Occurrence of qualitative and quantitative polymorphism at donkey beta-Lactoglobulin II locus

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• 作者：Lina Chianese ; Carmela De Simone ; Pasquale Ferranti ; Rosalba Mauriello ; Angela Costanzo ; Maria Quarto ; Giuseppina Garro ; Gianluca Picariello ; Gianfranco Mamone ; Luigi Ramunno
• 关键词：Donkey milk whey proteins ; Proteomics ; Genetic polymorphism ; Beta-lactoglobulin
• 刊名：Food Research International
• 出版年：November, 2013
• 年：2013
• 卷：54
• 期：1
• 页码：1273-1279
• 全文大小：754 K

文摘

With the use of a proteomic approach a genetic screening of 70 individual donkey milk samples, from two autochthonous breeds reared in Italy, was carried out to determine the genetic polymorphism at donkey 尾-Lgs loci. The achieved results showed the occurrence of the new 尾-Lg II E genetic variant, differing from 尾-Lg II D reference for D Asp²/E Asn², D Arg¹⁸/E Lys¹⁸ and D Val²⁵/E Ala²⁵ amino acid substitutions with a M_r 18 256 Da. Moreover a quantitative polymorphism seemed to affect the donkey 尾-Lg II locus since four phenotypes, with a relative quantitative percentage respect to total whey protein ranging from 0% to 3.65%, were detected by HPLC analysis.