海南莺歌海近岸陆地土壤及潮间带沉积物可培养真菌资源分析
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摘要
从莺歌海近岸土壤及潮间带沉积物中分离到可培养真菌,通过ITS序列比对,分析菌株的相似性.结果表明:16株分离菌株菌落形态为丝状或黏稠状; Blast比对显示,它们分别属于子囊菌门及担子菌门,其中,8株菌株(YGHWSF5,YGHWSF7,YGHLSF6,YGHLSF2,YGHLSF5,YGHLSF8,YGHLSF3,YGHLSF7)与Penicillium及Aspergillus相似性最高;而菌株YGHLSF1,YGHLSF4,YGHLSF9,YGHWSF1和YGHWSF2,YGHWSF8,YGHWSF10,YGHWSF11分别与Pseudozyma、Cladosporium、Acremonium、Moesziomyces、Parengyodontium、Graphium、Talaromyces相似性最高.系统发育学分析显示与Blast比对结果相似,16株菌株分别聚为8个分支,展现了莺歌海海岸带区域存在的可培养真菌资源多样性显著,可进一步收集资源,为进一步海洋功能菌开发提供素材.
In this study,the similarity of culturable fungi,which were isolated from the soil and intertidal sediments of the Yinggehai Hainan,was analyzed by ITS sequence alignment. The results showed that the colony morphology of the 16 isolates is filamentous or viscous. According to Blast alignment,the 16 isolates belong to Ascomycetes and Basidiomycetes,respectively,among which 8 strains—YGHWSF5,YGHWSF7,YGHLSF6,YGHLSF2,YGHLSF5,YGHLSF8,YGHLSF3 and YGHLSF7—have the highest similarity with Penicillium and Aspergillus while YGHLSF1 has the highest similarity with Pseudozyma,YGHLSF4 with Cladosporium,YGHLSF9 with Acremonium,YGHWSF1 and YGHWSF2 have the highest similarity with Moesziomyces,YGHWSF8 has the highest similarity with Parengyodontium,YGHWSF10 with Graphium,and YGHWSF11 with Talaromyces. Compared with Blast alignment,Phylogenetic analysis showed similar results—that the 16 strains are clustered into 8 clades—that in the coastal zone of Yinggehai,there is a significant diversity of culturable fungi resources which can be collected as a material basis for the further development of marine functional fungi.
In this study,the similarity of culturable fungi,which were isolated from the soil and intertidal sediments of the Yinggehai Hainan,was analyzed by ITS sequence alignment. The results showed that the colony morphology of the 16 isolates is filamentous or viscous. According to Blast alignment,the 16 isolates belong to Ascomycetes and Basidiomycetes,respectively,among which 8 strains—YGHWSF5,YGHWSF7,YGHLSF6,YGHLSF2,YGHLSF5,YGHLSF8,YGHLSF3 and YGHLSF7—have the highest similarity with Penicillium and Aspergillus while YGHLSF1 has the highest similarity with Pseudozyma,YGHLSF4 with Cladosporium,YGHLSF9 with Acremonium,YGHWSF1 and YGHWSF2 have the highest similarity with Moesziomyces,YGHWSF8 has the highest similarity with Parengyodontium,YGHWSF10 with Graphium,and YGHWSF11 with Talaromyces. Compared with Blast alignment,Phylogenetic analysis showed similar results—that the 16 strains are clustered into 8 clades—that in the coastal zone of Yinggehai,there is a significant diversity of culturable fungi resources which can be collected as a material basis for the further development of marine functional fungi.
引文
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[8]郑贺云，黎志坤，李超，等．新疆阿克苏地区盐碱地细菌类群多样性及优势菌群分析[J]．微生物学通报，2012,39(7):1031-1043.
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[10]姜芳燕，杨宁，冯慧敏，等．不同活性物质对大海马烂尾病病原菌的体外抑菌作用研究[J]．海南热带海洋学院学报，2017,24(5):14-20.
[2]金静，李宝笃．海洋真菌多样性的研究进展[J]．海洋湖沼通报，2004(2):90-94.
[3]CHENY G,JI Y L,YU HS,et al. A new Neotyphodium species from Festuca parvigluma Steud. grown in China[J]. Mycologia,2009,101(5):681-685.
[4]THOMPSON J D,HIGGINS D G,GIBSON T J. Clustal W:improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice[J]. Nucleic Acids Research,1994,22:4673-4680.
[5]TAMURA K,PETERSON D,PETERSON N,et al. MEGA5:molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J]. Molecular Biology and Evolution,2011,28:2731-2739.
[6]刘玉，温翠屏，李艳萍，等．红树植物对根域真菌生态的影响[J]．生态学报，2015(8):2473-2480.
[7]杨丹丹，黎乾，黄晶晶，等．岱山盐场可培养嗜盐菌的多样性及其产酶活性筛选[J]．应用生态学报，2012,23(11):3103-3108.
[8]郑贺云，黎志坤，李超，等．新疆阿克苏地区盐碱地细菌类群多样性及优势菌群分析[J]．微生物学通报，2012,39(7):1031-1043.
[9]肖炜，杨亚玲，刘宏伟，等．昆明盐矿古老岩盐沉积中可培养细菌多样性研究[J]．微生物学报，2006,46(6):967-972.
[10]姜芳燕，杨宁，冯慧敏，等．不同活性物质对大海马烂尾病病原菌的体外抑菌作用研究[J]．海南热带海洋学院学报，2017,24(5):14-20.