Seep carbonates collected from Green Canyon block 140, Gulf of Mexico, could be divided into three types: nodule-rich carbonates, bioclast-rich carbonates and massive carbonates. XRD investigation shows that high Mg-calcite and aragonite are the dominate minerals. Additionally, nodule-rich carbonates contain minor amounts of dolomite and bioclast-rich carbonates include some low Mg-calcite. Peloids, clotted microfabric and pyrite framboids are developed in carbonates and suggest a genesis linked with bacterial activities. The carbonates exhibit a large variation in ^δ13 values (-36.1‰ to 5.1‰), suggesting multiple carbon sources that include thermogenic methane, seawater and residual CO2 from methanogenesis. Shells cemented in nodule-rich carbonates show ^14C ages between 46.5 ka and 25.8 ka BR bioclast-rich carbonates formed from 17.6 ka to 11.7 ka BE and massive carbonates have ^14C ages of 1.2 ka BR suggesting that seep activity was discontinuous.