摘要
理想支架材料的研究和运用是关乎骨组织工程成功与否的重要因素。支架材料在骨组织工程中的作用，是为新生骨组织提供生长所需的三维空间和力学支持。蚕丝丝素蛋白属于一种天然的纤维蛋白。丝素蛋白具有优良的生物相容性和力学性能，可以缓慢降解，在医药和生物技术等方面已有广泛应用、并取得了一些可喜的进展。但是现阶段将蚕丝蛋白应用于骨组织工程的相关研究还比较单一，主要停留在合成复合材料的方法性研究。所合成材料的结构特性还不能满足骨组织工程支架材料或骨替代材料的要求。
本论文旨在研究丝素蛋白的矿化行为，并通过仿生矿化来制备三维多孔非编织丝素蛋白／羟基磷灰石支架，用于骨组织工程。
首先，研究了丝素蛋白膜的矿化行为。将丝素膜浸泡在1.5×SBF、SCS和ASS三种矿化液中，比较不同的矿化处理过程对仿生矿化的影响。另外，SF膜经饱和Ca(OH)_2预矿化处理后，再浸泡于3种矿化液中，观察预矿化对整个仿生矿化的影响。样本经SEM、XRD、EDX、FTIR检测证明：SF膜可以通过1.5×SBF，SCS，ASS
An ideal scaffold material is one of an important factors leading to the success in the field of bone tissue engineering. The scaffold materials provide mechanical stability and maintain a three-dimensional space for the formation of new bone tissue with appropriate structure. Silk fibroin (SF) is a natural fibrous protein and has been proved as a promising material for a number of biomedical and biotechnological applications because of its excellent biological compatibility, mechanical strength and biodegradation. Mimicking and fabricating the hierarchical organization would be one of the future ways to improve the biocompatibility of calcium phosphate/organics composites and to simulate the biological bone from its micro/meso-structure.
The Ph.D thesis is focused on the study of the mineralization behavior of fibroin in order to fabricate three dimensional porous
引文
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