Evaluation of infection status in Chinese swine with porcine reproductive and respiratory syndrome virus by nested RT-PCR targeting nsp2 gene
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- 作者：Minxia Zhanga ; 474659627@qq.com" class="auth_mail" title="E-mail the corresponding author ; Xinan Lia ; xinan.1987@163.com" class="auth_mail" title="E-mail the corresponding author ; Xinna Caia ; caixinna@126.com" class="auth_mail" title="E-mail the corresponding author ; Yajin Qua ; huiyaqui@163.com" class="auth_mail" title="E-mail the corresponding author ; Dongfang Hua ; dongfang_hu@sina.com" class="auth_mail" title="E-mail the corresponding author ; Lin Lva ; lvlin0911@163.com" class="auth_mail" title="E-mail the corresponding author ; Sidang Liua ; liusid@sdau.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Xiaomin Zhaob ; xmzhao66@sdau.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Leyi Wangc ; wang.1074@buckeyemail.osu.edu" class="auth_mail" title="E-mail the corresponding author ; Yihong Xiaoa ; xiaoyihong01@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Porcine reproductive and respiratory syndrome virus ; Nested RT-PCR ; nsp2 ; Deletion ; Co-infection
- 刊名：Infection, Genetics and Evolution
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：44
- 期：Complete
- 页码：55-60
- 全文大小：542 K
文摘
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A nested RT-PCR, targeting nsp2 gene, to differentiateing PRRSV variants was developed.
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192 of 550 clinical samples from non-PRRSV-vaccinated pig herd were tested positively by the nested RT-PCR.
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Of the 192 positive samples, 107 were infected with a single strain and 85 were infected with two strains.
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HP-PRRSV containing a 90-bp deletion in the nsp2 gene remained the predominant strain.
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This assay could be useful in the area where different type of live vaccines were used.