甜菜碱对低温胁迫下黄芩幼苗生理特性的影响
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摘要
为缓解黄芩的冷害,探讨外源甜菜碱(GB)对低温胁迫下幼苗生理特性的影响,以商洛黄芩为试验材料,在4℃低温胁迫下,研究叶面喷施GB对黄芩幼苗的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、丙二醛(MDA)、可溶性蛋白和叶绿素含量、相对电导率等生理指标的影响。结果表明:施加浓度为0.6g/L外源GB能有效提高黄芩的抗寒性,黄芩幼苗叶片SOD、POD和CAT活性分别为低温蒸馏水对照的1.502倍、2.031倍和2.362倍,且都略低于常温蒸馏水对照;总叶绿素含量相对于低温蒸馏水对照高68%;可溶性蛋白含量较低温蒸馏水对照高178%;随着甜菜碱处理浓度升高,低温胁迫下黄芩的相对电导率和丙二醛含量呈先降后升趋势,且均在GB浓度为0.6g/L时含量达到最低,比低温蒸馏水对照分别下降50%和56%。因此甜菜碱喷洒处理能明显缓解冷害,且在GB浓度为0.6g/L效果最好。
The effects of glycine betaine(GB)on leaf SOD,POD and CAT activity,MDA,soluble protein and chlorophyll content and relative conductance rate of Scutellaria Baicalensis seedlings were studied by apot test under 4℃ low temperature stress to discuss the effect of exogenous GB on physiological property of S.Baicalensis seedlings under low temperature stress for relieving cold damage to S.Baicalensis seedlings.Result:Spraying 0.6 g/L GB can effectively improve cold resistance of S.Baicalensis seedlings and the leaf SOD,POD and CAT activity of S.Baicalensis seedlings is 1.502,2.031 and 2.362 times that of CK_2(distilled water+low temperature),slightly lower than CK1(distilled water+ normal temperature).Total chlorophyll and soluble protein content in leaves of S.Baicalensis seedlings sprayed with 0.6 g/L GB is 68% and 178% higher than CK_2 respectively.The leaf relative conductance rate and MDA content of S.Baicalensis seedlings both show the rising firstly and then declining trend with increase of GB concentration under low temperature stress.The leaf relative conductance rate and MDA content of S.Baicalensis seedlings both reach the lowest when GB concentration is 0.6 g/L,and are 50% and 56%lower than CK_2 separately.In conclusion,spraying GB can relieve cold damage to S.Baicalensis seedlings and the optimum GB concentration is 0.6 g/L.
The effects of glycine betaine(GB)on leaf SOD,POD and CAT activity,MDA,soluble protein and chlorophyll content and relative conductance rate of Scutellaria Baicalensis seedlings were studied by apot test under 4℃ low temperature stress to discuss the effect of exogenous GB on physiological property of S.Baicalensis seedlings under low temperature stress for relieving cold damage to S.Baicalensis seedlings.Result:Spraying 0.6 g/L GB can effectively improve cold resistance of S.Baicalensis seedlings and the leaf SOD,POD and CAT activity of S.Baicalensis seedlings is 1.502,2.031 and 2.362 times that of CK_2(distilled water+low temperature),slightly lower than CK1(distilled water+ normal temperature).Total chlorophyll and soluble protein content in leaves of S.Baicalensis seedlings sprayed with 0.6 g/L GB is 68% and 178% higher than CK_2 respectively.The leaf relative conductance rate and MDA content of S.Baicalensis seedlings both show the rising firstly and then declining trend with increase of GB concentration under low temperature stress.The leaf relative conductance rate and MDA content of S.Baicalensis seedlings both reach the lowest when GB concentration is 0.6 g/L,and are 50% and 56%lower than CK_2 separately.In conclusion,spraying GB can relieve cold damage to S.Baicalensis seedlings and the optimum GB concentration is 0.6 g/L.
引文
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[23]代勋,李忠光,龚明.赤霉素、钙和甜菜碱对小桐子种子萌发及幼苗抗低温和干旱的影响[J].植物科学学报,2012,30(2):204-205.
[24]李永华,邹琦.植物体内甜菜碱合成相关酶的基因工程[J].植物生理学通讯,2002,38(5):100-104.
[25]王贵平,薛晓敏,路超,等.甜菜碱提高植物抗逆性的作用及其作用机理[J].江西农业学报,2014,26(8):22-26.
[26]王素平,郭世学,胡晓辉,等.盐胁迫对黄瓜幼苗叶片光合色素含量的影响[J].江西农业大学学报,2006,28(1):32-38.
[27]赵秋月,张广臣.碱性盐胁迫对3种番茄根系活力和光合色素的影响[J].江苏农业科学,2015,43(11):219-223.
[28]李新梅,孙丙耀,谈建中.甜菜碱与植物抗逆性关系的研究进展[J].农业科学研究,2006,27(3):66-68.
[2]张晓虎.陕西秦岭“五大商药”施肥技术的研究综述[J].陕西农业科学,2010(4):94-97.
[3]华智锐,李小玲.PEG处理对商洛黄芩种子萌发和幼苗生长的影响[J].种子,2011,30(1):101-104.
[4]何丽丹,刘广明,杨劲松,等.根施甜菜碱缓解黑麦草幼苗盐胁迫效应研究[J].土壤学报,2013,50(5):105-108.
[5]隽加香.低温胁迫下番茄植株光合及呼吸代谢特性的研究[D].哈尔滨:东北农业大学,2015.
[6]PRASAD K V S K,SARADIHI P P.Enhanced tolerance to photo inhibition to transgenic plantsthrough targeting of glycinebetaine biosynthegenic into the chloropiasts[J].Plant Science,2004,166(5):1197-1212.
[7]杨洪兵.甜菜碱对盐胁迫下荞麦幼苗生理特性的影响研究[J].贵州农业科学,2013,41(8):51-53.
[8]韩多红,李善家,王恩军,等.外源钙对盐胁迫下黑果枸杞种子萌发和幼苗生理特性的影响[J].中国中药杂志,2014,7(1):68-70.
[9]梁小红,宋峥,徐广臣,等.外源甜菜碱对低温胁迫下结缕草生理特性的影响研究[J].草业学报,2015,9(24):181-188.
[10]李明,王根轩.干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响[J].生态学报,2002,22(4):503-507.
[11]REN J,HUANG Z L,ZENG L X,et al.A review of physiological reaction mechanism of plants exposed to low temperature stress[J].World Forestry Research,2014,26(6):15-20.
[12]范春丽,曲金柱.甜菜碱浸种对盐胁迫下石榴种子萌发及幼苗生理的影响[J].中国果树,2016,45(1):86-90.
[13]李欣,魏朔南.黄芩的生物学研究进展[J].中国野生植物资源,2006,25(6):11-15.
[14]苏文,潘李茂,富华孙.甜菜碱对低温胁迫下香蕉幼苗细胞膜保护酶活性的影响[J].广西农业科学,2005,4(2):21-24.
[15]李阳,董然,刘在民,等.甜菜碱对低温胁迫下黄瓜幼苗耐冷性相关指标的影响[J].江苏农业科学,2005,7(4):217-219.
[16]梁小红,宋峥,徐广臣,等.外源甜菜碱对低温胁迫下结缕草生理特性的影响研究[J].草业学报,2015,9(24):181-188.
[17]卫丹丹.低温胁迫下甜菜碱对番茄叶片光合作用的保护机制[D].泰安:山东农业大学,2016.
[18]张志良,瞿伟菁.植物生理学实验指导[M].3版.北京:高等教育出版社,2003:48-50.
[19]李合生.植物生理生化实验原理与技术[M].北京:高等教育出版社,2000:167-169.
[20]郝再彬,苍晶,徐仲.植物生理实验[M].哈尔滨:哈尔滨工业大学出版社,2004:110-112.
[21]高俊风.植物生理学实验指导[M].北京:高等教育出版社,2006:213-215.
[22]赵世杰,史国安,董新纯.植物生理学实验指导[M].北京:中国农业科学技术出版社,2002:98-102.
[23]代勋,李忠光,龚明.赤霉素、钙和甜菜碱对小桐子种子萌发及幼苗抗低温和干旱的影响[J].植物科学学报,2012,30(2):204-205.
[24]李永华,邹琦.植物体内甜菜碱合成相关酶的基因工程[J].植物生理学通讯,2002,38(5):100-104.
[25]王贵平,薛晓敏,路超,等.甜菜碱提高植物抗逆性的作用及其作用机理[J].江西农业学报,2014,26(8):22-26.
[26]王素平,郭世学,胡晓辉,等.盐胁迫对黄瓜幼苗叶片光合色素含量的影响[J].江西农业大学学报,2006,28(1):32-38.
[27]赵秋月,张广臣.碱性盐胁迫对3种番茄根系活力和光合色素的影响[J].江苏农业科学,2015,43(11):219-223.
[28]李新梅,孙丙耀,谈建中.甜菜碱与植物抗逆性关系的研究进展[J].农业科学研究,2006,27(3):66-68.