苹果品质的超微弱光无损检测技术研究
详细信息    本馆镜像全文|  推荐本文 |  |   获取CNKI官网全文
摘要
随着果品产业的飞速发展,果品品质越来越受到果农、果商和消费者的重视,也为品质检测提出了更高要求。本文通过研究苹果微弱光特性的变化,探讨利用微弱光无损检测技术来判断苹果内部品质的方法。
     本文以富士苹果为对象,利用单光子计数探测系统,研究了测试条件、预处理方法、等因素对苹果超微弱光特性参数的影响;通过大量试验,初步探究了苹果在储藏阶段的超微弱光值变化与内部品质的关系;研究了不同损伤条件下苹果微弱光特性的变化。得到以下研究结论:
     1.苹果超微弱发光(UL)与照射光源的种类、照射强度、照射时间、距离等测试条件有密切关系。果实的超微弱发光值随时间延长而衰减,用双指数曲线可以较好地拟合苹果的微弱光随时间的衰减关系;光强越强,时间越长,UL值越大;白炽灯照射比荧光灯照射苹果的UL值大,光源照射距离对UL值的影响不显著。
     2.苹果品种、生长位置、成熟度、测试部位、温度等因素影响苹果超微弱发光特性。暗室贮藏比在正常环境下UL值要高;温度越低,UL越小;早熟品种的UL高,晚熟品种较低;赤道与果蒂处的UL值低,果梗处的UL值高;树冠顶部苹果的UL值最大,中部次之,底部最弱,树冠南面和西面的UL值大,东面次之,北面最弱;随着果实成熟度增加,其UL值下降。
     3.新鲜苹果的糖度、酸度、硬度、可溶性固形物含量、pH值等品质指标与超微弱发光的关系不显著。
     4.苹果贮藏期的超微弱光变化显著。果实在采后成熟过程中超微弱发光有明显的峰值出现;随着贮藏时间延长,UL值不断减少;随着苹果硬度下降、pH值下降、可溶性固形物缓慢增加,其UL值下降,利用微弱光值的大小可以判断苹果新鲜度等级。
     5.破损对称苹果超微弱光值有明显影响。损伤瞬间,苹果的超微弱光值急剧变化;撞击损伤果的微弱光值随时间变化明显,静压损伤果随时间变化不显著;损伤面积较大以及损伤深度较大的果品,UL值变化明显;储藏期间,损伤苹果的UL值持续降低,无损苹果的UL则变化的非常缓慢,利用微弱光值的大小可以判断苹果损伤。
With the rapid development of the fruit-industry, the quality of fruits has been attached more and more importance to fruit farmers, fruiterers and consumers. And this asks for the higher standard for the examination of fruits. In this article, we use the nondestructive determination technology to judge the characteristics of apples by studying the change of the ultra weak bioluminescence(UL) parameters of apples.
     This paper aimed to Fuji apple, using single photon counting testing system, studying infection of the ultra weak bioluminescence(UL) parameters of apples, such as test conditions, pretreatment methods, types of fruit etc; We also studying the relationship between the ultra weak bioluminescence(UL) and the physical character during the storing by lots of experiments; We studying changes in the the ultra weak bioluminescence(UL) of Apples by different injury condition. The result shows:
     1. Apple’s ultra weak bioluminescence(UL) and the type of source, the radiation intensity, exposure time, distance test conditions closely related. The ultra weak bioluminescence of apples decay with time, and with double exponential curve fitting can be used to Apple's UL attenuation relationship with the passage of time. The stronger light intensity, the longer the time, the greater the UL value; incandescent light have more affection than fluorescent radiation light on apple’s UL value. The distance of light irradiation impact on the UL is not significant;
     2. Apple varieties, growth position, maturity, test location, temperature and other factors affect Apple's ultra weak bioluminescence(UL) properties.UL of apple stored in the darkroom is higher than in the normal environment; the less temperature, the lower UL of apples; Early apples’UL higher than late-maturing; UL of guandi and the equator are lower ,UL of luogeng is higher. UL of Apple growing top is largest, followed by Central, at the bottom of the weakest; apples growing in the south and west of the tree, the higher UL value, east of the second, north is the lowest.;As the fruit matured, its UL value decline.
     3. The relationship of fresh apple's ultra weak bioluminescence(UL) properties and quality indicators ,such as sweetness, acidity, hardness, soluble solids content, pH value, is not significant.
     4. During the storage, significantly the ultra weak bioluminescence peaks appear, and UL decreased as the extension of storage time. with the decline in Apples’hardness, PH value decline, the slow increase in soluble solids, UL value also declining UL value can be used to judge Apple fresh grade.
     5. Damage affect Apples’UL value obviously. UL value changed distinctly at damaging moment. UL of damaged Fuji apples changed sharply in storage period, and UL of pressed apples is not significant.The larger area and the deeper of the damages are more remarkable of UL value changes. During storage period, UL of damaged apples reduce rapidly, undamaged apples’UL change slowly. As a new method, UL can distinguish damaged apple from undamaged.
引文
[1] 郡新民,冯建忠,李良瀚.我国苹果生产中存在的问题及对策[J].河北果树,2006,4:2-3.
    [2] 杨振锋,丛佩华,聂继云等.我国苹果产业现状、存在问题及建议[J].北方果树,2006(5):34-36.
    [3] 董一威,基于 CCD 近红外光谱的苹果内部品质检测[D].北京:中国农业大学,2006.
    [4] 汤卫东,刘杰明,陈吉洪. 苹果品质的模糊综合评价[J].食品与药品,2005,7(9):60-64.
    [5] 杨振锋,丛佩华,李静. 苹果内在品质分析[J].中国果树,2002,2:16-17.
    [6] 潘秀娟,屠康. 红富士苹果采后品质变化的破坏与非破坏检测研究[J]. 西北农林科技大学学报,2004,32(9):38-42.
    [7] 张立彬,胡海根,计时鸣等到. 果蔬产品品质无损检测技术的研究进展[J]. 农业工程学报,2005,21(4):176-180.
    [8] 应义斌,刘燕德. 水果内部品质光特性无损检测研究及应用[J]. 浙江大学学报,2003,29(2):125-129.
    [9] 周新仁,丁继高. 果品无损检测技术的研究现状[J]. 食品与发酵工业,2004,30(11):82-84.
    [10] 滕斌,王俊. 国内外瓜果品质的无损检测技术[J].现代化农业,2001,2:2-5.
    [11] 郭文川,朱新华,郭康权. 果品内在品质无损检测技术的研究进展[J]. 农业工程学报,2001,17(5):1-5.
    [12] P. Armstrong, H. R. Zapp, G. K. Brown. Implusive excitation of acousic vibrations in appls for firmness determination, 1990, 32(4):1353-1359.
    [13] Finney, E. E. M echanical. Resonance within Red Delicious apples and its relation to fruits texture. Transactions of the American Society of Agracultural Engineers, 1970,13(2):177-180 .[14] 吴瑞梅,乔振先,严霖元. 鸡蛋新鲜度光特性分级系统的研究[J]. 农机化研究,2005,5:68-70.
    [15] 应义斌,刘燕德. 光纤传感技术在水果内部品质检测中的应用研究[J]. 传感技术学报,2003,2:170-174.
    [16] 毕卫红,付兴虎,王魁荣等. 水果品质近红外检测技术的研究现状与发展. 激光与光电子学进展,2006,43(4):3-7.
    [17] 韩东海,刘新鑫,赵丽丽等. 受损苹果颜色和组织的近红外光谱特性[J].农业机械学报,2003,34(6):112-115.
    [18] Abbott. J. A. G. S. Bachman, N. F. C. h ilders.etc. Sonic techniques for measuring texture of fruits and vegetables. Food Technol, 1968, 22(5):101-102.
    [19] Gunasekaran, S. Paulsen,M. R. Shove, G. C. Opticalmetheds for nondestructive quality evalution of agricultural and biological materials. Journalof agricultural Engineering Research, 1985, 32:209-241
    [20] 胥芳,张立彬,计时鸣等.基于介电特性的水果品质无损检测方法研究[J].浙江工业大学学报,2001,29(3):230-234.
    [21] 宋金亚,张立彬,计时鸣等. 利用介电特性的水果品质无损检测[J]. 无损检测,2003,25(8): 420-422.
    [22] 潘胤飞 基于非线性模式识别的电子鼻技术在苹果分类中的应用[D].苏州: 江苏大学硕士学位论文,2003.
    [23] Nelson S O, Forbes W R , Lawrence K C. Microwave permittivities of fresh fruits and vegetables from 0.2 to 20 GHz[J ] . Trans ASAE.1993,37(1):183-189.
    [24] Kato K. Nondestructive measurement of fruits quality by electrical impedance[J]. Research Report on Agricultural Machinery , KyotoUniversity ,1987,(17):51-68.
    [25] P. Chen,M. J. M cCarthy, R. K. auten. NMR for internal quality evaluation of fruits and vegetables. American Society of A gricultural Engineers,1989,32 (5):1747-1753.
    [26] 何东健.农产品分光反射特性及近红外图像处理在农业中的应用.农业工程报,1996,12(4):80-84.
    [27] Schaare P N , Fraser D G. Comparison of reflectance interactance and transmission modes of visiblenear infrared spectroscopy forme assuring internal properties of kiwi fruit[J]. Post harvest Biology and Technology,2000, 20 :175-184.
    [28] Lu R,Ariana D.A Nearinfrared sensing technique for measuring internal quality of apple fruit[J].Trans of the ASAE,2002,18(5):585-590.
    [29] 邹小波,吴守一,方如明. 电子鼻判别挥发性气体的试验研究[J].江苏理工大学学报2001,22(2):1-4.
    [30] Gardner J W, Bartlett P N ( Editors ). Sensors and Senso ry systems for an electronic nose [M]. London:Kluwer Academic Publishers, 1994,NA TO A SI Series.Series E: Applied Sciences, vol. 212.
    [31] Chen P, Ruiz, Altisent, Barreiro P. Effects of impacting mass on firmness sensing of fruits[J ]. Transact ions of the ASAE, 1996,39(3):1019-1023.
    [32] F.A.Popp, K.H.Li, Q.Cu. Recent advances in biophoton research and its applications[M]. Singapore: World Scientific Publishing Co. 1992.
    [33] 张仲伦.微弱发光分析技术应用实例[J]. 生物化学与生物物理进展,2000,27(1):102-104.
    [34] 程海鹏,薛建华,王君晖. 生物中的超微弱发光[J]. 生物学通报,1999,34(11):15-17.
    [35] 刘流,郭红英. 生物的微弱发光及其应用[J]. 生物学教学,2003,28(8):6-7.
    [36] 强红,王香凤,朱若华等. 生物超微弱发光现象及其应用[J]. 化学教育,2006,2:6-9.
    [37] 李勤. 生物系统的发光原理及其应用[J]. 生命科学仪器,2004,2(4):33-37.
    [38] 李淑丽,郑新亮,任兆玉. 生物超微弱发光研究进展[J]. 激光杂志,2004,25(4):4-6.
    [39] 李淑丽. 番茄成熟过程中超微弱发光研究[D]. 西安:西北大学,2005.
    [40] 候仙慧,廖祥儒,李颖等. 苋菜种子萌发过程的超微弱发光及其机理研究. 种子,2004,23(7):24-27.
    [41] 曹晓兵,李光,廖祥儒等. 盐胁迫下绿豆幼苗的超微弱发光. 热带亚热带植物学报刊,2004,12(3):261-264.
    [42] 刘高峰,杨洪强,张伟. 水杨酸对湖北海棠活性氧代谢及超微弱发光的影响[J]. 园艺学报,2006,33(1):118-121.
    [43] 程海鹏,王君晖,池浩超等. 豌豆种子萌发过程中超微弱发光的研究[J].浙江大学学报,2001,28(6).
    [44] 吕家根,占达东,王周平. 酸雨胁迫下小麦微弱延迟发光及其生理、生态变化相关性研究[J].化学学报,2003,61(5):760-764.
    [45] 刘红梅,廖祥儒,吴立峰等. 热休克对小麦未成熟种子萌发、生物发光和抗氧化酶活性的影响[J]. 食品与生物技术学报, 2006,25(3):75-78.
    [46] 陈文利,唐永红,邢达. 新旧及不同抗性的水稻种子吸胀初期超微弱发光特性的观测[J].华南师范大学学报,2001,2:50-54.
    [47] 胡献明,潘建伟, 陈虹等. 铝胁迫诱导大麦根尖细胞超微弱发光的变化[J].浙江大学学报, 2002,28(4):383~386.
    [48] 杨起简,周禾,С. И. Погосян.不同钠盐胁迫对豌豆幼苗超弱发光的影响[J].核农学报,2003,17(2):111-114.
    [49] 张菊平,张兴志,巩振辉. 超微弱发光在蔬菜研究中的应用[J]. 中国农学通报,2006,22(1):220-222.
    [50] 邹飞,湛学军,章涛等. 肿瘤患者血清超微弱发光的研究[J].江西医学实验科学,2001,19(2):90.
    [51] 马世荣,惠延平,郭双平等. 人肝癌 HepG2 细胞超微弱发光的观察[J]. 医学研究生学报,2006,2(19):100-103.
    [52] 王成龙,范多旺,邢达等. 植物光诱导延迟发光介导的酸性环境污染监测[J]. 光电子·激光,2006,17(3):337-342.
    [53] 张世民,傅俊杰,包劲松. 超微弱发光分析法在辐照糖类检测中的应用[J].核农学报,2003, 17(3):231-232.
    [54] 张世民,傅俊杰,包劲松. 辐照后食品超微弱发光性质的变化[J].核农学报,2005,19(2):105-108.
    [55] 张新华,李富军,杨洪强等.苹果成熟过程中超弱发光强度与果实跃变的关系[J].农业机械学报,2004,35(6):215-217.
    [56] 张新华,杨洪强,李富军. 苹果果实超弱发光、乙烯释放和呼吸跃变对 1-MCP 的响应[J]. 中国食品学报,2006,6(2):63-66
    [57]Triglia A,Ismalfa G,Musumecl F,et al. Delayed luminescence as an indicator of tomato fruit quality[J]. Joumal of Food Science,1998,63(3):512-515.
    [58] 赵红霞,张守勤,周丰昆等. 鸡蛋超弱发光与其新鲜程度的相关分析[J]. 农业工程学报,2004,20(2):177-180.
    [59] 马瑜,俞信,王苏生,李勤. 超微弱光子图像的相关检测及计算机模拟[J]. 光学学报,2001,21(10):1194-1198.
    [60] 马瑜,俞信. 光子图像统计处理[J]. 光学学报,2002,22(4):422-426.
    [61] 王苏生. 生物光子图像的探测概率和极限[J].光学学报,1999,19(7):920-923.
    [62] 王苏生. 显微光子计数成像系统及其应用[J].光学学报,2000,20(8):1072-1076.
    [63] 陈江丽,李晔,夏靖波. 微波场影响生物体超弱发光的研究[J]. 空军工程大学报,2002,3(2):91-94.
    [64] 陈江丽,陆治国.蒜的延迟发光光电子[J].激光,1999,10(4):365-367.
    [65] 李光,杨海莲,陈静伟等. 温度对叶片延迟发光的影响[J]. 河北大学学报,2005,25(1):24-28.
    [66] 杨晓梅,德庆措姆. 低温引起苹果品质及有机物的变化[J].西藏农业科技,2003,24(2):17-19.
    [67] 屠康,邵兴锋,赵艺泽. 采后热空气处理对金冠苹果后熟衰老及病害的影响[J].果树学报,2006,23(4):562-567.
    [68] 雷琴,苹果成熟过程中品质变化特性研究[D].杨凌:西北农林科技大学,2006.
    [69] 杨起简,周禾,鲍超等. 植物幼苗原发性超弱光子成像的研究[J].激光生物报,2002,11(3):161-163.
    [70] 张新萍,岳霞丽,陈建军等. 水华鱼腥藻超弱发光初探[J]. 湖北农业科学,2005,2:13-14.
    [71] 李光,陈静伟,杨海莲. 双氧水对叶片延迟发光的影响[J]. 河北大学学报,2006,26(4):366-372.
    [72] 王维江,邢达,谭石慈等. 红背桂叶片的延迟发光研究[J].量子电子学报,2000,17(4):310-314.
    [73] 何湘,吴平,高思峰. 石蒜根部延迟发光特性的研究[J].江西科学,2005,23(5):554-556.
    [74] 张新华,杨洪强. 植物叶绿体和线粒体的超微弱发光[J].植物生理学通讯,2004,40(1):111-114.
    [75] 谭石慈,邢达,唐永红等. 植物叶片超微弱发光光谱研究[J].光子学报 2000,29(11):961-965.
    [76] 宋吉吉,不同光质照射对富士苹果果实着色影响及相关着色技术研究[D].北京:中国农业大学,2006.
    [77] 鲍士旦.土壤农化分析[M].北京:中国农业出版社,2005.
    [78] 郭红利.猕猴桃的电学特性与无损检测技术的研究[D].杨凌:西北农林科技大学,2004.
    [79] 鲁玉妙,高华,赵政阳等. 粉红女士苹果品质特性分析评价[J].果树学报,2003,20(6):503-505.
    [80] 张新华,杨洪强. 植物的超微弱发光[J]. 山东农业大学学报,2003,34(4):605-608.
    [81] 王维江,邢达,谭石慈等. 植物超弱发光的探测[J].光电子·激光 2000,11(4):427-430.
    [82] 列光华,赵鹏飞,张信明等. 激光微束技术、生物超微弱发光与生物生命奥秘[J]. 青海师范大学学报,2003,1:78-81.
    [83] 郭文川,朱新华,郭康权.损伤对苹果电参数值的影响[J].农业机械学报,2006,37(8):133-135.
    [84] 徐澍敏,于勇,王俊.机械损伤苹果 CT 值的试验研究[J].农业机械学报,2006, 37(6):83-85.
    [85] 高晓阳, Paul Heinem ann, Joseph Irudayaraj. 苹果擦伤拉曼光谱无损检测虚拟系统研究[J].农业工程学报,2005,21(3):130-133.
    [86] 卢立新. 跌落损伤脆值及损伤边界[J]. 包装工程,2005,26(6):1-4.
    [87] 高晓阳, Paul Heinem ann, Joseph Irudayaraj. 基于的苹果擦伤分级系统设计[J].农业机械学报,2006,37(4):152-154.
    [88] 韩东海,刘新鑫,鲁超等.苹果内部褐变的光学无损伤检测研究[J].农业机械学报,2006,37(6):86-88.
    [89] 田心棣(美).膜的光生物物理与光化学[M]. 北京:科学出版社,1998.
    [90] 孙一源,余登苑. 农业生物力学及农业生物电磁学[M].北京:中国农业出版社,1996.
    [91] A. Planner a, D. Frackowiak a,. j. Miyake b .Delayed luminescence of some components of optoelectronic devices[J]. Thin Solid Films,1997,295: 224-227
    [92] Marina Montia, Alexis Zrimecb, Alfred Berana,etc. Delayed luminescence of Prorocentrum minimum under controlled conditions. Harmful Algae[J].2005,4:643–650.
    [93] Yu Yan, Fritz-Albert Popp, Sibylle Sigrist,etc. Further analysis of delayed luminescence of plants. Journal of Photochemistry and Photobiology[J].2005,78:235–244.