摘要
目的：建立清醒状态下大鼠高胰岛素-正常血糖钳夹术，并探讨短期输注乳化脂肪和手术后麻醉状态对大鼠胰岛素敏感性的影响。方法：采用颈动、静脉插管术。大鼠按有无输注乳化脂肪随机分为两组，先后进行麻醉状态和清醒状态高胰岛素-正常血糖钳夹术。结果：短期输注乳化脂肪使胰岛素敏感性降低30%（Ｐ<0.001）；手术后麻醉状态使胰岛素敏感性降低58%（Ｐ<0.001）；以上二因素对胰岛素敏感性的影响存在相减效应（Ｐ<0.001）。结论：给大鼠短期输注乳化脂肪会导致胰岛素抵抗。大鼠高胰岛素-正常血糖钳夹术宜在清醒状态下进行。
Objective：To establish hyperinsulinemic euglycemic clamp technique in conscious rat, and to explore the effect of anesthesia and acute infusion of lipid on insulin sensitivity of rats. Methods：Rats were catheterized in the right jugular vein and left carotid artery after divided into two groups, and then undergoing a hyperinsulinemic- euglycemic clamp with or without infusion of lipid (Intralipid) and in unconscious and conscious state. Results：Infusion of lipid resulted in a decrease of insulin sensitivity by 30% (P<0.001 vs. control). In unconscious rats, the insulin sensitivity was decreased by 58% (P<0.001 vs. conscious). There was an antagonistic effect between infusion of lipid and anesthesia on insulin sensitivity of rats (P<0.001). Conclusion：The increase plasma free fatty acid induces insulin resistance. It would be better to carry out hyperinsulinemic-euglycemic clamp under conscious state in rats.
引文
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