摘要
目的:
<1>观察卡托普利对心律失常的防治作用
<2>观察卡托普利和胺碘酮联合用药对心律失常的防治作用
方法:
采用氯化钡或乌头碱诱发大鼠心律失常,以发生室性心律失常的持续时间作为指标,观察不同剂量卡托普利对胺碘酮抗心律失常作用的影响。
⑴对氯化钡模型的影响:大鼠80只,随机平均分为8组:①生理盐水组;②3.125mg·kg-1卡托普利组;③6.25mg·kg-1卡托普利组;④12.5mg·kg-1卡托普利组;⑤胺碘酮组;⑥3.125mg·kg-1卡托普利+胺碘酮组;⑦6.25mg·kg-1卡托普利+胺碘酮组;⑧12.5mg·kg-1卡托普利+胺碘酮组。麻醉动物,静注氯化钡。分别静注不同组的药物。用RM-6240-C型多道生理信号采集处理系统连续观察Ⅱ导ECG,记录并分析室性心律失常的持续时间。
⑵对乌头碱模型的影响:大鼠80只,随机平均分为8组:①生理盐水组;②3.125mg·kg-1卡托普利组;③6.25mg·kg-1卡托普利组;④12.5mg·kg-1卡托普利组;⑤胺碘酮组;⑥3.125mg·kg-1卡托普利+胺碘酮组;⑦6.25mg·kg-1卡托普利+胺碘酮组;⑧12.5mg·kg-1卡托普利+胺碘酮组。麻醉动物,静注乌头碱。分别静注不同组的药物。用RM-6240-C型多道生理信号采集处理系统连续观察Ⅱ导ECG,记录并分析室性心律失常的持续时间。在乌头碱模型,预先静注胺碘酮基础上静注卡托普利同样可以缩短心律失常持续时间,增大卡托普利剂量,心律失常的持续时间进一步缩短(差异均有统计学意义P < 0. 05)。
结论:使用卡托普利可以防治室性心律失常。卡托普利使胺碘酮对抗氯化钡诱导的大鼠室性心律失常的作用明显增强。应用卡托普利可增强胺碘酮抗心律失常的作用,此增强作用对卡托普利具有明显的剂量依赖性,加大卡托普利剂量,药物疗效可进一步增强。
Objective: To study the influence of antiarrhythmic effects of intragastric administration Captopril to Amiodarone on ventricular arrhythmias and mechanism of the effect. To provide experimental evidence for different of administration on clinic.
Methods: adopt arrhythmia model of BaCl2 or Aconitine and index of ventricular arrhythmias emergence time and category, apply Amiodaroneon by vein after withdrawal Captopril. To observe the influence of antiarrhythmic effects of different dose of Captopril to Amiodarone on two arrhythmic model.
(1) To BaCl2 arrhythmia model:80 rat, to divide equally and probabilisticly:①normal sodium group;②3.125mg/kg Captopril group;③6.25mg/kg Captopril group;④12.5mg/kg Captopril group;⑤Amiodarone group;⑥3.125mg/kg Captopril +Ami group;⑦6.25mg/kg Captopril +Ami group;⑧12.5mg/kg Captopril +Ami group. Withdrawal them , iv Amiodarone then iv BaCl2 five minte late . observe theⅡl ead ECG continuously by RM-6240-C-type multichannel system of physiology information, memorize and analyze emergence time and category of arrhythmias.
(2) To aconitine arrhythmia model:80 rat, to divide equally and probabilisticly:①normal sodium group;②3.125mg/kg Captopril group;③6.25mg/kg Captopril group;④12.5mg/kg Captopril group;⑤Amiodarone group;⑥3.125mg/kg Captopril +Ami group;⑦6.25mg/kg Captopril +Ami group;⑧12.5mg/kg Captopril +Ami group. Withdrawal them , iv Amiodarone then iv aconitine five minte late. observe theⅡl ead ECG continuously by RM-6240-C-type multichannel system of physiology information, memorize and analyze emergence time of arrhythmias.
Results: (1)To BaCl2 arrhythmia model: with the application and dose increasing of Captopril, Amiodarone prominently as lighten ventricular tachyarrhythmias to ventricular premature pulse(P < 0. 05).
(2)To aconitine arrhythmia model: intragastric administration Captopril prominently reduced the duration;with the dose increasing of Captopril, Amiodarone reduced the duration further(P < 0. 05).
Conclusion: To BaCl2 and Aconitine arrhythmia model, the effect of Amiodarone on antiarrhythmias were markedly contributed by Captopril, the contributed effect have a quality of dose dependent when dose of Captopril increase markedly. With the dose increasing of Captopril, therapeutic effect may enhancement further, but enhance possibility of bring about negative inotropic frequency conduction effect greatly.
引文
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