摘要
目的：研究Hedgehog信号通路中shh,ptch和smo蛋白过表达在乳腺浸润性导管癌及导管内原位癌中的临床病理学意义。
方法：采用免疫组化EnVision方法检测111例浸润性导管癌、40例导管内原位癌和21例正常乳腺导管上皮组织芯片中shh,ptch和smo蛋白的表达情况,并分析其相关性。
结果：shh、ptch和smo蛋白在正常导管上皮中表达阴性或弱阳性,但在浸润性导管癌(shh:74.8%,ptch:68.5%,smo:65.8%)和导管内原位癌(shh:100%,ptch:100%,smo：90%)中阳性表达率明显增高,差异有统计学意义(均P<0.001)。乳腺导管癌中shh蛋白的表达率在Ki67和c-erbB2阳性组明显高于阴性组(均P<0.05)；而且与淋巴结转移相关(P<0.05)。此外,shh、ptch和smo蛋白表达与乳腺癌患者的年龄、ER、PR、p53蛋白的表达及组织学分级和临床分期等特征均无显著性关系。Spearman相关分析显示,在乳腺癌组织中shh的表达分别与ptch(r=0.55，P<0.01)、smo(r=0.58,P<0.01)的表达呈正相关；而且ptch的表达又与smo的表达呈正相关(r=0.495,P<0.01)。
结论：shh、ptch和smo蛋白表达检测可以作为乳腺癌的早期辅助诊断指标,而且shh对判定乳腺癌的预后有一定的作用。
Objective:To investigate the clinicopathological significance of shh, ptch and smo protein overexpression in breast invasive ductal carcinoma and ductal carcinoma in situ (DCIS).
Methods:The expression of shh, ptch and smo proteins was detected by EnVision method of immunohistochemistry in tissue-microarray making from 111 cases of breast invasive ductal carcinoma,40 cases of DCIS,and 21 cases of normal breast ductal epithelium tissues, and their correlations were analyzed.
Results:The immunohistochemical staining showed that the expressions of shh,ptch and smo in normal ductal epithelium were completely absent or rare, but significantly higher in invasive ductal carcinoma (shh:74.8%, ptch:68.5%,smo:65.8%) and DCIS (shh:100%, ptch:100%, smo:90%) than in normal ductal epithelium (all P<0.001).In ductal carcinoma, shh protein expression rate was significantly higher in both of Ki67 and c-erbB2 positive groups than negative groups (all P<0.05), and was correlated to lymph node metastasis (P<0.05).Whereas the expression of shh, ptch and smo in breast cancer showed no correlation to patients'age, ER, PR, p53 protein expression, tissue grade, and clinical stage. Spearman correlation analysis showed that shh expression was positively correlated to ptch (r=0.55,P<0.01)and smo (r=0.58,P<0.01)expression,respectively; and ptch expression was positively correlated to smo expression (r=0.495,P<0.01).
Conclusion:shh,ptch and smo protein overexpression might be a helpful marker for the early diagnosis of breast cancer, and the detection of shh might be helpful for indicating the prognosis of the breast cancer.
引文
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