摘要
番茄中类胡萝卜素是番茄果实呈现黄色、橙红色至红色的原因。不少类胡萝卜素具有维生素A原和抗癌活性，是食物中不可缺少的成分。
本研究用高效液相色谱法同时测定了转反义ACS(ACC 合酶)基因番茄果实和普通番茄果实盛花后不同成熟时期的α-胡萝卜素、β-胡萝卜素、番茄红素、叶黄素等类胡萝卜素含量，结果表明两者的β-胡萝卜素和叶黄素无明显差异，番茄红素含量存在显著差异，转反义ACS基因番茄果实中番茄红素在花后50天后才有检出，含量仅为同时期普通对照番茄的1／56。利用外源乙烯对花后35天转反义ACS基因番茄处理后，类胡萝卜素合成增加，尤其是番茄红素迅速累积，用外源乙烯处理6天后基本达到花后55天普通番茄中番茄红素含量。
由此推断：类胡萝卜素在生物合成途径中，转反义ACS基因番茄果实中β-胡萝卜素和叶黄素生物合成不受抑制，而番茄红素合成受到抑制。施加外源乙烯后，促进了类胡萝卜素的生物合成。
结果表明，由于转反义ACS基因番茄果实中，乙烯合成受到抑制，番茄红素合成迟滞；施加外源乙烯后，乙烯对转反义ACS基因番茄果实中番茄红素、叶黄素和β-胡萝卜素等类胡萝卜素的生物合成具有促进作用，尤其是对番茄红素的累积作用极其显著。番茄红素的生物合成是依赖于乙烯的。
Carotenoids can make tomato to show color as yellow, orange and red. Most of posses activity of vitamin A and anti-cancer, they are also the necessary of food.
This paper detected the a - carotene, β-carotene, lycopene and xanthophyll in wild and antisense-ACS (ACC synthase) tomato fruit using High Performance Liquid Chromatography (HPLC) during ripening. The result suggested that there are significant different between wild and transgenic tomato about the amount of lycopene. The lycopene synthesize was detected in antisense-ACS tomato fruit 50-days after anthesis, and the content was only 1/56 compare with the wild tomato fruit at the same period. Exotic ethylene treating can promote carotenoids synthesize and especially accumulate lycopene in tomato fruit after pollen 35-days. The content of lycopene in antisense-ACS tomato fruit treated with exotic ethylene 6 days later reached about the same numerical value.
We can concluded: during the biosynthesis of carotenoids,β-carotene and xanthophylls were not controlled while lycopene was blocked in antisense-ACS tomato fruit. The biosynthesis of Carotenoids can be promoted by treatment with exotic ethylene in antisense-ACS tomato fruit.
The result show that lycopene synthesize was deferred in transgenic tomato fruit. After treated ethylene lycopene accumulation was increased in transgenic tomato fruit. The biosynthesis of Carotenoids can be promoted by treatment with exotic ethylene in antisense-ACS tomato fruit. The lycopene was significantly promoted. That is to say, the biosynthesis of lycopene depend on ethylene.
引文
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