电纺SF/bFGF纳米纤维膜用于皮肤缺损修复
|
摘要
本文采用静电纺丝制备含有碱性成纤维细胞生长因子(Basic fibroblast growth factor,bFGF)和丝素蛋白(Silk fibroin,SF)的纳米纤维膜(SF/bFGF)。利用扫描电镜研究SF/bFGF复合膜片的内部形态和结构,通过小鼠成纤维细胞L929迁移实验和小鼠皮肤缺损修复实验分析SF/bFGF的体内外活性。结果表明,SF/bFGF能促进成纤维细胞迁移且SF/bFGF纳米纤维膜对小鼠皮肤缺损有促进修复作用。
The nanofiber membrane(SF/bFGF) containing basic fibroblast growth factor(bFGF) and silk fibroin(SF)was prepared by electrostatic spinning. The internal morphology and structure of SF/bFGF were studied by scanning electron microscopy. In addition, the bioactivity of SF/bFGF in vitro and in vivo were detected by the migration of mouse fibroblasts L929 and mouse skin defect repair experiment. The results indicated that SF/bFGF could promote the migration of fibroblasts and played a role in the repair of mouse skin defects.
The nanofiber membrane(SF/bFGF) containing basic fibroblast growth factor(bFGF) and silk fibroin(SF)was prepared by electrostatic spinning. The internal morphology and structure of SF/bFGF were studied by scanning electron microscopy. In addition, the bioactivity of SF/bFGF in vitro and in vivo were detected by the migration of mouse fibroblasts L929 and mouse skin defect repair experiment. The results indicated that SF/bFGF could promote the migration of fibroblasts and played a role in the repair of mouse skin defects.
引文
[1]Foss Cristina,Merzari Enrico,Migliaresi Claudio,et al.Silk Fibroin/Hyaluronic Acid 3D Matrices for Cartilage Tissue Engineering[J].Biomacromolecules,2013,14(1):38-47.
[2]Tao Li,Xiongbo Song,Changmei Weng,et al.Enzymatically Crosslinked and Mechanically Tunable Silk Fibroin/Pullulan Hydrogels for Mesenchymal Stem Cells Deliver[J].International Journal of Biological Macromolecules,2018,115(10):300-307.
[3]Shuichi Ogino,Naoki Morimoto,Michiharu Sakamoto,et al.Efficacy of the Dual Controlled Release of HGF and bFGFImpregnated with a Collagen/Gelatin Scaffold[J].Journal of Surgical Research,2018,221:173-182.
[4]Michiharu Sakamoto,Naoki Morimoto,Shuichi Ogino,et al.Efficacy of Gelatin Gel Sheets in Sustaining the Release of Basic Fibroblast Growth Factor for Murine Skin Defects[J].Journal of Surgical Research,2015,22(8):1-20.
[5]Yang Lei,Zheng Zhaozhu,Qian Cheng,et al.Curcumin-Functionalized Silk Biomaterials for Anti-Aging Utility[J].Journal of colloid and interface science,2017,496(10):66-77.
[2]Tao Li,Xiongbo Song,Changmei Weng,et al.Enzymatically Crosslinked and Mechanically Tunable Silk Fibroin/Pullulan Hydrogels for Mesenchymal Stem Cells Deliver[J].International Journal of Biological Macromolecules,2018,115(10):300-307.
[3]Shuichi Ogino,Naoki Morimoto,Michiharu Sakamoto,et al.Efficacy of the Dual Controlled Release of HGF and bFGFImpregnated with a Collagen/Gelatin Scaffold[J].Journal of Surgical Research,2018,221:173-182.
[4]Michiharu Sakamoto,Naoki Morimoto,Shuichi Ogino,et al.Efficacy of Gelatin Gel Sheets in Sustaining the Release of Basic Fibroblast Growth Factor for Murine Skin Defects[J].Journal of Surgical Research,2015,22(8):1-20.
[5]Yang Lei,Zheng Zhaozhu,Qian Cheng,et al.Curcumin-Functionalized Silk Biomaterials for Anti-Aging Utility[J].Journal of colloid and interface science,2017,496(10):66-77.