Novel assays of multiple lymphocyte functions in whole blood measure: New mechanisms of action of mycophenolate mofetil in vivo

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• 作者：Barten ; Markus J. ; van Gelder ; Teun ; Gummert ; Jan F. ; Shorthouse ; Randi ; Morris ; Randall E.
• 关键词：Whole blood ; Lymphocyte functions ; Mycophenolate mofetil ; Mechanisms of action
• 刊名：Transplant Immunology
• 出版年：2002
• 出版时间：June, 2002
• 年：2002
• 卷：10
• 期：1
• 页码：1-14
• 全文大小：660 K

文摘

The antiproliferative effects of MMF are believed to be the mechanism of its immunosuppressive action. We further investigated the mechanisms of action by assessing the pharmacodynamics (PD) of MMF in treated animals using whole blood assays not only of lymphocyte proliferation but also of activation. In vitro, different MPA concentrations were added to rat whole blood. In vivo, Lewis rats were treated with single doses of 5, 10 or 20 mg/kg MMF (n