Non-invasive assessment of ATP regeneration potential of the preserved donor liver: A ³¹P MRS study in pig liver

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• 作者：Kumar Changani ; K. ; Fuller ; Barry J. ; Bryant ; David J. ; Bell ; Jimmy D. ; Ala-Korpela ; Mika ; et. al.
• 关键词：Adenosine triphosphate regeneration ; Hypothermic reperfusion ; Liver ; Storage
• 刊名：Journal of Hepatology
• 出版年：1997
• 出版时间：February, 1997
• 年：1997
• 卷：26
• 期：2
• 页码：336-342
• 全文大小：716 K

文摘

We have developed a quick, non-invasive method for measuring the ability of an isolated preserved liver to regenerate high energy phosphate nucleotides without the need for biopsy. Using ³¹P MRS we have monitored the hepatic energetics of intact cold preserved pig liver using standard clinical harvesting and storage techniques. Following cold storage for 2 h the livers were hypothermically reperfused with oxygenated modified University of Wisconsin preservation fluid. Prior to reperfusion MRS detectable adenosine diphosphate plus adenosine triphosphate was negligible; however, the spectrum showed intense resonances from phosphomonoesters and inorganic phosphate, as a consequence of adenosine triphosphate hydrolysis during cold preservation. Following a 10-min period of hypothermic reperfusion, regeneration of adenosine triphosphate occurred with a concurrent decline in inorganic phosphate and phosphomonoester, both of which are associated with adenosine triphosphate synthesis. The capacity of the liver to regenerate adenosine triphosphate following a 24-h period of cold storage was reduced by approximately 40 % (p<0.01) of the total amount achieved following the shorter cold storage time. Adenosine triphosphate regeneration rates were biphasic and were decreased upon prolonged storage, with the initial rate being reduced from 40.6 · 10⁻² · min⁻¹ (standard deviation (sd) 2.70 · 10⁻² · min⁻¹) to 14.8 · 10⁻² · min⁻¹ (sd; 2.4 · 10⁻² · min⁻¹) and the secondary rate from 1.77 · 10⁻² · min⁻¹ (sd; 0.18 · 10⁻² · min⁻¹) to 0.84 · 10⁻² · min⁻¹ (sd; 0.45 · 10⁻² · min⁻¹). MR images of the liver during the period of hypothermic reperfusion were also performed providing an assessment for the degree of hepatic vascular perfusion. This non-invasive, ³¹P MRS assessment of hepatic energetics in a clinically relevant animal model has great potential for the understanding of graft preservation injury.