Rabies vaccination in dogs using a dissolving microneedle patch

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• 作者：Jaya M. Arya^a ; Kristopher Dewitt^b ; Maya Scott-Garrard^b ; Yu-Wei Chiang^b ; Mark R. Prausnitz^a ; ^{prausnitz@gatech.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Microneedle patch ; Transdermal drug delivery ; Intradermal skin vaccination ; Rabies vaccine ; DNA vaccine ; Veterinary vaccination of dogs
• 刊名：Journal of Controlled Release
• 出版年：2016
• 出版时间：10 October 2016
• 年：2016
• 卷：239
• 期：Complete
• 页码：19-26
• 全文大小：935 K

文摘

Because humans get rabies primarily through dog bites, stray dog population control and mass or mandatory vaccination of domestic dogs and other animals has virtually eliminated human rabies in industrialized countries. However, thousands of people in developing countries die of rabies each year due to the inability to control dog populations and implement mass vaccination because of financial, logistical and other challenges. The availability of an easier-to-administer and more cost-effective vaccine may help to address some of these issues. Here, we propose the use of dissolving microneedle patches for simple and potentially cost-effective rabies vaccination, and assess the safety and immunogenicity of microneedle patch vaccination using a rabies DNA vaccine in dogs. The vaccine was stable upon formulation and storage for at least 3 weeks at 4 °C in a microneedle patch. For vaccination, the patches were applied to the inner ear by hand without an applicator. Microneedle patches were well tolerated in the skin, with mild erythema, minimal wheal formation and complete resolution of skin reactions within 7 days, and generated no systemic adverse events. Microneedle patches were at least as immunogenic as intramuscular injection at the same dose, as demonstrated by similar serum neutralizing antibody titers. A ten-fold lower vaccine dose administered by microneedle patch generated a weaker immune response compared to full-dose intramuscular vaccination. We conclude that dissolving microneedle patches may provide an innovative approach to mass vaccination of dogs.