The lacustrine stromatolites from the Oligocene Yaxicuo Formation of the northern Tibetan Plateau display regular growth rhythms of dark (organic-rich layer) and light (sediment-rich layer) lamination alternations with millimeter scale. The analysis results showed that the dark lamina in stromatolites commonly has relatively higher δ18O and δ13C values than the light lamina. The authors suggest that the couplets of laminae in lacustrine stromatolites indicate the origin of annual lamination. This study demonstrates the lamination in lacustrine stromatolites can provide a kind of geologic material with high-resolution to reconstruct terrestrial palaeoclimate and palaeoenvironment changes during the geologic history.