The present 87Sr/86Sr isotopic study of the alluvial aquifer in the southern part of the Upper Rhine valley was undertaken in the framework of a multidisciplinary hydrogeochemical research project searching for cost-effective geochemical methods in hydrogeothermal energy exploration. The aim is to identify possible imprints of deep fluids in shallow aquifers using 87Sr/86Sr ratios as tracer. Strontium ratios and Sr contents of Quaternary groundwater (N=17) range from 0.70852 to 0.71005 and 0.28 to 0.92 mg/l, respectively. Groundwater from a Tertiary deep aquifer has an unexpected low 87Sr/86Sr ratio of 0.70855 and a high Sr content of 5.14 mg/l. 87Sr/86Sr ratios of Quaternary groundwater samples are in the range of Oligocene potash deposits and salt-bearing mining dumps. Hints for ascending deep fluids in shallow aquifers are not found.