摘要
目的:探讨隐丹参酮单体对吗啡诱导的小鼠条件性位置偏爱效应的影响及成瘾过程中NO递质浓度改变与小鼠精神依赖的相关联系。
方法:利用条件性位置偏爱( conditioned place preference CPP )的方法,建立吗啡精神依赖模型。腹腔注射和侧脑室注射隐丹参酮单体,观察其对吗啡诱导的条件性位置偏爱效应形成和效应表达的影响。实验结束后用NO试剂盒测定小鼠全脑NO含量。
结果:(1)吗啡(6 mg/kg,sc)可诱导小鼠对伴药箱产生显著的位置偏爱,该模型是稳定可靠的。(2)无论是皮下注射还是侧脑室注射隐丹参酮单体均在一定程度上抑制小鼠CPP效应的形成和表达。(3)侧脑室注射隐丹参酮单体在一定程度上影响小鼠全脑的一氧化氮的水平,一氧化氮的水平与CPP效应呈一定关系。
结论(1)腹腔注射隐丹参酮可以抑制吗啡诱导的小鼠条件性位置偏爱效应的形成和表达,说明隐丹参酮有一定的抗吗啡依赖作用。(2)侧脑室注射隐丹参酮可以抑制吗啡诱导的小鼠条件性位置偏爱效应的形成和表达,说明隐丹参酮的抗吗啡依赖位置可能是在中枢起作用。(3)隐丹参酮抑制吗啡诱导的小鼠条件性位置偏爱效应形成和表达可能与改变小鼠大脑皮质NO递质水平有关。
subjective:To study the effect of Cryptotanshinone( icv and ip) on the acquisition and expression of morphine-induced conditioned place preference(CPP) and investigate the possible participation of nitric oxide in the acquisition of cpp in morphine-sensitized mice.
Methods: (1) Mice are administrated (sc) morphine (6mg.kg-1) to established a morphine-induced conditioned place preference (CPP) model. (2) administration(ip) Cryptotanshinone at different dose to investigate its effect on the acquisition and expression of cpp in morphine-sensitive mice.(3) administration(icv) Cryptotanshinone at different dose to investigate its effect on the acquisition and expression of cpp in morphine-sensitive mice. (40) after cpp acquisition experiment the encephalon of mice are processed with NO kit .
Results: (1)sc morphine 6mg/kg can establish a successful cpp model in morphine sensitive mice. (2) administration(ip) of Cryptotanshinone can dose-dependently inhibit the expression and acquisition of morphine-induced conditioned place preference in morphine-sensitive mice. (3) administration(icv) of Cryptotanshinone can dose-dependently inhibit the expression and acquisition of morphine-induced conditioned place preference in morphine-sensitive mice. (4) the level of nitric oxide change according to the administration(icv) of Cryptotanshinone at different dose during the acquisition of cpp in the morphine-sensitive mice.
Conclusion : (1)Cryptotanshinone can dose-dependently inhibited the acquisition and expression of morphine-induced cpp, the result show that the Cryptotanshinone can reverse the rewarding properties of morphine and the active position probably located at the central nerve systems.(2)It propose that the anti-morphine effect of Cryptotanshinone have some relationship with its effect on changing the level of nitric oxide.
引文
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