摘要
本文对豆科云实属植物喙荚云实的种子苦石莲Caesalpinia.minax Hance进行了较系统的化学成分研究,同时对豆科云实属植物的资源分布、化学成分、药理活性进行了综述。
本论文在前期药理活性筛选的指导下,应用现代色谱技术和方法对喙荚云实的种子苦石莲Caesalpinia.minax Hance提取物的活性部位进行了较系统的分离和纯化,共分离得到了28个化合物。通过现代波谱学方法技术(IR, UV, ESI-MS, HREI-MS, 1H-NMR,13C-NMR, APT, HSQC, HMBC, COSY, NOESY, TOCSY及X射线单晶衍射实验)鉴定了其中的21个化合物,其中包括9个卡山烷二萜：分别是Neocaesalpin K(K1), Neocaesalpin MO(K2), Neocaesalpin L(K3), Neocaesalpin A(K4), Neocaesalpin MP(K5), Neocaesalpin M(K6), Neocaesalpin MQ(K7), Spirocaesalmin(K8), Minaxin C(K9);6个黄酮类化合物：分别是槲皮素(K10), Minaxin(Kll), (+)-3,3',5',5,7-五羟基双氢黄酮(K12),5,7,4’-三羟基-黄烷-4-醇(K13),木犀草素(K14),3-甲氧基-5,7,3′,4′-四羟基-黄酮(K15)；同时,从中苦石莲分离得到了p-香树脂醇(K16),p-谷甾醇(K17),胡萝卜苷(K18),没食子酸(K19,),反式-9,12,13-三羟基-十八碳-10-烯酸(K20),3-(methoxycarbonyl)pent-3-enoic acid(K21);化合物K7,K9,K11为3个新化合物,化合物K21为首次从自然界分离得到,K12,K13,K14,K15,K20为首次从云实属植物中分离得到,化合物K10,K19为首次从苦石莲中分离得到。
此外,本文对苦石莲中卡山烷二萜类化合物的结构特征及波谱学规律进行了总结,对苦石莲化学成分的提取分离方法和生物活性研究进行了讨论。
Study on the Chemical Constituents of Caesalpinia.minax Hance
In this dissertation, the phytochemical studies were carried out on the medicinal plant of Caesalpinia.minax Hance. Meanwhile, the advance in the recent research of chemical constituents and pharmacological activities of Caesalpinia Linn, were reviewed.
In our research, the seed of Caesalpinia.minax Hance was studied systemically in chemistry by means of chromatography methods and spectral technologies. Twenty eight compounds were isolated from the MeOH extract. Twenty one compounds were determined by spectroscopic analysis and chemical methods, their structures were determined as follows:Neocaesalpin K(K1), Neocaesalpin MO(K2), Neocaesalpin L(K3), Neocaesalpin A(K4), Neocaesalpin MP(K5), Neocaesalpin M (K6), Neocaesalpin MQ(K7), Spirocaesalmin(K8), Minaxin C (K9), Quercetin (K10), Minaxin(Kll), (+)-3,3',5',5,7-pentahydroflavanone(K12),3,4-dihydro-2-(4-hydroxyphenyl)-2H-chromen e-4,5,7-triol (K13), Luteolin (K14),3-methoxy-5,7,3',4'-tetrahydroxy-flavone (K15),β-amyrin(K16),β-sitosterol (K17), Daucosterol (K18), Gallic acid (K19), 9,12,13-Trihydroxy-10(E)-octadecenoicacid(K20),3-(methoxycarbonyl)pent-3-enoic acid (K21); Compounds K7, K9, K11 were three new compounds, Compound K21 was a new natural product, Compounds K12, K13, K14, K15, K20 were obtained from Caesalpinia Linn, for the first time, Compounds K10, K19 were obtained from Caesalpinia.minax Hance for the first time.
In this paper, the structural characteristics and spectroscopic features and separation method for cassane from Caesalpinia.minax Hance were summarized.
引文
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