Preparation of quaternary amine monolithic column for strong anion-exchange chromatography and its application to the separation of Enterovirus 71
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- 作者：Huimin Gua ; Dezhong Yina ; dezh_yin@nwpu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Jie Rena ; Baoliang Zhangb ; Qiuyu Zhangb ; qyzhang1803@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Monolithic column ; Strong anion-exchange chromatography ; Enterovirus 71 ; Quaternary amine
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 October 2016
- 年：2016
- 卷：1033-1034
- 期：Complete
- 页码：399-405
- 全文大小：1937 K
文摘
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Monolithic column with large convective pores and high dynamic binding capacity was prepared.
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Monolith interacts electrostatically with the proteins through quaternary amine groups.
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Strong anion-exchange chromatography was practiced to separate protein mixtures.
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Separation of EV71 from virus-proteins mixture was achieved.