New strategy for large-scale preparation of the extracellular domain of tumor-associated antigen HAb18G/CD147 (HAb18GED)

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• 作者：Zheng  ; Zhang¹ ;   ; ^&dagger ; ; Xiangmin  ; Yang¹ ;   ; ^&dagger ; ; Hushan  ; Yang² ; Xiaoling  ; Yu¹ ; Yu  ; Li¹ ; Jinliang  ; Xing¹ ;   ; ^&Dagger ; ;   ; ^{Xingjinliang@163.com} ; Zhinan  ; Chen¹ ;   ; ^&Dagger ;
• 关键词：HAb18G/CD147 ; protein expression ; site-directed integration system
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2011
• 出版时间：January 2011
• 年：2011
• 卷：111
• 期：1
• 页码：1-6
• 全文大小：688 K

文摘

HAb18G/CD147, a cancer-associated biomarker, can promote tumor growth, invasion and metastasis. Here, we established a new strategy to express the extracellular domain of HAb18G/CD147 (HAb18GED) by using the FRT/Flp-In recombinase-based site-directed integration system. Two clones expressing HAb18GED were established, and 600 mg HAb18GED was obtained with more than 95 % purity. Our results showed that purified HAb18GED exhibited strong activities to stimulate the secretion of pro-MMP2 and MMP-2 and promote the invasion of HCC cells. Overall, our study effectively overcame the difficulty of large-scale HAb18GED preparation and laid a solid foundation for the further studies on structure and functions of HAb18G/CD147.