Hollow CuCo2O4 anchored on porous reduced graphene oxide (PrGO) was prepared. The hollow CuCo2O4 polyhedron possessed plentiful catalytic active sites. CuCo2O4/PrGO composite showed high electrocatalytic activity for glucose oxidation. The CuCo2O4/PrGO based glucose sensor exhibited high sensitivity and application feasibility.