摘要
目的：小肠组织对射线极为敏感,核事故受照人员,白血病和腹腔局部放疗患者受到照射可引起对消化道特别是肠的损伤。电离辐射可导致小肠隐窝上皮细胞增殖抑制及部分细胞凋亡等异常改变,绒毛上皮的更新受阻,绒毛上皮结构的完整性遭到破坏,进而引起肠屏障功能障碍甚至引发菌血症和毒血症导致个体死亡。P38MAPK是重要的应激通路蛋白,在炎症中发挥着重要作用。本研究观察p38抑制剂SB203580对小鼠辐射致死效应和小肠损伤的保护作用。方法：小鼠辐射致死效应试验中C57BL/6小鼠按体重随机分为对照组,照射组和照射给药组,每组12只。对照组给予假照射,其它两组接受7.2Gy照射,照射前半小时腹腔注射SB203580(15mg/kg),以后隔天给药共5次。观察小鼠30天生存率。小肠损伤保护试验中小鼠随机分为对照组,照射组和照射给药组。对照组6只,其余各组8只。对照组为假照射,其它两组接受7.2Gy照射。给药组在照射前半小时腹腔注射SB203580(15mg/kg)。24小时后处死小鼠取小肠组织固定,脱水,并做石蜡切片。对切片进行HE染色,镜下计数每个隐窝内凋亡细胞数量,用免疫组化SP法检测caspase-3, Ki67, P53, p-p38表达,镜下计数隐窝阳性表达数。结果：对照组小鼠30天全部存活,照射组第11天全部死亡,照射给药组小鼠30天生存率为40.0%。照射组和照射给药组死亡小鼠平均生存天数分别为7.5d和13.6d。与照射组相比,照射给药组小肠隐窝细胞p-p38表达下降34.63%,p53表达下降21.78%,凋亡数量下降33.00%(caspase-3免疫组化染色)和30.14%(HE染色),Ki67表达升高37.96%,均有非常显著差异(p<0.01)
结论：结果表明SB203580特异抑制受照小鼠小肠隐窝细胞p38激活及p53表达升高,小肠隐窝细胞凋亡减少,增殖能力提高,30天小鼠生存率提高。首次证明p38抑制剂SB203580可有效保护辐射导致小肠辐射损伤。提示p38通路在辐射致死效应和辐射诱导小肠上皮细胞损伤中起着一定作用。p38抑制剂可能是潜在的辐射防护剂。
目的：血必净注射液具有拮抗内毒素、调节免疫功能、保护机体器官组织的作用。目前临床上广泛应用于感染及非感染因素所致全身炎症反应综合征,并取得了一定疗效。本研究观察血必净对辐射后ICR小鼠保护作用。方法：45只ICR小鼠按体重随机分为对照组,照射组,低剂量预防组组,低剂量治疗组和1/3低剂量预防组。对照组给予假照射,其它各组接受9Gy照射,预防给药组在照射前半小时给药一次,治疗给药组在照射后半小时给药一次,以后每天给药一次共10天。观察小鼠生存率。小肠损伤保护试验中小鼠随机分为对照组,照射组和血必净给药组,每组10只。对照组为假照射,其它两组接受9Gy照射。给药组在照射后半小时给药一次,给药剂量为0.4mg/kg。24小时后处死小鼠取小肠组织固定,脱水,并做石蜡切片。对切片进行HE染色,镜下计数每个隐窝内凋亡细胞数量。
结果：对照组小鼠30天全部存活。照射组和低剂量治疗组死亡小鼠平均生存天数分别为10.8 d和15.0 d。与照射组相比,照射给药组小肠隐窝细胞凋亡数量下降28.26%,均有显著差异(p<0.05)
结论：结果表明血必净可以提高小鼠受照后的生存率。给药后小鼠小肠隐窝细胞凋亡减少,这可能是提高小鼠照射后生存率原因之一。血必净有可能成为潜在的辐射防护剂。
To investigate the protective effects of the SB203580 against radiation induced mortality and intestinal injury. A total of 67 male C57/BL6 mice (20-22g) were matched according to body weight and randomly assigned to one of three groups: control, total body irradiation exposure (IR,7.2Gy) only, and IR+SB203580(15mg/kg). 30 days survival rate were observed in the experiment. In intestinal injury experiment, we assayed the caspase-3, Ki67, p53, p-p38 expression levels in the mice intestine crypts.We found that the 30 days survival rate were 100%(control),0 (IR) and 40% (IR+SB203580), respectively. Compared to the IR groups, the positive cells of caspase-3, P53 and p-p38 in crypt cells decreased 33.00%,21.78%and 34.63%, respectively. The positive cells of Ki67 increased 37.96%. Significant difference was found between all of them (p<0.01). SB203580 potently protected against radiation-induced lethal and intestinal injury in mice, and it may be a potential radioprotector.
To investigate the protective effects of the xuebijing injection against radiation induced mortality and intestinal injury. A total of 45 mice (20-22g) were matched according to body weight and randomly assigned to one offive groups: control, total body irradiation exposure (IR,7.2Gy) only, and IR (7.2Gy) +xuebijing(0.4ml/kg treatment group,0.4ml/kg Prophylaxis group,0.13ml/kg Prophylaxis group).30 days survival rate were observed in the experiment. In intestinal injury experiment, we assayed the opoptosis levels in the mice intestine crypts. We found that in low dose treatment group, the average survival time of mice died were 15days, and higher than the IR group (10.8 days), respectively. Compared to the IR groups, the apoptosis cells in crypt cells of low dose treatment group decreased 28.26%. Xuebijing injection potently protected against radiation-induced lethal and intestinal injury in mice, and it may be a potential radioprotector.
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