摘要
目的:检测转化生长因子β1及Smad7蛋白在扁平苔藓病变组织中的表达及分布,探讨其在扁平苔藓发病机制中的作用。
方法:采用免疫组化二步法,分别用转化生长因子β1兔抗人多克隆抗体和Smad7蛋白兔抗人多克隆抗体检测30例扁平苔藓皮损及30例正常皮肤中转化生长因子β1及Smad7蛋白的表达及分布情况,利用Leica手动显微镜及数码相机采集图像,Qwin软件(v2.3,Leica Inc.,德国)分析阳性区域积分光密度值,以积分光密度值大小代表阳性产物表达的多少,分别对实验组及对照组转化生长因子β1及Smad7蛋白表达的差异进行统计学分析。
结果:正常皮肤中转化生长因子β1在绝大多数表皮基底细胞有表达,扁平苔藓皮损中转化生长因子β1均为阴性表达,仅在真皮浅层个别浸润淋巴细胞中偶见表达。扁平苔藓皮损中转化生长因子β1的表达低于对照组,差异有统计学意义(t'=7.380,P＜0.05)。Smad7蛋白在扁平苔藓皮损中表皮内为强阳性表达,表皮着色为全层连续着色或灶状着色,以基底细胞、棘细胞及大部分颗粒层细胞胞浆着色为主;真皮浅层浸润的淋巴细胞为阴性表达。Smad7蛋白在正常皮肤表皮及皮下均为阴性表达。二者差异有统计学意义(t'=10.088,P＜0.05)。
结论:转化生长因子β1在扁平苔藓病变组织中表达缺失,Smad7蛋白在扁平苔藓病变组织中表达增高,提示二者在扁平苔藓的发病中发挥一定作用,研究结果支持扁平苔藓的发病与免疫异常相关。
Objective:This study aim to evaluate expression of transforming growth factorβ1 and Smad7 protein in lichen planus,and investigate their possible role in pathogenesis of lichen planus.
Methods:The immunohistochenmical 2-step was used to detect the expreesion of transforming growth factorβ1 and Smad7 protein with the transforming growth factorβ1 rabbit anti human polyclonal antibody and the Smad7 protein rabbit anti human polyclonal antibody in 30 cases of lichen planus and 25 cases of normal skin. Leica manual microscope and Qwin image analysis system(v2.3,Leica Inc.,German) were used to analysis the positive results with integral optical density,then measure the positive product with its integral optical density,then analyze the differences of transforming growth factorβ1 and Smad7 protein expressed in the experimental group and the control group by principle of statistics.
Results:The study showed that the expreesion of transforming growth factorβ1 in normal skin was in majority basal cells of epidermis;while in lichen planus the expreesion of transforming growth factorβ1 was negative.The expreesion of transforming growth factorβ1 in lichen planus was lower than in normal skin(t' =7.380,P＜0.05).The Smad7-immunohistochemistry was seen significantly in diseased region of lichen planus,the coloring of epidermis was continuous or focus-form of full-thickness,the positive coloring was mostly in basal cells、acanthocyte and mainly granulosa cells,the expression of Smad7 protein in the infiltrative lymphocytes which were in the superficial layer of dermis was negative;while the expression of Smad7 protein in the epidermis and dermis of normal skin was negative.The difference of the two groups was significantly(t'=10.088, P＜0.05).
Conclusion:The absence expression of transforming growth factorβ1 and the positive expression of Smad7 protein in lichen planus prompt that may act an certain role in the pathogenesis of lichen planus.The study result support that the pathogenesis of lichen planus was related to the immunologic abnormality.
引文
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