摘要
重视天然药物的研究是国际新药研究的重要趋势,据统计,目前临床应用的药物中,有一半左右是来自于天然产物或其衍生物。本实验对薯蓣皂苷的抗肿瘤作用进行了系统研究。体外活性试验结果表明,薯蓣皂苷对Lewis肺腺癌细胞的生长具有抑制作用,并显示一定的细胞毒选择性和结构-细胞毒作用关系。IC_(50)为3.85±0.11μg/mL。此外,其对Lewis肺腺癌细胞的肿瘤细胞迁移能力也存在剂量依赖性作用。结果表明在0.2～1.0μg/mL的浓度范围内,药物可减缓癌细胞的迁移趋势或速度。
采用Lewis小鼠肺腺癌模型对甾体皂苷类化合物进行的体内抗肿瘤活性试验中,薯蓣皂苷能显著抑制实体瘤的生长(60mg/kg/d连续给药14天,抑瘤率为30.68%),抑制实体瘤细胞的生长及转移,未见明显毒性。总之,薯蓣皂苷在抑制肿瘤细胞向肺组织的转移方面显示了其优势。
With the previous researches,dioscin has been found to show strong immunoloregulative activity.However,dioscin whether or not has some antitumor effects is not known.To detail the effective mechanisms of dioscin,we investigated the in vitro experiment including MTT assay and wound healing assay.In our experiments, Lewis cells were exposed to dioscin.Cytotoxicity was determined in terms of cell growth inhibition(IC_(50)).The results of MTT assay showed that dioscin could inhibit proliferation of Lewis cancer cell line in different degree and it had dose-cytotoxicity relationship in a certain degree.The IC_(50) was 3.85±0.11μg/mL.Migration inhibition was evaluated by wound healing assay in non-cytotoxic dose.The results demonstrated that dioscin exhibited remarkable cytotoxicity and caused typical apoptosis in a dose-dependent manner,whose concentration ranging from 0.2 to 1.0μg/mL.
Dioscin was evaluated in vivo by its antitumor effect on Lewis inbred mice.In the tumor weight,spleen index,antitumor rate and numbers of mets comparison,the oral administration to mice of dioscin(60 mg/kg/d for 14 days) significantly inhibited tumor growth by 39.44%.The spleen index was 6.23mg/kg which was similar to the model group.It also could inhibit Lewis cell metastasis to lung and have no toxicity on main organs.In conclusion,dioscin exhibited strong antitumor and anti-metastasis in lung cancer mice.
The study focused on the investigation of the antitumor effects of dioscin.The results of MTT assay showed that dioscin could inhibit proliferation of Lewis cell line in different concentration and it had dose-cytotoxicity relationship in a certain degree.The IC_(50) was 3.85±0.11μg/mL.In addition,it exhibited anti-migration in the wound healing assay in different concentration ranging from 0.2 to 1.0μg/mL
Dioscin was evaluated in vivo by its antitumor effect on Lewis mice.The oral administration to mice of dioscin(60 mg/kg/d for 14 days) significantly inhibited tumor growth by 30.68%.It also could inhibit Lewis cell transfer to lung and has no toxicity on main organs.
In conclusion,dioscin is a potent anticancer agent that inhibits the migration in murine lung adenocarcinoma,both in vitro and in vivo.Dioscin exhibited strong antitumor and anti-metastasis in lung cancer mice.
引文
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