More than 70 witherite and barite deposits (or points) are located in Southern Qinling Mountains, which is a unique large Ba metallogenic belt in the world. By microthermometry analysis, the peak of fluid inclusions homogeneous temperature has a increasing trend from barite to witherite. The δ34S values of barite of the deposits are higher than those of the same period water of ocean basin (except one δ34S value of barite of Wenyu deposit, which is close to those of the same period water of ocean basin), which shows that the 34S enrichment in barite could be due to the reduction from germs to sulfate. Witherite deposits of