Development of an electrochemical flow injection immunoassay (FIIA) for the real-time monitoring of biospecific interactions

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• 作者：Killard ; Anthony J. ; Zhang ; Shanqing ; Zhao ; Huijun ; John ; Richard ; Iwuoha ; Emmanuel I. ; Smyth ; Malcolm R.
• 关键词：Flow injection ; Immunoassay ; Biosensor ; Screen printed electrode ; Polyaniline
• 刊名：Analytica Chimica Acta
• 出版年：1999
• 出版时间：November 22, 1999
• 年：1999
• 卷：400
• 期：1-3
• 页码：109-119
• 全文大小：267 K

文摘

Not only are sensors a revolution in analysis; they themselves are also experiencing a revolution brought about by parallel developments in sensor fabrication techniques and materials, polymer chemistry, signal processing methodologies, the increased use of biomolecular processes as a means of analyte detection, and the coupling of sensors to other techniques such as flow injection analysis. Many of these developments have been incorporated into the present study, which we are undertaking in the development of our immunosensor technology. The system described here utilises screen-printed electrodes which are low-cost, disposable devices that are simple to fabricate. Incorporated into our sensor is the electroactive polymer, polyaniline, which brings about mediatorless redox coupling between the electrode and biomolecular components attached to the polymer surface. This system also utilises enzyme-labelled antibodies as the biomolecular recognition component for the analysis of the test analyte, biotin. The system has also been integrated into a flow injection system. This has led to the monitoring of real-time antibody-antigen interactions using electrochemical methods and foreshadows the development of single-step immunosensors. Publisher: Elsevier Science Language of Publication: English Item Identifier: S0003-2670(99)00611-X Publication Type: Article ISSN: 0003-2670 Cited by:

1. Pemberton, R.M.; Hart, J.P.; Mottram, T.T.,""An electrochemical immunosensor for milk progesterone using a continuous flow system¹""Biosensors and Bioelectronics2001pp. 715-723
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2. Zhang, Chengxiao; Gao, Qiang; Aizawa, Masuo,""Flow injection analytical system for glucose with screen-printed enzyme biosensor incorporating Os-complex mediator""Analytica Chimica Acta2001pp. 33-41
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3. Morrin, Aoife; Guzman, Alicia; Killard, Anthony J.; Pingarron, Jose M.; Smyth, Malcolm R.,""Characterisation of horseradish peroxidase immobilisation on an electrochemical biosensor by colorimetric and amperometric techniques""Biosensors and Bioelectronics2003pp. 715-720
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4. Gao, Qiang; Ma, Ying; Cheng, Zhiliang; Wang, Weidong; Yang, Xiurong,""Flow injection electrochemical enzyme immunoassay based on the use of an immunoelectrode strip integrate immunosorbent layer and a screen-printed carbon electrode""Analytica Chimica Acta2003pp. 61-70
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5. Grennan, Kathleen; Strachan, Gillian; Porter, Andrew J.; Killard, Anthony J.; Smyth, Malcolm R.,""Atrazine analysis using an amperometric immunosensor based on single-chain antibody fragments and regeneration-free multi-calibrant measurement""Analytica Chimica Acta2003pp. 287-298
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