摘要
污泥中含有大量的有机物、重金属以及病原菌等,不加处理任意排放,会对环境造成严重的污染。近年来,我国城市污泥中的有害重金属含量已经明显下降,将污泥堆肥化处理后施用于园林绿地是较理想的土地利用方式,为了寻求污泥土地利用更广阔的途径,将污泥堆肥化处理后施用于蔬菜、作物是一个重要的研究内容。
本试验在借鉴前人利用污泥的经验基础上,通过田间微区实验探讨污泥堆肥对作物的生物效应和土壤环境效应,寻求污泥堆肥作为肥料的最佳施入量,对污泥堆肥施用后土壤、植物中的重金属总量及土壤中重金属形态的分布情况进行研究,进一步说明重金属在土壤植物系统中的分布情况,结果如下:
1.污泥堆肥施用后与化肥处理相比较油菜增产14.9%~28.3%,生菜增产5.5%~9.9%,小麦在污泥堆肥施用量为60t/hm2时增产12.54%,对于塿土来说,60t/hm2施用量增产效果比较明显,是较佳的施用量。
2.施用污泥堆肥后促进了油菜、生菜及小麦体内氮、磷含量的增加,增加了表层土壤中全氮、全磷、碱解氮、速效磷及有机质的含量。
3.污泥堆肥施用后引起了生菜、油菜、小麦植株中重金属在不同部位不同程度的累积,重金属进入植物体内主要分布在根系,其次才是茎叶,籽粒内含量往往最低,在利用污泥堆肥时,最好避开利用根部的植物。污泥堆肥施用未引起生菜和油菜叶内重金属超标,对于小麦,污泥堆肥施用量大于118t/hm2后超出国家卫生标准,叶中Cr,籽粒中Ni的含量和污泥堆肥的施用量间呈极显著正相关,在污泥堆肥的施用中存在的潜在隐患主要为Cr和Ni的累积污染。
4.施用污泥堆肥后,土壤中的Cu、Zn、Pb、Cd、Cr的含量显著提高,其中Cu、Zn、Cd和Cr和污泥堆肥施用量的相关关系达到显著水平,Ni的含量增加较少,这与污泥堆肥中的重金属含量紧密相关。小麦收获后重金属的迁移现象表明,土壤中Cu、Zn、Pb和Cd迁移较小仅至20cm,这说明重金属在土壤中的淋溶相当缓慢,污泥堆肥的施用在短期内不会有大量的淋溶。
5.污泥堆肥施用后重金属分配在不同的形态中,导致了各形态重金属含量的增加,主要增加了土壤中残留态重金属的含量。其余各形态中铁锰结合态居首,其次为有机结合态和碳酸盐结合态,可交换态的增加量最少,不同的重金属有所差异:Cd主要以铁锰氧化结合态和碳酸盐结合态存在,Zn和Pb以铁锰氧化结合态为主,碳酸盐结合态和有机结合态次之,Ni、Cu、Cr以铁锰氧化物结合态和有机结合态为主。
6.从潜在有效态(可交换态、碳酸盐结合态、铁锰氧化结合态、有机结合态)重金属占总量的比例来看,重金属的活性大小为Cd>Zn>Ni>Cu>Cr>Pb。从污泥堆肥施用后对土壤中重金属的活性影响看:Zn和Cr的活性提高分别为13%和1%,Pb和Cd的活性降低分别为9%和13%,Cu和Ni的活性基本无变化。
7.污泥堆肥施用后土壤中的重金属有效态含量与污泥堆肥施用量的关系呈显著正相关,有效态的含量:Zn>Cu>Pb>Ni>Cd,这与污泥堆肥和土壤中的重金属含量密切相关。
8.土壤重金属的可交换态、碳酸盐结合态、铁锰结合态、有机结合态、残留态和全量均能不同程度影响到土壤中重金属的有效性。各形态对有效态的作用大小,不同的重金属有所差异,Cu:有机结合态>铁锰结合态>残留态>可交换态;Zn:铁锰结合态>有机结合态;Pb:碳酸盐结合态>有机结合态;Cd:铁锰结合态>残留态>碳酸盐结合态;Ni:铁锰结合态>有机结合态>碳酸盐结合态,其余各形态对有效态的贡献相对较小。
There are lots organisms, heavy metal, pathogen and pathogenic bacterium in sludge, so it would pullout environment badly if it was discharged arbitrarily. In recent years, heavy metal content in sludge had decreased distinctly. It is reasonable and important of applying sludge compost on vegetable and crop for seeking wider approach of sludge using.
The study on the base of precursor's experience, through field experimental plot to discuss the biologic effect on crop and soil of sludge compost, seek the optimal application rate of sludge compost, research the content and form of heavy metal in soil and plant, furthermore explain the heavy metal distribution in soil-plant continuum. The results show:
1. Compared with inorganic fertilizer, the yield of rape and lettuce raised 14.9%~28.3% and 5.5%~9.9% respectively under application of sludge compost. Wheat yield increased 12.54% when the application rate was 60t/hm2, for Lou soil it was better.
2. The content of N and P in rape, lettuce and wheat, and total N, total P, available N, available P and organic matter in surface layer soil increased under application of sludge compost.
3. The application of sludge compost caused heavy metal accumulation in different part of rape, lettuce and wheat. Heavy metal content was more in plant root than stem and seed, so sludge compost should be applied on the plant that not using root. Heavy metal content in rape and lettuce were not over proofed, but when the application rate exceeded 118t/hm2, the content in wheat may exceed GB. It was remarkable correlation between sludge application and the content of Cr in leaf and Ni in seed. The accumulative pollution of Cr and Ni was the hidden trouble in sludge compost application.
4. The content of Cu, Zn, Pb, Cd, and Cr in soil applied sludge compost rose remarkably, and the correlation between sludge application and Cu, Zn, Cd and Cr was significance level. The addition of Ni was small, and it was closely correlative with the content of heavy
metal in sludge. Heavy metal eluviations after reaping was only 20cm, so we can say that it was little at short-term after sludge compost application.
5. Sludge compost application induced the increasing of content of various forms heavy metal; the rudimental form (R-) is the most. Amount the rest, the iron-manganese associative form (Fe-Mn) is the most, organic associative form (O-) is secondary and exchangeable form (Ex-) is the smallest. But various heavy metal were different, for example, iron-manganese oxidation associative form (Fe-Mn-Ox) and carbonate associative form (C-) is primary of Cd; Fe-Mn-Ox is primary and C- is secondary of Zn and Pb; Fe-Mn-Ox and O- is primary of Ni, Cu and Cr.
6. From the content rate of potential effective form (Fe-Mn-Ox, O-, C- and Ex-) with total heavy metal, heavy metal activity order was Cd>Zn>Ni>Cu>Cr>Pb. The activity of Zn and Cr rose 13% and 1% respectively, but Pb and Cd reduced 9% and 13% respectively, and Cu and Ni had no change under application sludge compost.
7. It was positive correlation between the application rate of sludge compost and the content of effective form heavy metal. The order of effective form content was Zn>Cu>Pb>Ni>Cd, and it was closely correlative with heavy metal content in sludge compost.
8. Fe-Mn-Ox, Om-, Cr- and Ex- heavy metal and total Zn affected heavy metal availability. Various metal and its form had different influence degree on availability. For example, the order of Cu is O-> Fe-Mn-Ox >R-> Ex-, Zn is Fe-Mn-Ox > O-, Pb is C->O-, Cd is Fe-Mn-Ox >R-> C- and Ni is Fe-Mn-Ox > O-> C-. The role of else form was indirect and smaller.
引文
[1]. 鲍士旦.土壤农化分析[M].中国农业出版社,北京,2002
[2]. 北京东南郊环境污染调查及防治途径研究协作组.北京东南郊环境污染调查防治途径研究(报告集)[C].1980,北京:中国科学院地理研究所
[3]. 曹仁林.园林绿地施用污泥堆肥对环境影响研究[J],环境科学研究. 1997,10(3):46~50
[4]. 曹秀芹,陈珺.污水处理厂污泥处理存在问题分析[J].北京建筑工程学院学报.2002,18(1):1~4
[5]. 陈华癸,樊庆笙.土壤微生物学[M].上海科技出版社.1981
[6]. 陈怀满,土壤-植物系统中的重金属污染[M]. 北京: 科学出版社, 1996: 300~307
[7]. 陈世俭,胡霭堂.长江流域资源与环境.1995.4NO.4
[8]. 陈同斌,黄启飞,高定,郑玉琪,吴吉夫.中国城市污泥的重金属含量及其变化趋势[J].环境科学学报,2003,23(5):561~569
[9]. 陈同斌,李艳霞,金燕,等.城市污泥复合肥的肥效及其对小麦重金属吸收的影响[J].生态学报,2002, 22(5): 643~648
[10].陈英旭,何曾耀,吴健平等.外源铬在土壤中的形态转化[J].农业环境保护,1995, 14 (3) : 102~110
[11].戈乃纷,曹洪生,吕春,等.污泥化肥混肥加工工艺和肥效的研究[J].土壤通报,1997, 28(1): 41~43
[12].戈乃玢,马淑芳,秦怀英,等.脱水污泥的组分和农用评价[J].农业环境保护,1995,14(5):202~206
[13].郭海洋.美国生活污水处理中污泥的利用(上).中国环境报,2002-06-28
[14].郭郿兰,田若涛,王雁卿,张青喜,赵逍红.城市污泥和污泥垃圾堆肥作为肥源,对作物重金属积累的影响[J].农业环境保护,1995,14(2):67~71
[15].国家环境保护总局,《处理厂污染物排放标准(报批稿)》,国家环境保护总局,2002年5月
[16].国家环境保护总局污染控制司编.城市固体废物管理与处理处置技术[M].北京:中国石化出版社,1999.262~272
[17].韩凤祥,胡霭堂,秦怀英,等.我国某些早地土壤中锌的形态及其有效性[J].土壤,1990,22(6):302~306
[18].何品晶,等.上海城市污水厂污泥处理与利用系统分析[J].长江流域资源与环境,2002,11(3):255~259
[19].姜城,杨金,陈振天,等.污泥、污泥复合肥农业应用的初步研究[J].吉林农业大学学报,1996,18(2):46~50
[20].蒋廷惠,胡霭堂,秦怀英.土壤锌铜铁锰形态区分方法的选择[J].环境科学学报,1990,10(3):280~286
[21].蒋廷惠.土壤中Cu、Zn、Fe、Mn的形态与有效性关系[J].土壤通报,1995, 5: 228~232
[22].金儒林.中国城市污水处理厂污泥处理的综述[J].武汉城市建设学院学报,1994,11(2):1~12
[23].金燕,李艳霞,陈同斌,等.污泥及其复合肥对蔬菜产量及重金属积累的影响[J].植物营养与肥料学报,2002,8(3):288~291
[24].孔维屏.土壤中Cu的形态及其转化[J].环境科学学报.1987,7(1):78-85
[25].冷平生,李树蓉.桧柏富集重金属研究初报[J].北京农学院学报,1995,10(1):87~92
[26].李承强,等.堆肥腐熟度的研究进展[J].环境科学进展,1999,7(6):1~12
[27].李贵宝,尹澄清,林永标,李志安.城市污泥对退化森林生态系统土壤的人工熟化研究[J].应用生态学报.2002,13(2):159~162
[28].李国学,黄焕忠,黄铭洪.施用污泥堆肥对土壤和青菜重金属积累特性的影响[J].中国农业大学学报,1998, 3(1 ):113~118
[29].李国学,孟凡乔,姜华,等.添加钝化剂对污泥堆肥处理中重金属(Cu、Zn、Mn)形态影响[J].中国农业大学学报,2000,5(1):105~111
[30].李艳霞,陈同斌,罗维,黄启飞,吴吉夫.中国城市污泥有机质及养分含量与土地利用[J]. 生态学报2003,23(11):2464~2475
[31].李艳霞,薛澄泽,陈同斌.污泥和垃圾堆肥用作林木育苗的研究[J].农村生态环境,2000,16(1):60~63
[32].李艳霞,赵莉,陈同斌.城市污泥堆肥用作草皮基质对草坪草生长的影响[J].生态学报,2002,22(6):797~801
[33].李艳霞.中国城市固体废弃物和资源化形势[J].农业工程学报,1998,14(增刊):279~284
[34].李应学,曹仁林,周毅,等.天津市农用污泥和土壤中有害物质安全控制标准的研究[J].农业环境保护,1988,7(1):12~18
[35].李宗利,等.污灌土壤中Pb、Cd形态的研究[J].农业环境保护,1994,(4) :152~157
[36].廖宗文,林东教,江东荣,等.广州市污泥垃圾农用资源化的初步研究[J].自然资源学报,1994,9(3):247~252
[37].林春野,董克虞,李萍,等.污泥农用对土壤及作物的影响[J].农业环境保护,1994,13(1):23~25,33
[38].林琦,等.根际坏境中镉的形态转化[J].土攘字报,1998,(4) :461~467
[39].刘风侠.林地处理城市污水污泥硝态氮在土壤中的迁移及对地下水影响[C].中国土壤学会第九次全国会员代表大会.天津市卷.1999
[40].刘善江,徐建铭,李亚星.污泥在大白菜上的应用的初探[J].北京农业科学,1994,12( 6):22~24
[41].刘善江.污泥在西红柿上的应用[J],北京农业科学,1996, 14(1): 43一44.
[42].鲁艳兵,温琰茂.施用污泥的土壤重金属元素有效性的影响因素[J].热带亚热带土壤科学,1998, 7(l): 68~71
[43].马娜,陈玲,熊飞.我国城市污泥的处置与利用[J]. 生态环境,2003,12(1):92~95
[44].欧盟官方网站2002年数据,http://europa.eu.int
[45].邱宏俊,郝以琼.国外污泥处置技术[J].重庆建筑大学学报,1998,20(6):51~55
[46].邵嫂庭,张进明,甘肃主要土壤中Cu、Zn、Mn、Fe形态及有效性研究[J].土壤学报,1995, 4: 423~429
[47].邵孝侯,邢光熹,侯文华.连续提取法区分土壤重金属元素形态的研究及其利用[J].土壤学进展,1994,22(3):40~46
[48].沈光范.城市污水处理厂的污泥处理和处置[J].中国环保产业2003,4:10~12
[49].数据引自《<城镇污水处理厂污染物排放标准(报批稿)>嘀扑得鳌?国家环境保护总局,2002年5月
[50].宋菲,等.镉、锌、铅复合污染对菠菜的影响[Jl.农业环境保护,1999,(1) :9~14
[51].苏德纯,张福锁,J.W.C.Wong.粉煤灰钝化污泥对土壤理化性质及玉米重金属累积的影响[J].中国环境科学1997.17(4):321~325
[52].谭启玲,胡承孝,周后建,陈黎,吴礼树.城市污泥中的重金属形态及其对潮土酶活性的影响[J].华中农业大学学报,2002,21,1:36~39
[53].田宁宁,王凯军,柯建明.剩余污泥好氧堆肥生产有机复混肥的肥分及效益分析[J].城市环境与城市生态,2001,14(1):9~11
[54].田宁宁,王凯军,杨丽萍,等.污水处理厂污泥处置及利用途径研究[J].环境保护,2000,2:18~20
[55].涂从.土壤砷毒性临界值的初步研究[J].农业环境保护,1992, 1(2):80~83
[56].王凯军,贾立敏.城市污水生物处理新技术开发与应用[M].北京:化学工业出版社,2001
[57].魏源送.堆肥技术及进展[J].环境科学进展,1999,7(3)
[58].吴吉夫,王淑坤,臧树良.城市污水处理厂污泥的有效利用和相关的环境问题研究[J].辽宁大学学报,2002,29(1):90~91
[59].肖玲,李岗,郝卫平.搂土施污泥对小麦生长的影响研究[J].西北农业大学学报,1994,22(1):89~93
[60].薛栋森.美国污水污泥的研究和利用概况[J].国外农业环境保护,19921,1
[61].薛文源.城市污水污泥处理与处置的途径[J].中国给水排水,1992,1(8):41~46
[62].严煦世.水和废水处理技术研究[M].北京:中国建筑工业出版社,1991.808~809
[63].杨惠芬,李名元,沈文食品卫生理化检验标准手册[M].北京:中国标准出版社,1997
[64].杨丽君.污水处理中污泥的处置与利用[J].绵阳经济技术高等专科学校学报,2001,18(3):26~29
[65].尹军,谭学军,廖国盘,翟佳麟,刘国栋.我国城市污水污泥的特性与处置现状[J].中国给水排水,2003,19,13:21~24
[66].张强.城市污泥施入土壤后营养元素的淋洗及对土壤肥力和地下水影响[C].中国土壤学会第九次代表会论文.山西土壤学会.1999
[67].张天红,薛澄泽,王秀琴,等.市政污泥用于城市绿化的可行性研究[J].环境杂志,1992,8(1):44~46
[68].张天红,薛澄泽.西安市污水污泥林用效果的研究[J].西北农业大学学报,1994,22(2):67~71
[69].张学洪,李金城,解庆林,等.城市污水污泥有机复合肥水稻施肥试验[J].广西科学,2000,7(3):232~234
[70].张增强,薛澄泽.几种草本植物对污泥堆肥的生长响应[J].西北农业大学学报,1996,24(1):65~69
[71].张增强,薛澄泽.几种木本植物对污泥堆肥的生长响应研究[J].西北农业大学学报,1995,23(6):47~51
[72].张增强,薛澄泽.污泥堆肥对几种草坪草生长的响应[J].草业学报,1997,6(1):57~65
[73].张增强等.污泥堆肥化处理对重金属形态的影响[J].农业环境保护,1996,15(4):188~190
[74].张自杰,林荣忱,金儒霖.排水工程(第3版)[M].北京:中国建筑工业出版社,1996
[75].赵莉,李艳霞,陈同斌,等.城市污泥堆肥在冷季型草皮专用复合肥生产中的应用[J].植物营养与肥料学报,2002,8(5):501~503,506
[76].赵丽君,张大群,陈宝柱.污泥处理与处置技术的进展[J].中国给水排水,2001,17(6):23~25
[77].赵书勤,吴星五.城市污水污泥堆肥化技术的研究进展[J].四川环境.2003,22,6(2):9~12
[78].周立祥,胡霭堂,戈乃玢,胡忠明.城市污泥土地利用研究[J].生态学报,1999,19(2):185~193
[79].周立祥,胡霭堂,戈乃玢.城市生活污泥农田利用对土壤肥力性状的影响[J].土壤通报,1994,25(3):126~129
[80].周立祥,张雪英,占新华,等.热喷处理污泥及其复混肥的养分效率与生物效应[J].环境科学学报,2001,21(1):95~100
[81].周立祥.中国城市污泥土地利用策略及其污泥重金属环境化学行为的机理研究.中国科学院地理研究所博士后研究工作报告.1998,北京
[82].朱燕婉.土壤中金属元素的五个组分的连续提取法[J].土壤1989.21(3)
[83].Bell P F. Heavy metal extractability in long term sewage sludge and metal salt-amended soils[J]. J Environ Qual, 1991, 20(2):481~486
[84].Bjarne P, Kjell T N W. Strategy for land application of sewage sludge in Norway[J]. Sci Tech, 1997, 36(11):283~290
[85].Canarutto S. How composting affects heavy metal content[J]. Biocycle, 1991, 32(6):48~50
[86].Chang A C. Sequential extraction of soils heavy metals following a sludge application[J]. J Environ Qual, 1984, 13:33~38
[87].Clapp. C. E.. S. A. Staek. D. E. Clay. andW. E. Larson. Sewage sludge organic matter and soil properties. P. Y. Chen and Y. Avnimelech(ed. )The role of organic matter in modern agriculture. Developments in Plant and soil sciences. Martinus Nijhoff Publ.. Dordrecht. the Netherlands. 1986, 209~253
[88].Consultants in Environmental Sciences. Limited UK Sewage Sludge Survey, Final Report[M]. London:Consultantsin Environmental Sciences Ltd. 1993
[89].Darmody R G. Municipal sewage sludge compost-amended soils: some spatiotem poral treatment effects[J]. J Environ Qual, 1983, 12(2):231~236
[90].Dar-Yuan Lee, Po-Hising Chiang, Kun-Huang Hong. Determination of bioavailable cadmium in paddy fields by chelating resin membrane embedded in soils[J]. Plant and Soil. 1996, 181:233~239
[91].Davis, R. D. The impact of EU and UK environmental pressures on future of sludge treatment and disposal[J]. Water Environ. Manage., 1996, 10(2)
[92].Dvidescu D. et al., 1982. Evalution of Fertility by Plant and Asis(Romania). Translated by Kent S. L., England, Abacus Press, 426~436
[93].Edward T, Nigel B Hetherington. Artifacts in the use of selective chemical extraction to determine distribution of metals between oxides of m anganese and iron[J]. Anal Chem, 1985, 57:1944~1946
[94].Emmorich W E. Solid phase of heavy metals in sewage sludge-treated soils[J]. J Environ Qual, 1982, 11:178~181
[95].EpsteinE., J. M. Taylor, andR. L. Chaney. Effects of sewage sludge and sludge compost applied to soil on some soil physical and chemical properties[J]. J. Environ. Qual. 1976, (54):422~426
[96].Fang M, Wong J W C. Effects of lime amendment on availability of heavy metals and maturation in sewage sludge composting[J]. Environ. Pollut. , 1999, 106(1):83~89
[97].Garcia, etal. Study on Water Extract of Sewage Sludge Composts[J]. Soil Sci. Plant Nutr., 1991, (37):399~408
[98].Hooka P S, Allowing B J. The Plant availablity and DTPA extractability of trace metal in sludge amended soils[J]. Sci Tot Environ, 1994, 149 (1-2):39~51
[99].Jason 2001, Jason M Conder, Roman P Lanno, Nicholas T Basta. Assessment of metal availability in smelter soil using earthworms and chemical extractions[J]. Journal of Environmental Quality. 2001, 30(4):1231~1237
[100].Katyal, J C and S C Agurwala, Micronutrient research in India. Fertilizer News. 1982, 27:66
[101].Klinc J R, R. H. Rust. Fraction of copper in neutron activated soils[J]. soils sci. Am proc. , 1966, 30:188~192
[102].Krishnamurti G S P, Huang P M, Van Rees K C J. Role of Fe-and AI-organic complexes in the availability of soil Cd. In: Proc. of 15th World Congress of soil Science Symp, Mexico: Intemational Society of Soil Science and Mexican Society of Soil Science, 1994, 3 (b):184~185
[103].Labrecque M. etal. Effect wastewater sludge on growth and heavy metal bioaccumulation of two soils species[J]. Plan tand Soil, 1995, 17(2):303~316
[104].Lee A W C. etal. Selected mechanical properties of wood produce by Loblolly pine trees fertilized with sludge[J]. Forest Products Journal, 1999, 49(9):43~47
[105].Mays, D. A., G. L Terman, andJ. C. Duggan. Municipal compost:effect on crop yield and soil properties[J]. J. Environ. Qual. 1973, 2(1):89~92
[106].Mcbride M B. Chemical sorption of Cd on oalcite surfaces. Soil Sci. Soc. Am. J, 1980, 44(1): 26~28
[107].Mclaren R G. D. V. Crawfold. Studies on soil copper: L The fraction of Cu in soil J. Soil Sci, 1973, 24:172~181
[108].Mininni G Santori M. Problems and perspectives of sludge utilization in agriculture[J]. Agric., Ecosystems and Environ, 1987, 18:291~311
[109].Mitch 2002, Mitch M Lasat. Phytoextraction of toxic metals: A review of biological mechanisms[J]. Journal of Environmental Quality. 2002, 31, (1):109~120
[110].N T Basta 1999, N T Basta, J J Sloan. Bioavailablility of heavy metals in strongly acidic soils treated with exceptional quality biosolids[J]. Journal of Environmental Quality. 1999, 28(2): 633~638
[111].Paliai M, G Guidi, M LaMarca, M Giachetti, and G Lucamantle. Effects of sewage sludge and composts on soil porosity and aggregation[J]. J. Environ. Qual. 1981, 10:556~561
[112].RAJ Arthur. Sludge disposal-where next[J]. W & WT, September, 1992, Vol, 35, No. 9
[113].Report from Member States to the Comm ission concerning sewage sludge treatment, November 1998, following directive 86/278/EEC on sewage sludge and ETC一W survey May, 2000
[114].Report from the Comm ission: Implementation of Council Directive 91/271/EEC of 21 May 1991 concerning urban waste water treatment, as amended by Commission Directive 98/15/EC of 27 February 1998. 15 January 1999. Inform ation from ET C/W survey May, 2000
[115].Report from the Commission: Implementation of Council Directive 91/271/EEC of 21 May 1991 concerning urban wastewater treatment, as amended by Commission Directive 98/15/EC of 27 February 1998. 15 January 1999. And for Sweden: Statistics Sweden Na 22 SM 9701, 2000
[116].Sally 2003, Sally Brown, Rufus L Chaney, Judith G Hallfrisch, Qi Xue. Effect of biosolids processing on lead bioavailability in an urban soil[J]. Journal of Environmental Quality. 2003, 32(1): 100 ~118
[117].Shuman L M, Zins manganeste and copper in noil fractions soil sci 1979. 127:10~17
[118].Sopper WE. 1989. Utilisation of sewage sludge in the United States for mine land reclamation. In:Hall JE. ed. Alternative Uses for Sewage Sludge. Pergamon Press. 21~40
[119].Tessier A W, etal. Sequential extraction procedure for the speciation of particulate trace metals[J]. Anal Chem, 1979, 51(7):844~851
[120].Tewier. Etal. Sequential extraction produce for the speciation of particulate trace metal[J]. Anal chem .. 1979. 51:844~850
[121].Vesilind P A, Hartman G C and Skene E T. Sludge Management and Disposal for the Practicing EngineerJM]. Michigan (USA): Lewis Publisher. 1986
[122].Vigerust E. Physical properties in sludge and sludge treated soils. In The Influence of Sewage Sludge Applicationon Physical and Biological Properties of Soils. D. Reidel
Publishing Company. 1983
[123].Webber M D, Nichols J A. Organic and metal contaminants in Canadian municipal sludges and sludge compost[M]. Burlington:Wastewater Technology Centre. 1995