摘要
目的:研究P57kip2和Maspin在病理性瘢痕组织中的表达情况及相互关系,探讨它们在病理性瘢痕形成中的作用及机制。
方法:应用免疫组化SP法结合计算机病理图像分析和逆转录聚合酶链反应(RT-PCR)检测正常皮肤、成熟瘢痕、增生性瘢痕和瘢痕疙瘩组织中P57kip2和Maspin的表达和分布并对其表达进行统计学分析。
结果:病理性瘢痕组织中P57kip2蛋白的表达定位于成纤维细胞细胞核内,且P57kip2蛋白及mRNA的表达减少,与正常皮肤、成熟瘢痕对照组比较差异有统计学意义(P<0.005)。瘢痕疙瘩与增生性瘢痕之间,P57kip2蛋白及mRNA的表达差别均无统计学意义(P>0.05)。病理性瘢痕组织中Maspin蛋白的表达定位于成纤维细胞的细胞质和细胞核内,且Maspin蛋白及mRNA的表达减少,与正常皮肤、成熟瘢痕对照组比较差异有统计学意义(P<0.005)。瘢痕疙瘩与增生性瘢痕之间, Maspin蛋白及mRNA的表达差别无统计学意义(P>0.005)。P57kip2与Maspin蛋白表达存在明显正相关(P<0.01)。
结论:P57kip2与Maspin在病理性瘢痕组织中均表达减少,是病理性瘢痕相关基因。P57kip2、Maspin在病理性瘢痕中存在明显正相关。由此推测P57kip2和Maspin的表达减少和相关性可能是病理性瘢痕的形成机制之一。P57kip2和Maspin可能通过成纤维细胞在病理性瘢痕的形成过程中发挥着重要作用。
Objective To study the expression of P57kip2 and Maspin in the pathological scar and their probable role in the pathogenesis of abnormal scars.
Methods Immunohistochemistry integrated image analysis and reverse transcriptionpolymerase chain reaction (RT-RCR) were performed to detect the expression and distribution of P57kip2 and Maspin in hypertrophic scar, keloid, mature scar and normal skin. Statistics was used to analyze the datas of this expression. Results The expression of P57kip2 protein fixing to fibroblast intranuclear in abnormal scar ,as well the expression of P57kip2 protein and P57kip2 mRNA increased (P<0.005)and their expression was not statistically defferent between the hypertrophic scar and keloid(P>0.05). The expression of Maspin protein fixing to fibroblast cytoplasm and intranuclear in abnormal scar , as well the level of Maspin protein and Maspin mRNA expression was not statistically defferent between the hypertrophic scar and keloid(P>0.05), while they were all remarkably significant in comparison between normal control group and abnormal scar(P<0.005). There was positive correlation existing between P57kip2 protein and Maspin protein expression(P<0.01).
Conclusion The result indicated that the expression of P57kip2 and Maspin was decrease in abnormal scar, they were a cicatrix specific gene. P57kip2 and Maspin are tightly associated with the development of abnormal scar. P57kip2 has a positive correlation with Maspin in abnormal scar, which suggested that the expression reduce of P57kip2 and Maspin as well as the dependablity between P57kip2 and Maspin maybe one mechanism of abnormal scar’s formation. P57kip2 and Maspin may play a major effect on the formation process of abnormal scar by fibroblast.
引文
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