摘要
针灸是中华民族的瑰宝,它具有独特的疗效和丰富的内涵,是我国具有特色和优势的学科之一。针灸医学已进入现代科学的领域,引起了各国医学家极大的关注和兴趣。但是,当前针灸疗效机理研究多为统计学意义上的比较研究,缺乏规律性、系统性的归纳和量化。目前虽然脑电图仪、心电图仪、肌电图仪等均能在一定程度上反映针刺的作用和疗效,但其采样频率都在200Hz以下,还不能满足利用多点、同步电极研究体表电信号针刺传导与作用规律的要求。
本文首先从应用屏蔽线将体表电信号引入初级放大器开始,提出一种多点同步体表电信号检测装置,然后对各级放大器的放大倍数及电阻,电容的正确配比和噪声的大小进行详细的理论分析、仿真和实验;利用采样定理计算出合理的采样频率,并提出运用复杂的可编程逻辑器件(CPLD)控制模拟数字转换器(AD),将模拟信号转化成数字信号。在此基础上,本文首先通过CPLD对先入先出存储器(FIFO)进行控制,实现数据缓冲;然后利用高速的DSP将数据转存到大容量的同步动态随即存数器(SDRAM)中,最后,通过通用串行总线(USB)接口芯片将数据上传至PC。
本文基本上实现了一种高速,多路,同步采集的体表电信号检测装置,所研究的装置具有高精度、高抗干扰的特点,为量化不同针刺手法及疗效,揭示针刺电信息传导与作用规律提供了重要的基础。
Acupuncture is a Chinese treasure which has a particular good curative effect and plenty of connotations. It has now entered the field of modern science and attracts much attention from foreign medical scientists. However, current research mostly focuses on the comparison with the statistics method which lacks of the regular, systematic conclusion and quantification. Although currently, the EEG, ECG, EMG and so on can all respond to the acupuncture's effect at a certain extent, but their sample rates are all less than 200Hz and they aren’t fit for detecting and processing the multi-spot and synchronized signal.
The thesis begins with introducing the electrical signal to the first equipment amplifier and develops an equipment which can detect and process the multi-spot and synchronized signal at a high speed. The thesis calculates the amplifier gain, resistance value, capacitance value and analyzes the noise value with detailed academic analysis. Then it simulates and realizes the whole analog system. By careful analysis, it gets the calculated sampling rate. Subsequently the analog data is converted to digital data with AD controlled by CPLD. After getting the digital data, these data are cushioned by FIFO and are stored into SDRAM by DSP. At last, these data is passed to PC by USB port.
The design realizes a multi-spots synchronization high-speed collecting system which is high-precision and anti-jamming. The design is the requirement of quantify various acupuncture manipulations. It contributes to reveal the conduction and effect ion of the surface electrical signal.
引文
[1] Nicholas J. Kaplinsky and M. Kathryn Barton, Plant Acupuncture: Sticking PINs in the Right Places, Science, Vol.306, No.29, October, 2004, pp.822-823
[2] Knardahl Stein, Elam Mikael, Olausson Bengt et al, Gunnar, Sympathetic nerve activity after acupuncture in humans, Pain, Volume.75, Issue: 1, March, 1998, pp.19-25
[3] Mori Hidetoshi, Nishijo Kazushi, Kawamura Hiroki et al, Unique immunomodulation by electro acupuncture in humans possibly via stimulation of the autonomic nervous system, Neuroscience Letters, Volume: 320, Issue: 1-2, March 1, 2002, pp.21-24
[4] Ernst Edzard, and White Adrian R., Prospective studies of the safety of acupuncture: a systematic review, The American Journal of Medicine, Volume: 110, Issue: 6, April 15, 2001, pp.481-485
[5] Toda, Kazuo and Afferent, Nerve characteristics during acupuncture stimulation, International Congress Series, Volume: 1238, August, 2002, pp.49-61
[6]王世惠,中医针灸学,上海:上海科技教育出版社,1995
[7] Ji-Sheng Han, Acupuncture: neuropeptide release produced by electrical stimulation of different frequencies, TRENDS in Neurosciences, Vol.26, No.1, January, 2003
[8] Wan You, Wilson Sonya G., Han Ji-Sheng, et al, The effect of genotype on sensitiveity to electroacupuncture analgesia, Pain, Volume: 91, Issue: 1-2, March, 2001, pp.5-13
[9] Huang Cheng, Wang Yun, Han-Ji Shen, Characteristics of electro acupuncture induced analgesia in mice: variation with strain, frequency, intensity and opioid involvement, Brain Research, Volume:945, Issue: 1, July 26, 2002, pp.20-25
[10] Wang Jin-Yan, Luo Fei, Chang Jing-Yu, et al, Parallel pain processing in freely moving rats revealed by distributed neuron recording, Brain Research,Volume: 992, Issue: 2, December 5, 2003, pp.263-271
[11] Zhang Wei-Ting, Jin Zhen, Cui Guo-Hong, et al, Relations between brain network activation and analgesic effect induced by low vs. high frequencyelectrical acupoint stimulation in different subjects: a functional magnetic resonance imaging study, Brain Research, Volume: 982, Issue: 2, August 29, 2003, pp. 168-178
[12]石学敏,傅立新,赵铭辉等,“醒脑开窍”针法对实验性脑缺血大鼠心肌超微结构损伤的保护作用,中国针灸,Vol.18, No.7, 1998, pp.405-408
[13]石学敏,韩景献,李平等,针刺对老化痴呆鼠脑兴奋性氨基酸水平影响的实验研究,中国针灸. Vol.18, No.11, 1998, pp.689-692
[14]石学敏,针灸临床研究进展概述,针刺研究. Vol.23, No.2, 1998, pp.85-90
[15]石学敏,新世纪针灸学科面临的问题和对策,中医药学刊,Vol.20, no.1, 2002, pp.7-8
[16]李忠仁,针灸学术发展的动力是科学实验,南京中医药大学学报. Vol.20, No.1, 2004, pp.1-4
[17]李忠仁,彭拥军,李忠仁教授学术思想探讨,针灸临床杂志. Vol.19, No.12, 2003, pp.3-4
[18]叶小卫,针刺体表电信号的数字检测系统的设计,天津大学硕士学位论文. 2006
[19]大熊辉雄,脑电图判读Step by Step入门篇(原书第三版)周锦华译,北京:科学出版社,2001
[20]张开滋,郭济鸿,刘海洋等,临床心电学,长沙:湖南科学技术出版社,2002
[21]牛永红,李章勇,谢正祥,QTD自动测量与人工测量对比研究,中国医学物理杂志,19(3),pp.172-173
[22]徐晓红,谢正祥,陈良迟等,心动周期信号的混沌特征分析及应用,北京:科学技术文献出版社,1992
[23]徐晓红,谢正祥,陈良迟等,正常人心动周期的混沌特征随年龄变化,生物医学杂志,1996,13(1):25
[24]徐晓红,谢正祥,陈良迟等,心动周期信号的混沌特征分析及应用,中国生物医学工程学报,1999,18(1):74
[25]谢正祥,陈良迟,张能等,三维胃电信号的动态频谱及平均周期图分析,中国医学物理学杂志,1993
[26]谢正祥,计算机在胃肠电信号处理中的应用,广州:广东科技出版社,1998
[27]周吕,胃肠生理学,北京:北京出版社,1991
[28] Charles Kitchin,Lew Counts,A Designer’s Guide To Instrumentation Amplifiers(2nd),Analog Devises
[29]任艳颖,王彬,IC设计基础,西安:西安电子科技大学出版社,2003
[30] Donald E. Thomas,Philip R. Moorby著,刘明业,蒋敬旗,刁岚松等译,硬件描述语言Verilog,北京:清华大学出版社,2001
[31] Michael D. Ciletti Ciletti, Michael D.,Advanced digital design with the Verilog HDL, Beijing: Publishing House of Electronics Industry, 2004
[32]陈启美,丁传锁,计算机USB接口技术,南京:南京大学出版社,2003
[33]肖踞雄,翁铁成,宋中庆,USB技术及应用设计,北京:清华大学出版社,2003
[34]张念淮,江浩,USB总线接口开发指南,北京:国防工业出版社,2001
[35] Analog Device, Single Supply, Rail-to-Rail, Low Cost Instrumentation Amplifier AD623, 1999
[36] National Semiconductor Corporation, An Applications Guide for OP Amps, 2004
[37] Burr-Brown Products from Texas Instruments, 0.05μV/.C max Signal-Supply CMOS Operational Amplifiers Zero-Drift Series, 2003
[38]远坂俊昭,测量电子电路设计模拟篇从OP放大器实践电路到微弱信号的处理,北京:科学出版社,2006
[39] Texas Instruments, Real World Signal Processing医疗应用之产品信息,2004, pp17
[40] Emmanuel C. Ifeachor, Barrie W. Jervis,Digital signal processing : a practical approach,北京:电子工业出版社2004
[41] Burr-Brown Products from Texas Instruments, 16-Bit 4-Channel Serial Output Sampling Analog-To-Digital Converter, 2002
[42]刘明业,集成电路/计算机硬件描述语言VHDL高等教程,北京:清华大学出版社,2003
[43]赵俊超,集成电路设计VHDL教程,北京:北京希望电子出版社,2002
[44]郑亚民,董晓舟,可编程逻辑器件开发软件Quartus II,北京:国防工业出版社,2006
[45]王开军,姜宇柏,面向CPLD/FPGA的VHDL设计,北京:机械工业出版社,2007
[46] Integrated Device Technology, 3.3 Volt CMOS SyncFIFOTM, 2002
[47] Hynix, 4bank×1M×16bits Synchronous DRANM, 2005
[48] CYPRESS, EZ-USB SX2TM High-Speed USB Interface Device CY7C68001, 2004
[49] Chris Cant著孙义,马莉波,国雪飞等译,Windows WDM设备驱动程序开发指南,北京:机械工业出版社,2000
[50]武安河,WINDOWS2000/XP WDM设备驱动程序开发(第2版),北京:电子工业出版社,2005