Engineering of an Lrp family regulator SACE_Lrp improves erythromycin production in Saccharopolyspora erythraea

详细信息    查看全文

• 作者：Jing Liu^a ; Yunfu Chen^a ; Weiwei Wang^a ; Min Ren^a ; Panpan Wu^a ; Yansheng Wang^a ; Changrun Li^a ; Lixin Zhang^a ; ^b ; Hang Wu^a ; ^{wuh2007@gmail.com} ; David T. Weaver^a ; ^{David.weaver.t@gmail.com} ; Buchang Zhang^a ; ^{zhbc@ahu.edu.cn}
• 关键词：Saccharopolyspora erythraea ; Leucine-responsive regulatory protein ; Branched-chain amino acid ; Erythromycin ; Engineering
• 刊名：Metabolic Engineering
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：39
• 期：Complete
• 页码：29-37
• 全文大小：885 K
• 卷排序：39

文摘

SACE_Lrp negatively regulates erythromycin production in Saccharopolyspora erythraea. SACE_Lrp directly regulates the BCAA transport and indirectly regulates the BCAA catabolism in S. erythraea. SACE_Lrp is the first reported Lrp showing two-directional response to its amino acid-effectors. A high erythromycin-producing strain has been constructed by engineering the SACE_Lrp regulatory elements.