摘要
目的:探讨中药提取物葛根素注射液对大鼠视网膜光损伤的保护作用及其机理。
方法:通过光镜观察各组间视网膜外核层组织形态学的改变和免疫组化检测细胞凋亡调节因子Bax、Bcl-2、Bcl-2/Bax、Caspase-3的表达。
结果:①组织形态学上,模型对照组较正常对照组视网膜外核层变薄,细胞排列稀疏,出现较多染色不规则或破碎的细胞核,内外节排列紊乱,分界不清,两者差异有统计学意义(P＜0.05);低剂量治疗组视网膜形态较模型对照组有改善,外核层增厚,细胞排列紧密,两者差异有统计学意义(P＜0.05);高剂量治疗组视网膜形态较模型对照组无明显改善,外核层薄,细胞排列稀疏,内外节排列紊乱,分界不清,两者差异无统计学意义(P＞0.05)。②调控细胞凋亡因子Bax、Bcl-2、Caspase-3的表达,光损伤后,模型对照组视网膜外核层胞浆中Bax、Caspase-3表达增强,Bcl-2表达及Bcl-2/Bax的比值减弱,与正常对照组组间差异有统计学意义(P＜0.05)。低剂量治疗组视网膜外核层胞浆中Bax、Caspase-3表达有所减少,Bcl-2表达及Bcl-2/Bax的比值增强,与模型对照组组间差异有统计学意义(P＜0.05)。高剂量治疗组视网膜外核层胞浆中Bax、Caspase-3表达增强,Bcl-2表达及Bcl-2/Bax的比值减弱,与模型对照组组间差异无统计学意义(P＞0.05)。
结论:本实验结果提示葛根素注射液具有减轻视网膜光损伤,以及促进光损伤后视网膜修复的作用。其作用机理是通过影响Bax、Bcl-2、Caspase-3表达及Bcl-2/Bax的比值,抑制感光细胞凋亡有关。
Objective.To approach the protection and its mechanism of Puerarin against retinal damage by light.
Methods:Observed the histomorphplogy changes of outer nuclear layer and detected the regulatory factors of apoptosis Bax,Bcl-2,Bcl-2/Bax and Caspase-3.
Result:①In histomorphplogy,the model group's became thinner compared normal group's and cell arrangement was raritas,internal and external segments arrangement was disordered,and the demarcation wasn't distinct,the differences had statistical significance compared normal group's(P＜0.05).Low-dose group's had improvation compared model group's,the outer nuclear layer of retina become thicker,the cell are closely,the differences had statistical significance compared model group's(P＜0.05).High-dose group's had no obvious improvement compared model group the differences had no statistical significance compared model group's(P＞0.05)②In expression of regulating apoptosis of Bax、Bcl-2、Caspase-3 factors,after light postinjury,the expression of Bax,Caspase-3 in outer nuclear layer kytoplasm of model group's reinforced, expression of Bcl-2 and the ratio of Bcl-2/Bax weakened,the differences had statistical significance compared normal group's(P＜0.05).Low-dose group's decreased,expression of Bcl-2 and the ratio of Bcl-2/Bax reinforced,the difference had statistical significance compared model group's(P＜0.05). High-dose group's Bax,Caspase-3 reinforced,Bcl-2 and the ratio of Bcl-2/Bax weakened,the differences had no statistical significance compared model group's(P＞0.05)
Conclusions:Low-dose Pue could release the photic injury in retina,accelerate the renovation of retina after light exposure.The mechanism might relate to control the expression of Bax,Bcl-2,Bcl-2/Bax and Caspase-3 and suppresse photosensory cell apoptosis.
引文
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