摘要
针灸疗法是中医学体系的一个重要组成部分,对一些疾病(例如慢性疼痛)具有良好的治疗效果。针灸效应作用机制至今尚未解决。研究针灸效应的细胞响应有助于理解针灸效应机理,推动针灸经络相关研究,促进传统中医学的发展。
本文采用光学检测技术研究针灸效应在细胞水平的生理表征。首先,利用激光扫描共聚焦显微成像技术检测了活细胞中针灸效应作用分子一氧化氮的含量,并对比了不同类型细胞中一氧化氮含量的差异。其次,研究了一氧化氮对Hela细胞内钙离子浓度的调节作用。研究表明一氧化氮能诱导细胞内钙离子浓度升高,而且一氧化氮分子诱导钙离子浓度升高是细胞外钙离子内流和细胞内钙库释放钙离子共同作用的结果。内部钙库释放钙离子是钙离子浓度升高的主要影响因素。研究还表明线粒体是一氧化氮分子引起细胞内部释放钙离子的一种钙库。再次,基于生物发光共振能量转移技术研究了腺苷在激光针灸效应中的作用。研究发现激光辐照能抑制腺苷含量,且抑制程度与辐照光剂量呈正比。此外,腺苷A3受体拮抗剂能增强激光辐照对腺苷的抑制作用。最后,利用数学模型模拟了激光辐照对动作电位的影响。结果表明激光辐照能缩小动作电位的周期,激光辐照剂量对动作电位传输特性有一定的影响。较高辐照光剂量能够抑制动作电位的传输,而较小辐照光剂量能促进动作电位的传输。
本文探索了针灸效应的若干细胞响应,研究结果有助于在细胞分子水平认识针灸效应的相关机理。
Acupuncture is an important complementary and alternative medicine, which has good therapy results for some kinds of diseases, such as chronic pain. The mechanisms of acupuncture are still unclear. Studying the mechanisms of acupuncture could be helpful to understand the role of acupuncture and to push forward the research of acupuncture meridian and to improve the development of traditional Chinese medicine.
Optical techniques had been utilized to study the cellular response to acupuncture in this work. Firstly, the nitric oxide (NO, which plays a crucial role in many acupuncture therapies) in live cells was measured by laser scanning confocal microscopy. The concentrations of NO in different kinds of cells were compared. Secondly, the modulation of NO on the calcium in Hela cells was investigated. The results showed that NO could increase the concentration of calcium by calcium influx and calcium release from intracellular calcium storages. Calcium releasing from intracellular calcium storages is dominant. And mitochondrion is one of the calcium storages that take part in the modulation of NO. Thirdly, the role of adenosine in laser acupuncture was revealed by measuring the dynamics of cAMP by bioluminescence resonance energy transfer. The results indicated that laser irradiation could inhibit the level of cAMP in light dose dependent manner. Moreover, A3receptor antagonist could enhance the inhibition of laser irradiation. Finally, simulation of the effect of laser irradiation on action potential characteristics was performed. The simulation results showed that laser irradiation could decrease the period of action potential. The effect of laser irradiation on action potential transmission is depended on the light dose, which could play inhibition or stimulation role.
This work revealed preliminarily the cellular response of acupuncture effects. It is undoubtedly helpful to understand the mechanism of acupuncture on the cellular and molecular level.
引文
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