Insights from native mass spectrometry and ion mobility-mass spectrometry for antibody and antibody-based product characterization
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- 作者：Guillaume Terrala ; b ; Alain Beckc ; Sarah Cianfé ; rania ; b ; sarah.cianferani@unistra.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Native mass spectrometry ; Ion mobility mass spectrometry ; Monoclonal antibody ; Antibody-drug-conjugate ; Bispecific antibody ; Antibody/antigen complex
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：1 October 2016
- 年：2016
- 卷：1032
- 期：Complete
- 页码：79-90
- 全文大小：3054 K
文摘
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Native MS allows rapid intact mAb analysis with limited sample handling.
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High resolution native MS is required to distinguish species with similar masses.
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IM-MS reveals mAb conformations.
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Drug load profiles and average DARs of ADCs can be assessed by native MS and IM-MS.
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The formation of bispecific mAbs and mAbs/antigen complexes can be monitored by native MS and IM-MS.