Evaluation of Spoligotyping and MIRU-VNTR for Mycobacterium bovis in Xinjiang, China

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• 作者：Zhaogang Sun^a ; ¹ ; Rui Cao^b ; ¹ ; Miao Tian^a ; Xuxia Zhang^a ; Xiyue Zhang^a ; Yan Li^a ; Yuhui Xu^d ; Weixing Fan^c ; Baoxu Huang^c ; Chuanyou Li^a ; ^{sunzg75@163.com} ; ^{lichuanyou6688@hotmail.com}
• 关键词：M. bovis ; Cow ; Isolation ; Spoligotyping ; MIRU-VNTR ; Epidemic
• 刊名：Research in Veterinary Science
• 出版年：2012
• 出版时间：April 2012
• 年：2012
• 卷：92
• 期：2
• 页码：236-239
• 全文大小：163 K

文摘

Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB) in cattle, is also a pathogen for human and other mammals. In this study, 406 cows were screened for bTB by both single intradermal comparative cervical tuberculin (SICCT) test and IFN-¦Ã assay. 135 M. bovis were isolated from 31 SICCT and IFN-¦Ã double-positive cows in Xinjiang Uygur Autonomous Region of China. Spoligotyping and MIRU-VNTR were evaluated for genotyping, and 4 and 7 genotypes were identified, respectively. A new combination of nine MIRU-VNTR loci was most discriminative for M. bovis clones from Xinjiang. Interestingly, two new spoligotypes (SB1903 and SB1904) and special repeat numbers of three loci (ETR-D, QUB 1895 and QUB 3336) were discovered in this study. These results indicated a specific epidemic conservation in Xinjiang, China. M. bovis strains with the unique genotypes were isolated from the herds maintaining parent cows imported from the bTB-free countries, suggesting a possible transmission from the local breed of Xinjiang brown cattle.