浙江汉族人群21个非CODIS系统STR基因座的遗传多态性调查
|
摘要
目的调查21个非CODIS系统STR在浙江汉族人群的遗传多态性,为其法医学应用提供基础数据。方法应用AGCU21+1荧光标记复合扩增系统,对浙江汉族481名无关个体进行21个STR基因座的复合扩增,用ABI3130XL型基因分析仪对扩增产物进行检测,并统计其STR基因座的群体遗传学参数。结果获得21个STR基因座的等位基因频率分布,分别检出8、11、9、10、8、9、6、5、13、9、6、15、8、7、8、9、8、9、9、16、7个等位基因,并分别获得21个STR基因座的H、He、DP、PM及EP等法医遗传学参数。结论21个STR基因座具有较强个体识别能力,可应用于法庭科学中的个体识别与亲权鉴定。
Objective To Study the genetic polymorphism of short tandem repeat(STR) loci of Han population in Zhejiang province,and evaluate their forensic application.Method D6S474、D12ATA63、D22S1045、D10S1248、D1S1677、D11S4463、D1S1627、D3S4529、D2S441、D6S1017、D4S2408、D19S433、D17S1301、D1GATA113、D18S853、D20S482、D14S1434、D9S1122、D2S1776、D10S1435 and D5S2500 loci were amplified by using an AGCU 21+1 PCR amplification Kit,and analyzed by 3130XL genetic analyzer.481 unrelated individuals of Han population in Zhejiang province were investigated to determine the distributions of allele frequencies.Results Allele distributions of the 21 loci have been obtained,8、11、9、10、8、9、6、5、13、9、6、15、8、7、8、9、8、9、9、16、7 alleles were found in the preview 21 STR loci.The Combined discrimination power(DP)and excluding probability of paternity(EPP)of 21 loci is 1-2.26×10(-20)and 0.999999518,respectively.Conclusion The 21 STR loci are highly polymorphic and suitable for forensic personal identification and paternity testing.
Objective To Study the genetic polymorphism of short tandem repeat(STR) loci of Han population in Zhejiang province,and evaluate their forensic application.Method D6S474、D12ATA63、D22S1045、D10S1248、D1S1677、D11S4463、D1S1627、D3S4529、D2S441、D6S1017、D4S2408、D19S433、D17S1301、D1GATA113、D18S853、D20S482、D14S1434、D9S1122、D2S1776、D10S1435 and D5S2500 loci were amplified by using an AGCU 21+1 PCR amplification Kit,and analyzed by 3130XL genetic analyzer.481 unrelated individuals of Han population in Zhejiang province were investigated to determine the distributions of allele frequencies.Results Allele distributions of the 21 loci have been obtained,8、11、9、10、8、9、6、5、13、9、6、15、8、7、8、9、8、9、9、16、7 alleles were found in the preview 21 STR loci.The Combined discrimination power(DP)and excluding probability of paternity(EPP)of 21 loci is 1-2.26×10(-20)and 0.999999518,respectively.Conclusion The 21 STR loci are highly polymorphic and suitable for forensic personal identification and paternity testing.
引文
[1]吴微微,徐林苗,朱虹辉,等.浙江汉族人群9个STR基因座的遗传多态性调查[J].中国法医学杂志,2001,16(1):38-40.
[2]郝宏蕾,吴微微,潘立鹏,等.浙江汉族人群15个STR基因座遗传多态性[J].中国法医学杂志,2006,21(5):303-304.
[3]徐振波,杜舟,王传海,等.DNA数据库大样本量检验的自动化应用[J].中国法医学杂志,2006,21(4):231-232.
[4]Peakall,R.,Smouse,P.E.,GENALEX 6:genetic a-nalysis in Excel.Population genetic software for teach-ing and research[J].Molecular Ecology Notes,2006(6):288-295.
[5]Gill P.A new method of STRinterpretation using infer-ential logic-development of a crininal intelligence data-base[J].Int J Leg Med,1996,109(1):14-22.
[2]郝宏蕾,吴微微,潘立鹏,等.浙江汉族人群15个STR基因座遗传多态性[J].中国法医学杂志,2006,21(5):303-304.
[3]徐振波,杜舟,王传海,等.DNA数据库大样本量检验的自动化应用[J].中国法医学杂志,2006,21(4):231-232.
[4]Peakall,R.,Smouse,P.E.,GENALEX 6:genetic a-nalysis in Excel.Population genetic software for teach-ing and research[J].Molecular Ecology Notes,2006(6):288-295.
[5]Gill P.A new method of STRinterpretation using infer-ential logic-development of a crininal intelligence data-base[J].Int J Leg Med,1996,109(1):14-22.
目录