摘要
本研究以京海黄鸡为研究对象,以类胰岛素生长因子2及其受体基因为候选基因,采用PCR-SSCP方法检测候选基因的单核苷酸多态性,探讨不同基因型以及基因型组合与京海黄鸡主要生产性能的相关性。
1.本研究中,对IGF2基因设计了7对引物,其中引物P1、P2、P3、P5、P7五对引物检测到IGF2基因6个突变位点(G64A、C173A、G6707C、T7506C、G7641A、G8120A),对IGF2R基因设计了7对引物,仅引物R6检测到IGF2R基因外显子4存在T15262A突变。
2.本研究检测到的突变位点中,除P3外均属于中度多态位点(0.50>PIC>0.25),P1、P2位点符合Hardy-Weinberg平衡(P>0.05),P3位点显著偏离Hardy-Weinberg平衡(P<0.05),P5、P7、R6位点极显著偏离Hardy-Weinberg平衡(P<0.01)。
3. IGF2基因P1、P2、P3、P5和P7引物不同的基因型与京海黄鸡的生产性能的统计分析结果显示,C2C2型个体12周龄体重和16周龄体重极显著大于C1C2型个体(P<0.01)。
P5位点D1D2型个体4周龄体重显著大于D1D1型(P<0.05),且12周龄极显著大于D1D1型个体(P<0.01)。
P7位点E1E2型个体12周龄体重显著大于E1E1型个体(P<0.05),蛋壳强度极显著小于E1E1型个体(P<0.01)。
4. IGF2R基因R6引物不同基因型与京海黄生产性能的统计分析结果显示,G1G2型个体蛋壳强度显著大于G2G2型个体(P<0.05)。
5. IGF2基因P2、P5、P7三个位点的单倍型组合分析显示,两两位点间存在一定的连锁,但不属于强连锁平衡状态。三个位点组合基因型与生产性能相关分析显示,B1B2/D1D2/E1E2和B1B2/D1D2/E1E1组合基因型在生长、产蛋及蛋壳质量性状方面都具有较高的性能。
In this research, IGF2 gene and IGF2R gene were selected as candidate genes affecting correlate traits in Jinghai yellow chicken. PCR-SSCP and DNA sequencing were used to detect the SNPs in candidate genes in order to research the correlation between different genotypes and the productive traits.
1. According to the sequence of IGF2 gene, seven primers were designed and six mutations (G64A, C173A, G6707C, T7506C, G7641A, and G8120A) were found for primers P1, P2, P3, P5 and P7. According to the sequence of IGF2R gene, seven primers were designed. Three genotypes were found for primer R6 of the exon 4, and the mutation T15262A was detected.
2. The mutations were moderately polymorphics except the P3 loci. Statistic results showed that the loci P1 and P2 were in Hardy-Weinberg Equilibrium status (HWE). The loci P3, P5, P7 and R6 deviated from Hardy-Weinberg Equilibrium status.
3. The Least Square Means analysis showed C2C2 had higher 12-week-weight and 16-week-weight than C1C2 (P<0.01). D1D2 had higher 4-week-weight than D1D1 (P<0.05) and also had higher 12-week-weight than D1D1 (P<0.01). E1E2 had higher 12-week-weight than E1E1 (P<0.05), while the difference of the egg shape index between E1E1 and E1E2 was significant (P<0.01). For locus R6 of the IGF2R gene, G1G2 had higher eggshell strength than G2G2 (P<0.05) .
4. The analysis of haplotype combinations showed that P2, P5, P7 had some linkage but not at the strong linkage disequilibrium. The analysis between com-genotypes and production performance showed that B1B2/D1D2/E1E2 and B1B2/D1D2/E1E1 had the high production performance.
引文
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