miRNA profiling of high, low and non-producing CHO cells during biphasic fed-batch cultivation reveals process relevant targets for host cell engineering
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- 作者：Fabian Stiefela ; b ; stiefel@hochschule-bc.de" class="auth_mail" title="E-mail the corresponding author ; Simon Fischera ; b ; simon.fischer@boehringer-ingelheim.com" class="auth_mail" title="E-mail the corresponding author ; Alexander Sczyrbac ; asczyrba@techfak.uni-bielefeld.de" class="auth_mail" title="E-mail the corresponding author ; Kerstin Ottea ; otte@hochschule-bc.de" class="auth_mail" title="E-mail the corresponding author ; Friedemann Hessea ; hesse@hochschule-bc.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：miRNA ; micro RNA ; cDNA ; complementary DNA ; CHO ; Chinese hamster ovary ; cgr ; Cricetulus griseus ; CPM ; counts per million ; DE ; differentially expressed ; FDR ; false discovery rate ; hsa ; Homo sapiens ; mmu ; Mus musculus ; mAB ; monoclonal antibody ; NGS ; next generation sequencing ; qRT-PCR ; quantitative real time polymerase chain reaction ; RIN ; RNA integrity number ; SEAP ; secreted alkaline phosphatase ; STR ; stirred tank bioreactor
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 May 2016
- 年：2016
- 卷：225
- 期：Complete
- 页码：31-43
- 全文大小：3273 K
文摘
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Establishment of miRNA profiles with next generation sequencing during a fed-batch cultivation.
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High time resolution of an approach with control and biphasic process conditions.
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Clear identification of miRNA involved during mild hypothermia.
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Phenotype analysis of 19 process relevant miRNAs.