Pyrrolizidine alkaloid clivorine inhibits human normal liver L-02 cells growth and activates p38 mitogen-activated protein kinase in L-02 cells

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• 作者：Ji ; Li-Li ; Zhao ; Xian-Guo ; Chen ; Li ; Zhang ; Mian ; Wang ; Zheng-Tao
• 关键词：Clivorine ; Pyrrolizidine alkaloid ; L-02 cell ; p38 Mitogen-activated protein kinase ; PAs ; pyrrolizidine alkaloids ; MAP Kinase ; mitogen-activated protein Kinase ; ERK1/2 ; extracellular signal-related kinases ; RPMI 1640 ; Roswell Park Memorial Institute 1640
• 刊名：Toxicon
• 出版年：2002
• 出版时间：December, 2002
• 年：2002
• 卷：40
• 期：1
• 页码：1685-1690
• 全文大小：337 K

文摘

Clivorine, a pyrrolizidine alkaloid extracted from Chinese medicinal plant Ligularia hodgsonii Hook significantly inhibited human normal liver L-02 cells proliferation and decreased L-02 cells viability. The results of western blot showed that clivorine strongly evoked phosphorylation of p38 mitogen-activated protein (MAP) Kinase in L-02 cells, but had no effect on extracellular signal-related kinases MAP Kinase phosphorylation. Moreover, another pyrrolizidine alkaloid monocrotaline had no effect on phosphorylation of p38 MAP Kinase in L-02 cells. These studies document the effects of pyrrolizidine alkaloid clivorine on the MAPK cascade and on the growth of human normal liver L-02 cells for the first time, which may be a possible reason for the toxic effects observed in those plants containing pyrrolizidine alkaloids.