四川汉族人群15个短串联重复序列基因座遗传多态性分析
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摘要
目的获得15个短串联重复(short tandem repeat,STR)基因座在四川汉族人群中的群体遗传学数据。方法654份血样采自四川地区无血缘关系的汉族个体。Chelex法提取DNA,PCR复合扩增,自动基因分析仪电泳,收集电泳结果数据,基因扫描分析软件计算扩增产物片段相对大小,基因分型软件进行样本基因型分型。结果全部样本的每个STR基因座都获得了清晰的基因型分型结果。15个STR基因座的基因型分布符合Hardy-Weinberg平衡。15个STR基因座的杂合度介于0.6101—0.8654之间。累计非父排除率和累计个人识别率为0.999998766和>0.999999999。结论经1次扩增电泳可获得15个STR基因座的基因型分型结果,累计非父排除率和累计个人识别率较高,适合作为四川汉族人群的遗传标记,用于人类学、疾病连锁分析、法医学亲权鉴定和个体识别等领域的研究。
Objective To acquire the population genetic data of fifteen short tandem repeat (STR) loci in Sichuan Han population.Methods A total of 654 blood specimens were collected from the unrelated individuals in Sichuan Han population. DNA was extracted with Chelex method and amplified by multiplex PCR technique. The PCR products were analyzed by an automatic genetic analyzer; the relative fragment lengths of PCR products were calculated by genescan analysis software and afterward genotyped by genotype software.Results Fifteen STR loci of each sample showed a successful result of genotyping. The gene frequency of these 15 STR loci was in Hardy-Weinberg equilibrium. The heterozygosities of the fifteen STR loci in Sichuan Han population were found to be 0.6101—0.8654; the combined exclusion probability and discrimination power for the 15 STR loci in Sichuan Han population were determined to be 0.999998766 and above 0.999999999,respectively.Conclusion The distinct genotype of 15 STR loci could be unveiled just through PCR and electrophoresis once,and a higher measured value could be obtained for both the combined discrimination power and the combined exclusion probability; the fifteen STR loci can be used as genetic markers of Sichuan Han population in anthropological studies,linkage analysis of genetic diseases,parentage testing and personal identification in forensic medicine.
Objective To acquire the population genetic data of fifteen short tandem repeat (STR) loci in Sichuan Han population.Methods A total of 654 blood specimens were collected from the unrelated individuals in Sichuan Han population. DNA was extracted with Chelex method and amplified by multiplex PCR technique. The PCR products were analyzed by an automatic genetic analyzer; the relative fragment lengths of PCR products were calculated by genescan analysis software and afterward genotyped by genotype software.Results Fifteen STR loci of each sample showed a successful result of genotyping. The gene frequency of these 15 STR loci was in Hardy-Weinberg equilibrium. The heterozygosities of the fifteen STR loci in Sichuan Han population were found to be 0.6101—0.8654; the combined exclusion probability and discrimination power for the 15 STR loci in Sichuan Han population were determined to be 0.999998766 and above 0.999999999,respectively.Conclusion The distinct genotype of 15 STR loci could be unveiled just through PCR and electrophoresis once,and a higher measured value could be obtained for both the combined discrimination power and the combined exclusion probability; the fifteen STR loci can be used as genetic markers of Sichuan Han population in anthropological studies,linkage analysis of genetic diseases,parentage testing and personal identification in forensic medicine.
引文
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[6]王振原,马骏,徐永城,等.西安地区汉族人群13个STR基因座多态性分析.中南大学学报(医学版),2005,30(2):236—240
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[8]郝宏蕾,吴微微,潘立鹏,等.浙江汉族人群15个STR基因座遗传多态性.中国法医学杂志,2006,21(5):303—304
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[10]李勇,江华,刘杰,等.黑龙江蒙古族群体15个STR基因座多态性.中国法医学杂志,2009,24(4):276—277
[11]Kim YL,Hwang JY,Kim YJ,et al.Allele frequencies of15STR loci using AmpF/STR Identifiler kit in a Korean population.Forensic Sci Int,2003,136(1—3):92—95
[12]Barni F,Berti A,Pianese A,et al.Allele frequencies of15au-tosomal STR loci in the Iraq population with comparisons to other populations from the middle-eastern region.Forensic Sci Int,2007,167(1):87—92
[13]Szczerkowska Z,Kapin′ska E,Wysocka J,et al.Northern Pol-ish population data and forensic usefulness of15autosomal STR loci.Forensic Sci Int,2004,144(1):69—71
[14]Hernández-Gutirrez S,Hernández-Franco P,Martínez-Tripp S,et al.STR data for15loci in a population sample fromthe central region of Mexico.Forensic Sci Int,2005,151(1):97—100
[15]Beleza S,Alves C,Reis F,et al.17STR data(AmpF/STR Identifiler and Powerplex16System)from Cabinda(Angola).Forensic Sci Int,2004,141(2—3):193—196
[16]李宁,苏玉虹,席焕久,等.西藏藏族人群15个短串联重复序列基因座的遗传多态.遗传,2008,30(7):851—856
[2]Walsh PS,Metzger DA,Higuchi R.Chelex100as a medium for simple extraction of DNA for PCR-based typing from forensic ma-terial.Biotechniques,1991,10(4):506—513
[3]侯一平.法医DNA分析的科学证据意义.中国法医学杂志,2001,16(2):118—120,126
[4]李英碧,吴谨,侯一平,等.成都汉族群体15个短串联重复序列基因座遗传多态性.中华医学遗传学杂志,2005,22(2):169—173
[5]张贵芹,裴黎,马原,等.内蒙古汉族群体15个STR基因座遗传多态性.中国法医学杂志,2008,23(4):265—266
[6]王振原,马骏,徐永城,等.西安地区汉族人群13个STR基因座多态性分析.中南大学学报(医学版),2005,30(2):236—240
[7]刘长晖,刘超,杨电,等.广州地区人群15个STR基因座遗传多态性.中国法医学杂志,2007,22(5):327—329
[8]郝宏蕾,吴微微,潘立鹏,等.浙江汉族人群15个STR基因座遗传多态性.中国法医学杂志,2006,21(5):303—304
[9]王剑,张伟,黄玉立,等.中国鄂伦春族人群15个STR基因座多态性研究.刑事技术,2004,5:13—15
[10]李勇,江华,刘杰,等.黑龙江蒙古族群体15个STR基因座多态性.中国法医学杂志,2009,24(4):276—277
[11]Kim YL,Hwang JY,Kim YJ,et al.Allele frequencies of15STR loci using AmpF/STR Identifiler kit in a Korean population.Forensic Sci Int,2003,136(1—3):92—95
[12]Barni F,Berti A,Pianese A,et al.Allele frequencies of15au-tosomal STR loci in the Iraq population with comparisons to other populations from the middle-eastern region.Forensic Sci Int,2007,167(1):87—92
[13]Szczerkowska Z,Kapin′ska E,Wysocka J,et al.Northern Pol-ish population data and forensic usefulness of15autosomal STR loci.Forensic Sci Int,2004,144(1):69—71
[14]Hernández-Gutirrez S,Hernández-Franco P,Martínez-Tripp S,et al.STR data for15loci in a population sample fromthe central region of Mexico.Forensic Sci Int,2005,151(1):97—100
[15]Beleza S,Alves C,Reis F,et al.17STR data(AmpF/STR Identifiler and Powerplex16System)from Cabinda(Angola).Forensic Sci Int,2004,141(2—3):193—196
[16]李宁,苏玉虹,席焕久,等.西藏藏族人群15个短串联重复序列基因座的遗传多态.遗传,2008,30(7):851—856
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