,2006,26(8):1709-1712. ZHANG Y B,LIU CH H,SUN H SH,,et al.Relation between salt tolerance of grape rootstock and MDA and proline contents in grape leaves[J].Acta Botanica Boreali-Occidentalia Sinica,2006,26(8):1709-1712.
[6] 孟昱,张钢,李雪平,等.NaCl胁迫对毛竹叶片的电阻抗图谱参数及膜透性的影响[J].武汉植物学研究,2010,28(3):341-346. MENG Y,ZHANG G,LI X P,et al.Effect of NaCl stress on electrical impedance spectroscopy parameters and membrane permeability of Moso bamboo(Phyllostachys edulis) seedling leaves[J].Journal of Wuhan Botanical Research,2010,28(3):341-346.
[7] 袁军伟,郭紫娟,刘长江,等.营养钵基部浸泡盐液法鉴定葡萄砧木的耐盐性及其生理响应[J].西南大学学报(自然科学版),2015,37(7):44-51. YUAN J W,GUO Z J,LIU CH J,et al.Evaluating the salt tolerance of grape rootstocks by base soaking of the plants with salt solution[J].Journal of Southwest University(Natural Science Edition),2015,37(7):44-51.
[8] ALIZADEH M,SINGH S K,PATEL V B,et al.In vitro responses of grape rootstocks to NaCl[J].Biologia Plantarum,2010,54(2):381-385.
[9] GONG H J,DEIDRE B,PETER C,et al.Contrast in chloride exclusion between two grapevine genotypes and its variation in their hybrid progeny[J].Journal of Experimental Botany,2011,62(3):989-999.
[10] WALKER R R,BLACKMORE D H,CLNGELEFFER P R,et al.Rootstock effects on salt tolerance of irrigated field-grown grapevines(Vitis vinifera L.cv.sultana).I.ion concentration in leaves and juice[J].Australian Journal of Agricultural Research,2002,8(1):3-14.
[11] UPRETI K K,MURTI G S R.Response of grape rootstocks to salinity:changes in root growth,polyamines and abscisic acid [J].Biologia Plantarum,2010,54(4):730-734.
[12] DOWNTON W J S,LOVEYS B R.Abscissic acid content and osmotic relations of salt-stressed grape vine leaves[J].Functional Plant Biology,1981,8(5):443-452.
[13] HATAMI E,ASHARI M E,JAVADI T.Effect of salinity on some gas exchange characteristics of grape(Vitis vinifera) cultivars[J].International Journal of Agriculture and Biology,2010,12(2):1560-8530.
[14] 周万海.葡萄砧木耐盐性及砧-穗特性的研究[D].兰州:甘肃农业大学,2005. ZHOU W H.Studies on salt tolerance of grape rootstock and characteristics of rootstock-scion interaction[D].Lanzhou:Gansu Agricultural University,2005.
[15] ALPER D,NURAY M,MüFTüOGLU,H,et al.Determination of salt tolerance of some American grape rootstocks[J].Bangladesh Journal of Botany,2006,35(2):143-150.
[16] 付晴晴,孙鲁龙,翟衡,等.葡萄种间杂交砧木育种F1代植株耐盐性分析[J].植物学报,2017,52(6):733-743. FU Q Q,SUN L L,ZHAI H,et al.Salt tolerant evaluation of F1-generation hybrids in grape[J].Chinese Bulletin of Botany,2017,52(6):733-743.
[17] 陈继峰.葡萄砧木品种的研究现状与展望[J].果树科学,2000,17(2):138-146. CHEN J F.The status of research on grape rootstock varieties and its prospect[J].Journal of Fruit Science,2000,17(2):138-146.
[18] 袁军伟,马爱红,郭紫娟,等.葡萄砧木抗逆性鉴定方法探讨[J].中外葡萄与葡萄酒,2011(11):66-75. YUAN J W,MA A H,GUO Z J,et al.Study on identification method of grape stock resistance[J].Sino-Overseas Grapevine and Wine,2011(11):66-75.
[19] 刘崇怀.葡萄种质资源描述规范和数据标准[M].北京:中国农业出版社,2006. LIU CH H.Description Standard and Data Standard of Grape Germplasm Resources[M].Beijing:China Agriculture Press,2006.
[20] 李会云,郭修武.盐胁迫对葡萄砧木叶片保护酶活性和丙二醛含量的影响[J].果树学报,2008,25(2):240-243. LI H Y,GUO X W.Influence of NaCl on activities of protective enzymes and MDA content in grape rootstock leaves[J].Journal of Fruit Science,2008,25(2):240-243.
[21] BELEW D,ASTATKIE T,MOKASHI M N,et al.Effects of salinity and mycorrhizal inoculation(Glomus fasciculatum) on growth responses of grape rootstocks(Vitis spp.)[J].South African Journal for Enology and Viticulture,2010,31(2):82-88.
[22] SOUTHEY J M,JOOSTE J H.Physiological response of Vitis vinifera L.cv.chenin blanc grafted onto different rootstocks on a relatively saline soil[J].South African Journal for Enology and Viticulture,1992,13(1):10-22.
[23] THOMAS W A.Development of a repeatable,low-cost,high-through put and precise salt tolerance assay for grapevines[D].Davis(USA):University of California:2011.
[24] 樊秀彩,刘崇怀,潘兴,等.水培条件下葡萄砧木对氯化钠的耐性鉴定[J].果树学报,2004,21(2):128-129. FAN X C,LIU CH H,PAN X,et al.Evaluation of salt tolerance of grape rootstocks under hydroponic culture conditions[J].Journal of Fruit Science,2004,21(2):128-129.
[25] 秦玲,齐艳玲,秦子禹,等.葡萄耐盐生理生化特性研究进展[J].河北科技师范学院学报,2011,25(3):75-80. QIN L,QI Y L,QIN Z Y,et al.Advances on physiological and biochemical characteristics of grape under salt stress[J].Journal of Hebei Normal University of Science and Technology,2011,25(3):75-80.