Taxus metabolomics: methyl jasmonate preferentially induces production of taxoids oxygenated at C-13 in Taxus x media cell cultures
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- 作者：Ketchum ; Raymond E.B. ; Rithner ; Christopher D. ; Qiu ; Deyou ; Kim ; You Sun ; Williams ; Robert M. ; et. al.
- 关键词：Taxus x media cv. Hicksii ; Taxus cuspidata ; Taxaceae ; Yew ; Metabolomics ; Metabolic profiling ; Taxane diterpenoids ; Taxoids ; Paclitaxel ; Taxol®
- 刊名：Phytochemistry
- 出版年：2003
- 出版时间：March, 2003
- 年：2003
- 卷：62
- 期：6
- 页码：901-909
- 全文大小：220 K
文摘
Cells from suspension cultures of Taxus cuspidata were extracted with pentane as a source of relatively non-polar taxoids. Of the 13 taxoids identified in this fraction, eight were oxygenated at C-14 and two had not been previously described. These taxoids, along with existing taxoid standards, were employed to profile the metabolites of Taxus x media cv. Hicksii cell suspension cultures induced with methyl jasmonate to produce paclitaxel (Taxol®). The majority of the taxoid metabolites produced in these induced cultures were oxygenated at C-13, and not C-14.